The effect of optimized carbon source on the synthesis and composition of exopolysaccharides produced by Lactobacillus paracasei

This study aimed to predict the optimal carbon source for higher production of exopolysaccharides (EPS) by Lactobacillus paracasei TD 062, and to evaluate the effect of this carbon source on the production and monosaccharide composition of EPS. We evaluated the EPS production capacity of 20 strains of L. paracasei under the same conditions. We further investigated L. paracasei TD 062, which showed the highest EPS-producing activity (0.609 g/L), by examining the associated biosynthesis pathways for EPS. Genomics revealed that fructose, mannose, trehalose, glucose, galactose, and lactose were carbon sources that L. paracasei TD 062 could use to produce EPS. We identified an EPS synthesis gene cluster that could participate in transport, export, and sugar chain synthesis, and generate 6 sugar nucleotides. Experimental results showed that the sugar content of the EPS produced using fermentation with the optimized carbon source (fructose, mannose, trehalose, glucose, galactose, and lactose) increased by 115%. Furthermore, use of the optimized carbon source changed the monosaccharide content of the associated EPS. The results of enzyme activity measurements showed significant increases in the activity of 2 key enzymes involved in the glycoside synthesis pathway. Our study revealed that optimizing the carbon source provided for fermentation not only increased the production of EPS, but also affected the composition of the monosaccharides by increasing enzyme activity in the underlying synthesis pathways, suggesting an important role for carbon source in the production of EPS by L. paracasei TD 062.     

High-Tg porous polyimide films with low dielectric constant derived from spiro-(adamantane-2,9′(2′,7′-diamino)-fluorene)

Porous polyimide (PI) films with low dielectric constants and excellent thermal properties have been a pressing demand for the next generation of high-performance, miniature, and ultrathin microelectronic devices. A series of novel porous PI films containing fluorenyl-adamantane groups were prepared successfully via thermolysis of poly(ethylene glycol) (PEG) added in the PI matrix. The cross-sectional morphologies of porous PI films showed closed pores with diameters ranging from 135 to 158 nm, which were uniform and regular in shape without interconnectivity. These porous PI films exhibited excellent thermal properties with a glass-transition temperature at 376 °C whereas the 5% weight loss temperature in air excess of 405 °C due to enhanced rigidity afforded by fluorenyl-adamantane groups. Accompanied by thermolysis content of PEG increasing from 0 to 20 wt %, the density of porous PI films decreased, and the corresponding porosity grew significantly from 0 to 11.48%. Depending on porosity, the dielectric constant and dielectric loss of porous PI films significantly declined from 2.89 to 2.37 and from 0.050 to 0.021, respectively. These excellent properties benefit the as-prepared porous PI films for application as interlayer dielectrics, integrated circuit chips, or multichip modules in microelectronic fields. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136, 47313.     

试油井选层压裂新工艺及现场试验

设计新型可洗井封隔器，保障油井有反洗井通道，有效防止封隔器以上砂埋所引发的管柱砂卡事故，并对新型封隔器、控制开关、密封接头、同心大通径压力计托筒等井下工具组合优化。解决了多层油井任意选层压裂、求产、测井温一体化的难题。     

我国建筑石油沥青标准的沿革及现状

论述了我国建筑沥青标准的变化历程及现状,对我国建筑沥青的生产、产品质量情况及现行国家标准GB/T 494-1998《建筑石油沥青》的不足之处进行了分析。提出了今后应生产的建筑沥青品种及应参照美国材料与试验协会标准ASTM D312《屋顶用沥青的标准规格》、我国石化行业标准SH/T0002-1990(1998)《防水防潮石油沥青》,修订GB/T494- 1998的建议。以进一步达到增加建筑沥青牌号,提高建筑沥青质量和使用性能的目的,满足建筑工业的发展对沥青多牌号、高质量、使用性能好的要求。     

智能组合式充电机的开发与研制

针对集中供电式地震勘探仪器在地震勘探作业中对电瓶充电的特殊要求，我们研制出一种智能组合式充电机。文中阐述了智能式充电机的设计原理，介绍了其性能指标及使用效果。     

Magnetic Properties of Fe-Doped Rutile

Ball-milling method was applied to dissolve Fe into titanium dioxide (TiO₂). X-ray diffraction indicated the starting anatase changed to a rutile-type structure with oxygen deficiency after ball milling. Transmission electron microscopy and X-ray absorption experiments were conducted to examine the possible existence of magnetic impurities in the ball-milled powders after they were leached in HCl solutions. Temperature dependence of the resistivity shows semiconducting behavior and the magnetic hysteresis loops at 5 and 300 K exhibit ferromagnetic characteristics. Fe-doped TiO₂ films were also prepared by pulsed laser deposition. The magnetic properties of the films are discussed.     

UC3875在超声电源功率控制系统中的应用

根据超声波发生器的功率控制系统原理 ,阐述了移相控制策略对超声波发生器输出电压波形的影响 ,介绍了移相控制专用芯片UC3875的电路结构和使用设计方法。     

乙烯基硅氧烷改性苯丙乳液的研究

在苯乙烯 -丙烯酸酯乳液共聚反应后期 ,加入少量乙烯基硅氧烷 ,制得改性苯丙乳液 ,通过红外光谱初步确定了聚合物结构。研究了反应温度、反应时间、有机硅加入方式、乳化剂用量等因素对反应进程、乳液稳定性及涂膜性能的影响。     

高阶统计量地震子波估计建模

本文在反射系数序列为非高斯、平稳和统计独立的随机过程,地震子波为非因果、混合相位的假设条件下,分别应用滑动平均(MA)和自回归滑动平均(ARMA)模型对地震记录进行建模,并采用运算代价较小的基于高阶累积量的线性化求解方法——累积量矩阵方程法进行了子波提取和模型适应性的研究。数值模拟结果和实际地震数据处理结果表明:自回归滑动平均(ARMA)模型比滑动平均(MA)模型具有参数节省、模型更为高效的特点;累积量矩阵方程法可以有效地压制加性高斯噪声,但对累积量样本估计的准确性要求较高;如果累积量样本估计的误差和方差适度,结合自回归滑动平均(ARMA)模型描述的累积量矩阵方程法可以高效、准确地估计出地震子波。     

三峡工程三期截流三维施工仿真研究

三峡工程三期截流提前到 2 0 0 2年 11月上旬进行 ,截流难度显著增大。为了使截流工程顺利进行 ,以先进的计算机可视化技术为基础 ,采用IMAGIS软件生成三维地形图 ,用Creator建模形成导流明渠、纵向围堰及三期围堰等三维实体模型 ,最后通过运用虚拟现实技术 ,开发出三峡工程三期截流施工实时动态演示、查询系统。该系统能根据需要实现漫游 ,可以随意定位到感兴趣的区域作细部观察 ,且具有良好的交互性。通过施工仿真研究 ,将三期截流施工过程生动地显示在屏幕上 ,用以指导施工组织管理 ,为三期截流的决策和控制提供了有效的工具