Review of Topographic and Soil Conditions for Liquefaction-Related Damage Induced by the 2007 Off Mid-Niigata Earthquake

The 2007 Niigata-ken Chuetsu-oki (Off Mid-Niigata) earthquake caused the liquefaction of the sandy soil distributed near the coast of the Japan Sea in the middle of Niigata Prefecture. The liquefaction-induced damage occurring in many residential areas was investigated in detail by means of victim interviews, visual inspections, Swedish Weight Sounding tests, Standard Penetration Tests, old topographical map examinations, etc. Based on in situ soil investigations, the liquefied soil layers were estimated for each area. As a result, it was confirmed that the liquefied areas were mainly sand dune hinterlands, flood plains, reclaimed old river channels and sandy fills with high groundwater table. Among them, damage was especially serious on land having an inclined ground surface due to the flow of foundations, on the cut-fill borders of artificially developed land due to landslides and at the toes of sand dune slopes due to the thrust of the sliding soil and/or the collision of the sliding soil with objects. These investigation results also revealed that soil improvement by cement mixed columns is an effective countermeasure against liquefaction-induced damage unless lateral spreading of the subsoil arises.     

Formation of Apatite Coatings on an Artificial Ligament Using a Plasma- and Precursor-Assisted Biomimetic Process

A plasma- and precursor-assisted biomimetic process utilizing plasma and alternate dipping treatments was applied to a Leed-Keio artificial ligament to produce a thin coating of apatite in a supersaturated calcium phosphate solution. Following plasma surface modification, the specimen was alternately dipped in calcium and phosphate ion solutions three times (alternate dipping treatment) to create a precoating containing amorphous calcium phosphate (ACP) which is an apatite precursor. To grow an apatite layer on the ACP precoating, the ACP-precoated specimen was immersed for 24 h in a simulated body fluid with ion concentrations approximately equal to those in human blood plasma. The plasma surface modification was necessary to create an adequate apatite coating and to improve the coating adhesion depending on the plasma power density. The apatite coating prepared using the optimized conditions formed a thin-film that covered the entire surface of the artificial ligament. The resulting apatite-coated artificial ligament should exhibit improved osseointegration within the bone tunnel and possesses great potential for use in ligament reconstructions.     

Preparation of griseofulvin nanoparticle suspension by high-pressure homogenization and preservation of the suspension with saccharides and sugar alcohols

Aim: We have attempted to micronize drug particles with a particle size of less than 100 nm and maintain the particle size of their suspension to improve the solubility and bioavailability of poorly water-soluble drugs. Furthermore, the method of freeze-drying nanoparticles was applied to maintain particulate nature of nanoparticles containing various saccharides and sugar alcohols for a long time. Method: Griseofulvin (GF)–lipid nanoparticle suspension is prepared using GF and a lipid by high-pressure homogenization. The particle size of the obtained GF–lipid nanoparticle suspension is maintained constant by freeze-drying. Result: The mean particle size of GF–lipid nanoparticles prepared by high-pressure homogenization is approximately 60 nm. The mean particle size remains less than 100 nm for 1 month. The GF–lipid nanoparticle suspension containing xylitol, trehalose, or sucrose is freeze-dried to maintain the particulate nature. The mean particle size of the rehydrated suspension is lower than that of the rehydrated suspension containing erythritol or lactose. In particular, it is new knowledge to have found that an aggregation is minimized by adding xylitol which is sugar alcohol. The minimum concentration of xylitol, trehalose, and sucrose required to maintain a constant particle size by rehydration is 3%, 3%, and 5% (w/v), respectively.     

Fabrication of DNA-antibody–apatite composite layers for cell-targeted gene transfer

Surface-mediated gene transfer systems using apatite (Ap)-based composite layers have received increased attention in tissue engineering applications owing to their safety, biocompatibility and relatively high efficiency. In this study, DNA-antibody–apatite composite layers (DA–Ap layers), in which DNA and antibody molecules are immobilized within a matrix of apatite nanocrystals, were fabricated using a biomimetic coating process. They were then assayed for their gene transfer capability for application in a specific cell-targeted gene transfer. A DA–Ap layer that was fabricated with an anti-CD49f antibody showed a higher gene transfer capability to the CD49f-positive CHO-K1 cells than a DNA–apatite composite layer (D–Ap layer). The antibody facilitated the gene transfer capability of the DA–Ap layer only to the specific cells that were expressing corresponding antigens. When the DA–Ap layer was fabricated with an anti-N-cadherin antibody, a higher gene transfer capability compared with the D–Ap layer was found in the N-cadherin-positive P19CL6 cells, but not in the N-cadherin-negative UV♀2 cells or in the P19CL6 cells that were pre-blocked with anti-N-cadherin. Therefore, the antigen–antibody binding that takes place at the cell–layer interface should be responsible for the higher gene transfer capability of the DA–Ap than D–Ap layer. These results suggest that the DA–Ap layer works as a mediator in a specific cell-targeted gene transfer system.     

Development of an early estimation method for predicting later osteogenic differentiation activity of rat mesenchymal stromal cells from their attachment areas

Cell morphology has received considerable attention in recent years owing to its possible relationship with cell functions, including proliferation, differentiation, and migration. Recent evidence suggests that extracellular environments can also mediate cell functions, particularly cell adhesion. The aims of this study were to investigate the correlation between osteogenic differentiation activity and the morphology of rat mesenchymal stromal cells (MSCs), and to develop a method of estimating osteogenic differentiation capability of MSCs on biomaterials. We measured the attachment areas of MSCs on substrates with various types of surface after 2 h of seeding, and quantified the amount of osteocalcin secreted from MSCs after 3 weeks of culture under osteogenic differentiation conditions. MSCs with small attachment areas showed a high osteogenic differentiation activity. These findings indicate that cell attachment areas correlate well with the osteogenic differentiation activity of MSCs. They also suggest that the measurement of cell attachment areas is useful for estimating the osteogenic differentiation activity of MSCs and is a practical tool for applications of MSCs in regenerative medicine.     

Synthesis of high concentration colloid solution of silica-coated AgI nanoparticles

Methods for high concentration silica-coated silver iodide (AgI/SiO2) particles, which could be practically used as X-ray contrast agent, were examined. The first was a single-step method, which was to prepare AgI nanoparticles at an AgI concentration of 5 x 10(-3) M and coat the AgI nanoparticles with silica shell by a St?ber method. The second was a multiple-step method, which was to repeat a step for preparing a AgI/SiO2 particle colloid solution with 10(-3) M AgI 5 times for adjusting a final AgI concentration to 5 x 10(-3) M. In the two methods, dominant particle aggregation took place, though core-shell particles were also produced. The third was a salting-out method, which was to salt out AgI/SiO2 particles in their colloid solution prepared at an AgI concentration of 10(-3) M, remove supernatant by decantation, and redisperse the particles in a fresh solvent. Consequently, AgI/SiO2 particles with an AgI concentration as high as 0.05 M were successfully prepared with the salting-out method, and their core-shell structure was not damaged during the salting-out.     

Exact optimal solutions of the minisum facility and transfer points location problems on a network

We consider hierarchical facility location problems on a network called Multiple Location of Transfer Points (MLTP) and Facility and Transfer Points Location Problem (FTPLP), where q facilities and p transfer points are located and each customer goes to one of the facilities directly or via one of the transfer points. In FTPLP, we need to find an optimal location of both the facilities and the transfer points while the location of facilities is given in MLTP. Although good heuristics have been proposed for the minisum MLTP and FTPLP, no exact optimal solution has been tested due to the size of the problems. We show that the minisum MLTP can be formulated as a p‐median problem, which leads to obtaining an optimal solution. We also present a new formulation of FTPLP and an enumeration‐based approach to solve the problems with a single facility.     

Cutting of polished single-crystal silicon by wire electrical discharge machining

Smoothly polished single-crystal silicon plates were cut by wire electrical discharge machining (WEDM) in water and in oil in order to investigate the effect of WEDM on the polished surfaces. For cutting in water, polished surfaces near cut sections have chips and cracks, and are extremely rough; the rough regions are upheaved. Examinations suggest that the upheaved region is silicon dioxide and results from oxidization of the surfaces by WEDM. Moreover, the polished surfaces far from the cut section are somewhat rough. For cutting in oil, polished surfaces near a cut section are smooth and almost flat although they have chips and cracks. These findings indicate the WEDM in oil is better than that in water for cutting polished single-crystal silicon to obtain high-quality surfaces.     

Rejection properties of aromatic pesticides with a hollow-fiber NF membrane

NF membranes have been drawing much attention in the field of drinking water treatment because they can remove hazardous organic micro-pollutants such as pesticides and THM precursors, as well as hardness. The rejection properties for 10 kinds of aromatic pesticides (0.045–0.194 mg·L⁻¹) were investigated with a bench-scale flow cell equipped with a hollow-fiber NF membrane (HNF-1, the nominal desalting degree 35% at 0.3 MPa). Both the permeate and retentate were returned to the feed tank, and separation experiments were conducted for 10 days. Although the feed and retentate concentrations decreased in the first half of the experimental period, stable rejections were obtained in the latter half of the period. The rejections were calculated based on the average concentrations of the retentate and feed, and the removals were on the initial feed concentration. The removals were in the range of 45.0–93.8%, but the rejections based on the feed concentrations were relatively low (41.0–88.4%). The logarithm of the solute permeability (log B) correlated linearly with the molecular weight of the pesticides. In addition, the batch-type adsorption experiments indicated the following results: all pesticides were adsorbed on the membrane and the adsorption properties were controlled not only by hydrophobicity (log P) of the pesticides but also by molecular planarity.