A preparation method for observing intracellular structures by scanning electron microscopy

In order to observe intracellular structures by scanning electron microscopy, excess cytoplasmic matrix must be removed from the fractured surface of cells. Previously we reported an Osmium-DMSO-Osmium method devised for this purpose. This method is very effective in revealing intracellular structures, but requires osmium tetroxide for initial fixation with some consequent disadvantages. In the present study, a revised Osmium-DMSO-Osmium method is reported, in which an aldehyde mixture is used as the initial fixative instead of osmium tetroxide. As fixation is carried out by perfusion in this revised method, better preservation of fine structures is achieved than by the original method, especially in the central nervous tissue which tends to suffer from post-mortem degeneration. Moreover this method can be applied to cytochemical studies of intracellular structures with a scanning electron microscope (SEM). In this study, acid phosphatase of lysosomes is demonstrated in a coloured SEM micrograph.     

Hot Corrosion Behavior and Microstructural Change of Al-Gradient CoNiCrAlYSi Coatings, Produced by LVPS and Diffusional Processes

Conventional and gradient CoNiCrAlYSi coatings were produced by using low vacuum plasma spray and an additional step of diffusional over aluminizing (pack cementation) techniques on an Inconel-738 substrate. Hot corrosion of these coatings was investigated using Na₂SO₄–20wt%NaVO₃ molten salt at 880?°C for 800?h. Hot corrosion rate was determined by measuring the weight gain of the specimens at regular intervals for a duration of 20?h. X-ray diffraction, field emission scanning electron microscopy and electron probe micro analysis techniques were used to determine the nature of phases, investigation of the thermally grown oxide, examination of the surface attack and determination of the elemental distribution. The gradient coating showed better performance by re-healing alumina scale due to its possession of more β phase as Al reservoir. Results indicated that pack cementation process caused an increase in amount of aluminum-rich β phase and better hot corrosion properties of gradient coatings owing to the Al enrichment in the outer layer and rapid formation of protective oxide on the surface.     

Real-time 3D microtubule gliding simulation accelerated by GPU computing

A microtubule gliding assay is a biological experiment observing the dynamics of microtubules driven by motor proteins fixed on a glass surface. When appropriate microtubule interactions are set up on gliding assay experiments, microtubules often organize and create higher-level dynamics such as ring and bundle structures. In order to reproduce such higher-level dynamics on computers, we have been focusing on making a real-time 3D microtubule simulation. This real-time 3D microtubule simulation enables us to gain more knowledge on microtubule dynamics and their swarm movements by means of adjusting simulation parameters in a real-time fashion. One of the technical challenges when creating a real-time 3D simulation is balancing the 3D rendering and the computing performance. Graphics processor unit (GPU) programming plays an essential role in balancing the millions of tasks, and makes this real-time 3D simulation possible. By the use of general-purpose computing on graphics processing units (GPGPU) programming we are able to run the simulation in a massively parallel fashion, even when dealing with more complex interactions between microtubules such as overriding and snuggling. Due to performance being an important factor, a performance model has also been constructed from the analysis of the microtubule simulation and it is consistent with the performance measurements on different GPGPU architectures with regards to the number of cores and clock cycles.     

Evaluation of absorption of micro-droplets on paper for creation of paper-based microstructures

This study clarifies the absorption behavior of micro-droplets of water on treated paper to support the design of functional microstructures, such as electronics and micro-fluid channels, on paper. The period of time between when a micro-droplet of water ejected from an ink-jet head lands on the paper’s surface and its complete disappearance by absorption was defined as the micro-sizing degree (MSD), and an MSD measurement method was established. The MSD was evaluated using microscopic high-speed video images of the side view recorded every millisecond. Several grades of commercially available ink-jet paper media and laboratory sheets having different levels of water repellency prepared from a pulp and a sizing agent were examined. The MSD of commercial ink-jet papers, which are known to absorb water very quickly, was 3–6 ms. Weakly sized laboratory sheets exhibited a lower MSD of 2–3 ms. The absorption behavior was analyzed in terms of the capillary pressure with and without the Laplace pressure; the theoretical and experimental results agreed moderately well. The results indicated that the Laplace pressure cannot be neglected in the analysis. The MSD of a wet surface where a preceding micro-droplet had already landed was higher than that on a dry or partially wet surface, presumably because water remains inside pores for an unexpectedly long time.     

Towards the improvement of thermal efficiency in lignite‐fired power generation: Concerning the utilization of Polish lignite deposits in state‐of‐the‐art IGCC technology

Integrated coal Gasification Combined Cycle (IGCC) is the most advanced technology for coal‐fired power generation. The two‐stage entrained flow gasification process allows for the use of a wide range of coal, as long as the gasification temperature is above the ash melting point of a used fuel. In this gasification technology, lignite, which often has a low ash melting point, can be preferably utilized. However, ash fluidity is also another importance, because the behaviour of molten slag can diminish a stable ash discharge from a gasifier. As the eligibility of coal ash properties is a considerable factor, water physically and chemically kept in lignite (30 – 60% in mass) attributes to deteriorating gasification efficiency, because it causes significant heat loss and increasing oxygen consumption. Developing a thermal evaporative lignite drying method will be a necessary attempt to apply lignite to the coal gasification process. For those preceded objectives, coal and ash properties and drying characteristics of several grades of Polish lignite, extracted from Belchatow and Turow deposits, have been experimentally investigated in a preliminary study evaluating the applicability and consideration for its utilization in state‐of‐the‐art clean coal technology, IGCC. This paper particularly discusses the eligibility of Polish lignite from the perspective of the fusibility and fluidity of ash melts and the fundamental drying kinetics of lignite in superheated steam in the light of water removal. The viscosity of ash melts is measured at high temperature up to 1700 °C. In the drying tests, the significant influence of structural issues, because of the provenance and origin of lignite on the drying characteristics, was found by applying the method of sensitivity analysis of physical propensity. This paper concludes that the investigated Polish lignite has characteristics favourable for utilization in IGCC technology, once the precautions related to its high moisture have been carefully addressed. Copyright © 2016 John Wiley & Sons, Ltd.     

The isothermal conductivity improvement in zirconia-based ceramics under 24 GHz microwave heating

Abstract Under 24-GHz millimetre-wave irradiation heating ionic conductivity of zirconia base ceramics was up to 20 times higher than that of a conventionally-heated sample at the same temperature of 400 °C. The degree of enhancement could be altered by changing the stabilising atom from Y to Yb. Enhancement of ionic conduction was prominent in the setup condition of larger self-heating ratio and larger MMW absorbing materials. The isothermal improvement of ionic conductivity under MMW irradiation would be ascribed to the non-thermal effect.