响应面优化明胶-壳聚糖可食性复合膜的制备

目的制备明胶-壳聚糖可食性复合膜,以期为不同的实际应用提供实验数据和理论依据。方法利用明胶和壳聚糖的分子结构特点以及聚乙二醇400(PEG-400)等添加物质的性能构效关系,采用溶液浇注法制得明胶-壳聚糖复合膜,研究明胶-壳聚糖的质量分数、明胶与壳聚糖的质量比、PEG-400质量分数对复合膜性能的影响,并根据Box-Benhnke中心组合设计原理设计响应面分析试验,探讨各因素之间的交互作用,探索出明胶-壳聚糖膜的最佳工艺配方。结果膜的物理性能受明胶-壳聚糖质量分数、明胶与壳聚糖的质量比、PEG-400质量分数以及两两交互之间的影响较大。当明胶-壳聚糖的质量分数为8.5%,明胶与壳聚糖的质量比为7∶3,PEG-400质量分数为10%时,膜的拉伸强度为19.53 MPa,断裂伸长率为39.82%。结论所制作膜的透明度、光泽度、表面光滑度、气味及物理性能等各项表征均为良好。     

Effect of transglutaminase and EDC on biodegradation of fish gelatin and gelatin-chitosan films

The subject of the study was analysis of enzymatic degradation of fish gelatin and fish gelatin-chitosan films cross-linked with transglutaminase (TGase) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC). Unmodified gelatin films were almost completely hydrolysed by trypsin and proteinase N, and in about 60% by pepsin. Gelatin films modified with TGase in concentration of 0.2 mg/ml and with EDC (30 mM) were hydrolysed by trypsin in 90–100% while by pepsin in about 40 and 25%, respectively. Fish gelatin from gelatin-chitosan films, unmodified and modified with TGase, was almost completely hydrolysed by trypsin. The presence of chitosan in two-components films cross-linked with EDC decreased hydrolysis by pepsin and trypsin, respectively, by about 65, and 20% more in comparison to that of one-component chemical modified films. Applied modifications had no significant influence on degradation of fish gelatin and gelatin-chitosan films by proteinase N.     

明胶-壳聚糖共混载药膜的体外释药特性

溶剂挥发法制备明胶-壳聚糖（GICS）共混膜,作为药物持续释放载体治疗眼穿通伤.氢化可的松琥珀酸钠（HSS）作为模型药物.制备明胶和壳聚糖组成比例（体积比）为1：3和1：4,载药量一样的GICS药膜.pH＝7.4的缓冲溶液中降解实验显示,随着明胶加入量增加,提高了膜的溶解性,降解加快.体外释药实验显示药物释放与时间平方根呈函数关系,药物呈缓慢释放,能维持10 d以上的有效药物浓度,初步兔眼实验显示,该膜组织相容性好,治疗眼穿通伤效果明显.