Cell-Free Synthesis of Selenoproteins in High Yield and Purity for Selective Protein Tagging

The selenol group of selenocysteine is much more nucleophilic than the thiol group of cysteine. Selenocysteine residues in proteins thus offer reactive points for rapid post-translational modification. Herein, we show that selenoproteins can be expressed in high yield and purity by cell-free protein synthesis by global substitution of cysteine by selenocysteine. Complete alkylation of solvent-exposed selenocysteine residues was achieved in 10 minutes with 4-chloromethylene dipicolinic acid (4Cl-MDPA) under conditions that left cysteine residues unchanged even after overnight incubation. Gd^III−Gd^III distances measured by double electron–electron resonance (DEER) experiments of maltose binding protein (MBP) containing two selenocysteine residues tagged with 4Cl-MDPA-Gd^III were indistinguishable from Gd^III−Gd^III distances measured of MBP containing cysteine reacted with 4Br-MDPA tags.     

世界各国(或地区)推进RFID的策略

介绍了一些国家和地区发展RFID的政策和推进措施，总结了各国发展RFID的共性。     

NFC移动通信终端的研究与应用

随着计算机网络及通信技术的发展,人们对短距离无线通信(NFC,Near Field Communication)的需求迅速增加。文中介绍了NFC技术的基本特点,给出了NFC的技术架构,然后在其基础上研究了NFC移动通信终端以及功能模块,并且给出了具体的应用,最后对NFC移动通信终端的应用前景做出展望。     

Depth and temperature selection of lake charr (Salvelinus namaycush) ecotypes in Lake Superior revealed by popup satellite archival tags

Lake charr exhibit morphological diversity in large North American lakes, largely attributed to habitat partitioning. Bathythermal habitats of lean lake charr have been assessed but remain largely unknown for other lake charr ecotypes. Popup satellite archival tags (PSATs) were used to determine depth and temperature profiles of lean (n = 15), siscowet (n = 16) and redfin (n = 3) lake charr ecotypes in Lake Superior during segments of the year. Monthly median depths of leans were < 20 m throughout the year while the median depth of redfins was 24–32 m (June-September). Monthly median depth of siscowets ranged from 103 to 204 m but they exhibited four distinctive patterns: deep (>80 m), shallow (<10 m), extreme vertical movements (>80–0 m), and high frequency vertical movements between 100 and 125 m. Siscowets were the most stenothermal with habitat temperatures generally 4–5 °C but not exceeding 12 °C. Leans were found as low as 0 °C (January-March) and up to 15 °C (July-September). Median monthly habitat temperatures for redfins were 1–3 °C colder than leans during July-September. PSATs confirmed that siscowets exhibit extreme vertical movements and may feed near the surface. The timing and duration of the extreme vertical movements exhibited by siscowets did not appear to be associated with time of day (i.e., not diel vertical movements) and may be described as opportunistic movements presumably related to foraging. Data obtained from the PSATs reinforces the view that lake charr are highly adaptive and can thrive in cold, oligotrophic lakes by optimizing the use of the entire water column.     

基于物联网技术的智慧种业全周期追溯防伪管理系统

利用RFID技术、电子技术、计算机技术、数据库技术、3G/4G通讯技术、特种标签制造技术及3D仿真技术等,构建了智慧种业全周期追溯防伪管理系统,该系统在种子生产包装环节将电子玉米标签随种子混装于包装袋内,同时将品种、批次、生产日期等信息进行赋码,充分利用超高频远距离批量识别特性,实现批量出入库、物流各环节的全程监控,利用非可视、不接触识读特性实现终端防伪查询及隐蔽式窜货稽查,很好地解决了目前种子行业防伪、防窜货手段所存在的易仿造、易破坏的问题,为国家种业安全提供一个新型的防伪、防窜货技术平台。     

基于Tiff图像数字化档案的研究

Tiff文件格式是一个丰富的图像格式,可以支持多种图像,现在已经成为存储档案数字化图像的标准格式.现有的应用程序都可以很好的支持Tiff图像格式.本文从Tiff的结构、特性、分类等几个方面,对Tiff文件进行了详细的分析.在支持传真的Class-F分类基础上,通过配置Tiff标签和在图像数据区域附加图像描述信息,对基于Tiff图像的数字化档案进行研究,并把此研究应用于焊接培训中,并取得了预期的效果.     

基于NFC的考勤系统设计与实现

本文利用NFC设计了一套利用NFC手机进行考勤的考勤系统,它由考勤机、安卓App和上位机组成。     

应用射频技术的商场智能导购系统

设计的电子导购系统,包括电子标签的选择,嵌入式 Android 系统的选择,电子导购系统功能模块的组成。同时设计了客户端的两大功能模块,包括促销信息功能模块的设计,电子地图定位模块的设计。讨论了定位RFID 标签的布局,并利用 RSSI 来评估其接收的信号强度并计算出标签和读写器的距离,根据相邻的四个标签的坐标,采用极大似然估计方法获得智能购物车的位置,能够用于商场内精准的定位。     

Design of a Rotary Ring APP Information Capturer for Sightseeing PlacemSpecialized Tourism Services for Sun-Moon Lake

The APP （Application） store at the Android market is very popular and attractive for most of current smart phone users. A rotary ring type of APP named as Loving SML （Loving Sun-Moon Lake--＂爱上日月谭＂） is an instant information capturer to capture the instant traffic tourism information （info） services and serve as an interactive device for user to know the instant traffic information of SML area where you may want to go. The tourism service is specialized on i3 travel, which is identified as innovation, intelligent, and interesting for all of the visitors coming from all over the world. The captured instant information is provided by the sightseeing cloud from SML-NSA （National Scenic Area） with the integration of infrastructure of VD （vehicle detector）, CMS or VMS （changeable （or variable） message sign）, CCTV （closed circuit television）, AVI （automatic vehicle identification）. The technique of AR （augmented reality） is also used to identify how many sparkling places in front of you that you may visit. One can easily know the road performance ahead from you before you plan a trip.     

融合NFC的3G智能卡系统

针对NFC技术和3G智能卡技术的可融合性,提出一种多功能3G智能卡系统。介绍近场通信的连接和传输及3G智能卡,研究两者融合的方法。该系统以3G智能卡的USIM卡为载体,利用近场通信技术将非接触的应用功能应用在USIM卡上,给出融合系统模型和设计方案。分析结果表明,该系统具有较好的融合性。