Studies of the neutralizing activity and avidity of anti-human immunodeficiency virus type 1 Env antibody elicited by DNA priming and protein boosting

DNA vaccination is an effective means of eliciting strong antibody responses to a number of viral antigens. However, DNA immunization alone has not generated persistent, high-titer antibody and neutralizing antibody responses to human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env). We have previously reported that DNA-primed anti-Env antibody responses can be augmented by boosting with Env-expressing recombinant vaccinia viruses. We report here that recombinant Env protein provides a more effective boost of DNA-initiated antibody responses. In rabbits primed with Env-expressing plasmids, protein boosting increased titer, persistence, neutralizing activity, and avidity of anti-Env responses. While titers increased rapidly after boosting, avidity and neutralizing activity matured more slowly over a 6-month period following protein boosting. DNA priming and protein immunization with HIV-1 HXB-2 Env elicited neutralizing antibody for T cell line-adapted, but not primary isolate, viruses. The most effective neutralizing antibody responses were observed after priming with plasmids which expressed noninfectious virus-like particles. In contrast to immunizations with HIV-1 Env, DNA immunizations with the influenza virus hemagglutinin glycoprotein did not require a protein boost to achieve high-titer antibody with good avidity and persistence.     

筒形件反旋工艺塑性流动刚塑性有限元分析

建立了筒形件反旋工艺平面变形刚塑性有限元分析的力学模型，比较了把反旋工艺视为正挤和本文力学模型条件下的速度场，有限元分析结果与反旋实际条件相符。     

“磁约束”的防垢、除垢机理分析

讨论了轴对称非均匀磁场对带电粒子的作用，分析了“磁约束”对防垢、除垢的微观机理，指出满足磁约束理论的轴对称非均匀磁场的防垢，除垢效果好的原因，正是由于在这种磁场中，带电粒子的运动被约束在一定磁面上的某一磁感应线附近做以该磁感应线为中心的螺旋运动，且正、负离子的回旋方向相反，从而形成内部相互作用较弱的大分子簇团不易吸附在管壁上，达到防垢目的；磁化水的渗透压较大，可起到除垢效果。     

烧结直接淬火对WC－TiC－Co合金WC相微观结构及力学性能的影响

本文以ＴＥＭ和ＸＲＤ为主要手段，研究ＷＣ－ＴｉＣ－Ｃｏ硬质合金（ＹＴ１４）在直接淬火后ＷＣ相的微观结构和微观应力，比较了常规烧结态与淬火态试样ＷＣ相之间的差异，探讨了直接淬火对合金力学性能的影响。结果表明，在保持合金其他性能不降低的同时，直接淬火的抗弯强度比常规烧结提高２８％左右。并首次发现，经直接淬火热处理的ＹＴＩ４合会中与Ｃｏ相相邻的ＷＣ晶粒边界出现滑移台阶，这是淬火过程中热应力使ＷＣ晶粒内位错在｛０００１｝密排面聚集，并可能沿｛０００１｝＜１１２０＞易滑移系统强滑移的结果。直接淬火试样的微观应力比重新加热淬火的低。     

无线局域网认证中密钥协商方案

针对无线局域网认证和密钥协商的安全性,文章介绍了椭圆曲线密码体制,对基于椭圆曲线密码体制的密钥协商协议进行研究,在EAP-TLS认证机制的基础上,提出一种无线局域网认证中基于ECC的密钥协商方案。     

一种具有等级结构的IBE密钥管理方案

基于标识的加密机制的出现解决了公钥密码体制中证书管理的问题,但由于用户公钥完全公开且不易更改,IBE机制的密钥管理问题成为讨论焦点.具有等级结构的身份密码体制(HIBE)的提出,解决了单个私钥生成中心(PKG)无法满足大用户量应用环境的负担和安全保障问题.文章提出的HKM方案解决了HIBE的PKG层次图中存在多父节点用户的复杂层次关系,并通过引入通配符的IBE方案,提出了基于WIBE的等级结构密钥管理方案.     

电子阅览室Squid上网过滤系统升级及运维工作精要

文中对本馆Squid上网过滤系统运行7年来的运维工作做了总结,这些艰辛得来的一线运维工作经验,对很多想使用该过滤系统,正使用该过滤系统的单位将会有很大的帮助。     

An incentive scheduling mechanism for peer-to-peer video streaming

P2P video streaming networks are found as a scalable solution and an alternative for traditional client–server based video streaming over the Internet. One of the significant issues affecting the success of any P2P streaming network is cooperation between peers. Practical observations have proved the prevalence of free riders in P2P networks that degrade their performance. To solve this problem, using incentive mechanisms, which encourage peers to contribute more in the network, is necessary. In this paper, we designed and proposed a distributed and scalable incentive mechanism for mesh based P2P video streaming networks. In the proposed approach the contribution of the peers is measured and maintained in a distributed fashion. Furthermore, we proposed an incentive sending side scheduler in which peers are served based on their contribution in the network. Our simulation evaluations show the efficiency of the proposed approach in improving the overall perceived video quality by the non-free rider nodes and consequently in the whole network.     

File size models for shared content over the BitTorrent Peer-to-Peer network

Peer-to-Peer (P2P) traffic has increased rapidly over the past few years, with file sharing providing the main drive behind such traffic. In this work we perform a measurement study of the content shared over the popular BitTorrent P2P file sharing network. We mathematically model the file size distributions of shared files after categorizing them into Audio, Video, Archive and CD image classes. For each of these categories we look into the most popular shared file formats and investigate their file size statistics. This provides an important milestone to building a realistic simulation framework for P2P systems, and for future analytical modeling of P2P networks.