Interfacial hybridization kinetics of oligonucleotides immobilized onto fused silica surfaces

Fused silica optical fibers have been used in an intrinsic mode optical configuration as biosensors for fluorescence based detection of hybridization of nucleic acids. In this work, the kinetics of hybridization of single-stranded oligonucleotides that were covalently immobilized were studied. The probe DNA was dT₂₀, and the target was Fluorescein-labeled non-complementary (dT₂₀) or complementary (dA₂₀) oligonucleotide. Chronofluorimetric monitoring of the adsorption and hybridization processes was used to investigate oligonucleotide films of different density, in different salt concentrations, at temperatures of 25 and 40 °C, with the concentration of the target DNA being 0.005–0.1 μM. Mathematical models based on first- and second-order Langmuir adsorption have been examined to describe both the adsorption and the hybridization processes. Experimental data were processed using the models, and the hybridization kinetics were calculated. Hybridization kinetics on these optical fiber DNA sensors was found to be up to three orders faster than results presented for a number of other experiments using different immobilization chemistries.     

Linux系统防火墙技术的实现

防火墙是提供网络安全性的重要手段之一,Linux提供了实现防火墙技术的工具包.分析了Linux系统中常用的包过滤防火墙技术--IPchains和IPtables.这两种防火墙技术作为两种基本的包过滤工具,实际上使用的是内核中的信息表,将来自非信任源的不必要的信息过滤出去.与IPchains相比,IPtables信息包过滤系统是最新的解决方案,也是第一个集成到Linux内核的解决方案.在许多方面,IPtables比IPchains灵活,它允许管理员控制更多的细节,例如记录日志等级.     

Bipolar electric fatigue behaviour as a function of field strength in antiferroelectric (Pb,Ba,La)(Zr,Sn,Ti)O3 ceramics

The bipolar electric fatigue behaviour of antiferroelectric ceramics with the composition of Pb_0.88Ba_0.10La_0.02(Zr_0.55Sn_0.35Ti_0.10)O₃ was investigated under various cycling fields. The material exhibits a degradation in the maximum field induced strain, a diffuse AFE-FE phase transition and an enhancement in the diffusion character of the FE-AFE phase transition due to electric cycling. Those variations increase with cycle number, indicating a logarithmic fatigue up to 10⁸ cycles. There is no indication for the variations to be recovered, and the symmetry of the negative and positive parts of the strain hysteresis loops still remains. Higher cycling field results in a stronger deterioration of the maximum field induced strain and a larger extent of diffusion in AFE-FE and FE-AFE phase transitions. The normalized maximum strain shows nearly the same scale of degradation when the materials are cycled at various electric fields. After a heat treatment at 500°C for 1 h, the variations in the AFE-FE and FE-AFE phase transition due to cycling disappear, whereas the maximum strain resumes almost to its original value. Electrochemical variations are considered to contribute to the main fatigue mechanism for the material under investigation.     

A study of semiconducting properties of hydrogen containing passive films

Mott–Schottky and photoelectrochemical measurements were used to explore the effects of hydrogen and chloride ions on the electronic properties of the passive film on X70 micro-alloyed steel in a solution of 0.5 M NaHCO₃. Mott–Schottky analyses have shown that hydrogen increases the capacitance and donor density, and decreases the flat band potential and the space charge layer thickness of the passive film. The photocurrent of the film is remarkably increased by hydrogen. The effects of hydrogen become more pronounced with an increase in the hydrogen charging current densities. Hydrogen has no noticeable effect on the band gap energy E_g and the process by which hole-electron pairs are photo-generated in the film. The presence of chloride ions in the solution produces some similar effects on the electronic properties of the passive film to those observed with hydrogen, but reduces the photocurrent and increases the band gap energy of the film. No significant synergistic effects on the electronic properties of the passive film were observed in the presence of hydrogen and Cl⁻. These results provide very useful information for elucidating the mechanism by which hydrogen changes the properties of passive film and then promotes localized corrosion.     

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章分析了川东地区与固井相关的地质地貌特征及井眼地质条件，介绍了常用的固井工艺技术，提出了川东地区固井所需要重点考虑的防气窜，防漏失，防硫化氢腐蚀及提高固井技术的几个重要难点问题，论述了解决这些问题的基本办法，通过固井实例，证明了这些办法的可行性和可靠性，因而对进一步提高该地区固井质量具有较广泛的实际指导意义。     

Modelling biological processes under anaerobic conditions through integrating titrimetric and off-gas measurements-applied to EBPR systems.

An innovative method for modelling biological processes under anaerobic conditions is presented and discussed. The method is based on titrimetric and off-gas measurements. Titrimetric data is recorded as the addition rate of hydroxyl ions or protons that is required to maintain pH in a bioreactor at a constant level. An off-gas analysis arrangement measures, among other things, the transfer rate of carbon dioxide. The integration of these signals results in a continuous signal which is solely related to the biological reactions. When coupled with a mathematical model of the biological reactions, the signal allows a detailed characterisation of these reactions, which would otherwise be difficult to achieve. Two applications of the method to the enhanced biological phosphorus removal processes are presented and discussed to demonstrate the principle and effectiveness of the method.     

猪场沼气工程改造技术研究

一、概况南平市种猪场位于延平区浆甲村 ,距市区 13km ,地处 316国道旁 ,面临沙溪口库湾边。年存栏猪760 0头 ,其中母猪 60 0头 ,后备种猪 2 5 0 0头 ,商品猪 4 5 0 0头 ,是闽北集种猪、商品猪生产及科研繁育于一体的副食品基地之一。该场日排猪粪便污水约140t,未建沼气工程前 ,直接排入水库 ,严重污染水体。1999年 ,种猪场采用厌氧发酵技术 ,处理猪粪便污水 ,兴建 4× 2 0 0m3沼气工程 ,技术落后 ,基本属于农村户用水压式沼气池的简单放大 ,猪粪便污水直接进入厌氧发酵池 ,出水直接排入水库。工程设计的主要问题是 :1 不设前处理装置 ,冲洗…     

压电量测系统漏电影响分析

本文通过对压电量测系统等效电路图的逐步简化,得出矩形脉冲信号的漏电方程;并对系统在各种情况下的时间常数,漏电相对误差进行了计算,最后就系统在校准和现场测试中经常碰到的几种情况,进行了定量估算,从而解决了压电量测系统在使用中的一些具体问题。     

高频数字锁相环的研究

论文阐述了１００ＭＨｚ数字锁相环的设计过程，用１０ＭＨｚ晶体振荡器对１００ＭＨｚ数字压控振荡器进行锁相，使１００ＭＨｚ输出信号指标得到很大改善。论文还分析了各单元电路，关键点时域波形测试，频谱测试。     

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川东北海相碳酸盐岩所生成的天然气主要聚集在喜山期形成的构造圈闭中。本文针对此特点,划分了背斜圈闭的成因类型,建立了海相碳酸盐岩孔隙、裂缝复合型背斜气藏模式,并指出了当前勘探的三大领域及寻找富集型气藏的前景。