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91.
Fare TL Coffey EM Dai H He YD Kessler DA Kilian KA Koch JE LeProust E Marton MJ Meyer MR Stoughton RB Tokiwa GY Wang Y 《Analytical chemistry》2003,75(17):4672-4675
A data anomaly was observed that affected the uniformity and reproducibility of fluorescent signal across DNA microarrays. Results from experimental sets designed to identify potential causes (from microarray production to array scanning) indicated that the anomaly was linked to a batch process; further work allowed us to localize the effect to the posthybridization array stringency washes. Ozone levels were monitored and highly correlated with the batch effect. Controlled exposures of microarrays to ozone confirmed this factor as the root cause, and we present data that show susceptibility of a class of cyanine dyes (e.g., Cy5, Alexa 647) to ozone levels as low as 5-10 ppb for periods as short as 10-30 s. Other cyanine dyes (e.g., Cy3, Alexa 555) were not significantly affected until higher ozone levels (> 100 ppb). To address this environmental effect, laboratory ozone levels should be kept below 2 ppb (e.g., with filters in HVAC) to achieve high quality microarray data. 相似文献
92.
Taylor Steven; Zvolensky Michael J.; Cox Brian J.; Deacon Brett; Heimberg Richard G.; Ledley Deborah Roth; Abramowitz Jonathan S.; Holaway Robert M.; Sandin Bonifacio; Stewart Sherry H.; Coles Meredith; Eng Winnie; Daly Erin S.; Arrindell Willem A.; Bouvard Martine; Cardenas Samuel Jurado 《Canadian Metallurgical Quarterly》2007,19(2):176
Accumulating evidence suggests that anxiety sensitivity (fear of arousal-related sensations) plays an important role in many clinical conditions, particularly anxiety disorders. Research has increasingly focused on how the basic dimensions of anxiety sensitivity are related to various forms of psychopathology. Such work has been hampered because the original measure--the Anxiety Sensitivity Index (ASI)--was not designed to be multidimensional. Subsequently developed multidimensional measures have unstable factor structures or measure only a subset of the most widely replicated factors. Therefore, the authors developed, via factor analysis of responses from U.S. and Canadian nonclinical participants (n = 2,361), an 18-item measure, the ASI-3, which assesses the 3 factors best replicated in previous research: Physical, Cognitive, and Social Concerns. Factorial validity of the ASI-3 was supported by confirmatory factor analyses of 6 replication samples, including nonclinical samples from the United States and Canada, France, Mexico, the Netherlands, and Spain (n = 4,494) and a clinical sample from the United States and Canada (n = 390). The ASI-3 displayed generally good performance on other indices of reliability and validity, along with evidence of improved psychometric properties over the original ASI. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
93.
Genetic algorithms are a technique for search and optimization based on the Darwinian principle of natural selection. They are iterative search procedures that maintain a population of candidate solutions. The best or most fit solutions in that population are then used as the basis for the next generation of solutions. The next generation is formed using the genetic operators reproduction, crossover, and mutation. Genetic algorithms have been successfully applied to engineering search and optimization problems. This paper presents a discussion of the basic theory of genetic algorithms and presents a genetic algorithm solution of a lumber cutting optimization problem. Dimensional lumber is assigned a grade that represents its physical properties. A grade is assigned to every board segment of a specific length. The board is then cut in various locations in order to maximize its value, A genetic algorithm was used to determine the cutting patterns that would maximize the board value. 相似文献
94.
Teleological explanations are based on the assumption that an object or behavior exists for a purpose. Two studies explored the tendency of adults and first-, second-, and fourth-grade elementary-school children to explain the properties of living and nonliving natural kinds in teleological terms. Consistent with the hypothesis that young children possess a promiscuous teleological tendency, Study 1 found that children were more likely than adults to broadly examine the properties of both living and nonliving natural kinds in teleological terms, although the kinds of functions that they endorsed varied with age. Study 2 was an attempt to reduce children's broad teleological bias by introducing a pretrial that described, in nonteleological terms, the physical process by which nonliving natural kinds form. In spite of this attempt, Study 2 replicated the effects of Study 1, with only fourth graders showing any shift in preference for teleological explanation. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
95.
Raven Mary A.; Necessary Brian D.; Danluck Deborah A.; Ettenberg Aaron 《Canadian Metallurgical Quarterly》2000,8(1):117
People report that ethanol improves the experience produced by cocaine. This effect may be attributable to cocaethylene (CE), a cocaine metabolite formed only in the presence of ethanol. To test this, rats were trained to run an alley for a single intravenous dose of either cocaine (0.5–2.0 mg/kg) or an equimolar dose of CE (0.75–2.88 mg/kg). The rats' start latency and running speed measured the reinforcing effects of the drugs and the number of times rats approached but failed to enter the goal box (i.e., approach-avoidance retreats) indexed anxiety. Rats reinforced with CE had shorter start latencies and faster running speeds and exhibited fewer "retreats" than cocaine-reinforced rats. These results suggest that CE is more reinforcing and less anxiogenic than cocaine and hence may account for the combined effects of cocaine and ethanol in humans. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
96.
John A. Kuprenas Rodney K. Haraga Deborah L. DeChambeau Jonis C. Smith 《Canadian Metallurgical Quarterly》2000,16(5):27-33
This work introduces a new training program performance assessment methodology for engineering organizations that is based upon needs assessment, types of training, and training effectiveness measures. The methodology checks whether predefined objectives of a company are identified through a needs assessment. Effectiveness is judged on whether the types of training and coursework content are formulated to fulfill the needs assessment objectives, whether the coursework is available to the proper employees, whether the courses are attended and understood by the employees, and whether the coursework content is utilized in the work place to meet the needs assessment objectives of the company. A case study of three training programs conducted within a public sector engineering organization is included to demonstrate the methodology and evaluate both the three programs and the methodology effectiveness. Conclusions suggest improvements to future training programs for the public sector engineering organization, as well as describe potential enhancements to the effectiveness measures of the methodology that can be recognized through further study of existing training programs. 相似文献
97.
This article explores the meanings of nature, and examines how understandings of this term inform the field of psychology. In this period of high, late, or post-modernity, many of the "givens" become contested, and perhaps nothing has become more contested than nature itself. There is a threefold purpose to this paper. This first goal is to unravel two types of nature in psychology, to be distinguished as nature and Nature. The second aim is to discuss how these types of nature/Nature have been distinguished epistemologically, ontologically and methodologically within the field. The final purpose is to suggest points of unity within nature and Nature in psychology. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
98.
There is a long and varied usage of self-help housing policy in South Africa, dating from colonial times when Africans were dumped in locations and left to build their own housing, to the present post apartheid reconstruction. The paper documents and periodises the adoption of self-help strategies for housing the African population of South African cities throughout the 20th century. Exploration of the practice of self-help housing practices in Johannesburg suggests that owner construction is a prevailing method of social engineering, whose acceptance or rejection reflects perceived political and economic advantages for the state and the private sector, and is not a simple response to a shortage of affordable shelter among the poor. 相似文献
99.
Sullivan Edith V.; Fama Rosemary; Shear Paula K.; Cahn-Weiner Deborah A.; Stein Maria; Zipursky Robert B.; Pfefferbaum Adolf 《Canadian Metallurgical Quarterly》2001,15(3):342
Motor abnormalities occur in schizophrenia (SZ) and may arise from striatal dysfunction. This study examined whether the pattern of performance on simple and complex motor abilities in SZ was similar to that of patients with Parkinson's disease (PD). Quantitative tests of speeded movement and motor and cognitive sequencing were used to assess 25 SZ, 16 PD, and 84 normal controls (NCs). Sequencing performance was also examined with motor rigidity taken into account. Compared with the NC group, the SZ and PD groups were impaired on measures of motor rigidity and motor sequencing. With rigidity accounted for, the SZ group was significantly more impaired than the PD group on motor sequencing; cognitive and motor processes contributed to the motor deficit. Cognitive sequencing performance predicted motor sequencing performance in PD but not SZ. Although both SZ and PD resulted in significant motor and cognitive sequencing deficits, the pattern and correlates of these deficits differ, suggesting that the affected neural systems underlying motor deficits in SZ are different from those involved in PD. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
100.
Deborah L. Franke Eugene C. Cole Keith E. Leese Karin K. Foarde Michael A. Berry 《Indoor air》1997,7(1):41-54
Abstract A study was conducted to characterize the indoor environment of a multifloor, multiuse, nonproblem, noncompliant building through long-term monitoring for biological, chemical, and particulate pollutants. The study also assessed the effects of cleaning on indoor air quality by providing a program to monitor baseline levels, providing a rigorous (deep) cleaning of the building, and then continuing to monitor after implementation of a standardized, improved, cleaning program. To assess the effectiveness of the cleaning program, air, surface, and dust data from monitoring prior to the cleaning program were compared with those obtained while the improved housekeeping program was in place. Correlations between pollutants and other environmental factors were studied. The data suggest that the improved cleaning program contributed to indoor air quality through the reduction of airborne dust mass, total volatile organic compounds, and culturable bacteria and fungi. 相似文献