Assessment of bottom-up sectoral and regional mitigation potentials

The greenhouse gas mitigation potential of different economic sectors in three world regions are estimated using a bottom-up approach. These estimates provide updates of the numbers reported in the Fourth Assessment Report of the Intergovernmental Panel on Climate Change (IPCC AR4). This study is part of a larger project aimed at comparing greenhouse gas mitigation potentials from bottom-up and top-down approaches. The sectors included in the analysis are energy supply, transport, industry and the residential and service sector. The mitigation potentials range from 11 to 15 GtCO₂eq. This is 26–38% of the baseline in 2030 and 47–68% relative to the year 2000. Potential savings are estimated for different cost levels. The total potential at negative costs is estimated at 5–8% relative to the baseline, with the largest share in the residential and service sector and the highest reduction percentage for the transport and industry sectors. These (negative) costs include investment, operation and maintenance and fuel costs and revenues at moderate discount rates of 3–10%. At costs below 100 US$/tCO₂, the largest potential reductions in absolute terms are estimated in the energy supply sector, while the transport sector has the lowest reduction potential.     

How many protein‐coding genes are there in the Saccharomyces cerevisiae genome?

We have compared the results of estimations of the total number of protein-coding genes in the Saccharomyces cerevisiae genome, which have been obtained by many laboratories since the yeast genome sequence was published in 1996. We propose that there are 5300-5400 genes in the genome. This makes the first estimation of the number of intronless ORFs longer than 100 codons, based on the features of the set of genes with phenotypes known in 1997 to be correct. This estimation assumed that the set of the first 2300 genes with known phenotypes was representative for the whole set of protein-coding genes in the genome. The same method used in this paper for the approximation of the total number of protein-coding sequences among more than 40 000 ORFs longer than 20 codons gives a result that is only slightly higher. This suggests that there are still some non-coding ORFs in the databases and a few dozen small ORFs, not yet annotated, which probably code for proteins.     

Optimisation and significance of ATP analysis for measuring active biomass in granular activated carbon filters used in water treatment

A method for determining the concentration of active microbial biomass in granular activated carbon (GAC) filters used in water treatment was developed to facilitate studies on the interactions between adsorption processes and biological activity in such filters. High-energy sonication at a power input of 40 W was applied to GAC samples for the detachment of biomass which was measured as adenosine triphosphate (ATP). Modelling of biomass removal indicated that a series of six to eight sonication treatments of 2 min each yielded more than 90% of the attached active biomass. The ATP concentrations in 30 different GAC filters at nine treatment plants in The Netherlands ranged from 25 to 5000 ng ATP cm(-3) GAC, with the highest concentrations at long filter run times and pretreatment with ozone. A similar concentration range was observed in nine rapid sand (RS) filters. ATP concentrations correlated significantly (p<0.05) with total direct bacterial cell counts in each of these filter types, but the median value of the ATP content per cell in GAC filters (2.1 x 10(-8) ng ATP/cell) was much lower than in the RS filters (3.6 x 10(-7) ng ATP/cell). Average biofilm concentrations ranging from 500 to 10(5) pg ATP cm(-2) were calculated assuming spherical shapes for the GAC particles but values were about 20 times lower when the surface of pores >1 microm diameter is included in these calculations. The quantitative biomass analysis with ATP enables direct comparisons with biofilm concentrations reported for spiral wound membranes used in water treatment, for distribution system pipes and other aquatic environments.     

A versatile wavelet domain noise filtration technique for medical imaging

In this paper, we propose a robust wavelet domain method for noise filtering in medical images. The proposed method adapts itself to various types of image noise as well as to the preference of the medical expert; a single parameter can be used to balance the preservation of (expert-dependent) relevant details against the degree of noise reduction. The algorithm exploits generally valid knowledge about the correlation of significant image features across the resolution scales to perform a preliminary coefficient classification. This preliminary coefficient classification is used to empirically estimate the statistical distributions of the coefficients that represent useful image features on the one hand and mainly noise on the other. The adaptation to the spatial context in the image is achieved by using a wavelet domain indicator of the local spatial activity. The proposed method is of low complexity, both in its implementation and execution time. The results demonstrate its usefulness for noise suppression in medical ultrasound and magnetic resonance imaging. In these applications, the proposed method clearly outperforms single-resolution spatially adaptive algorithms, in terms of quantitative performance measures as well as in terms of visual quality of the images.     

A logistic regression framework for information technology outsourcing lifecycle management

We present a methodology for managing outsourcing projects from the vendor's perspective, designed to maximize the value to both the vendor and its clients. The methodology is applicable across the outsourcing lifecycle, providing the capability to select and target new clients, manage the existing client portfolio and quantify the realized benefits to the client resulting from the outsourcing agreement. Specifically, we develop a statistical analysis framework to model client behavior at each stage of the outsourcing lifecycle, including: (1) a predictive model and tool for white space client targeting and selection—opportunity identification (2) a model and tool for client risk assessment and project portfolio management—client tracking, and (3) a systematic analysis of outsourcing results, impact analysis, to gain insights into potential benefits of IT outsourcing as a part of a successful management strategy. Our analysis is formulated in a logistic regression framework, modified to allow for non-linear input–output relationships, auxiliary variables, and small sample sizes. We provide examples to illustrate how the methodology has been successfully implemented for targeting, tracking, and assessing outsourcing clients within IBM global services division.Scope and purposeThe predominant literature on IT outsourcing often examines various aspects of vendor–client relationship, strategies for successful outsourcing from the client perspective, and key sources of risk to the client, generally ignoring the risk to the vendor. However, in the rapidly changing market, a significant share of risks and responsibilities falls on vendor, as outsourcing contracts are often renegotiated, providers replaced, or services brought back in house. With the transformation of outsourcing engagements, the risk on the vendor's side has increased substantially, driving the vendor's financial and business performance and eventually impacting the value delivery to the client. As a result, only well-ran vendor firms with robust processes and tools that allow identification and active management of risk at all stages of the outsourcing lifecycle are able to deliver value to the client. This paper presents a framework and methodology for managing a portfolio of outsourcing projects from the vendor's perspective, throughout the entire outsourcing lifecycle. We address three key stages of the outsourcing process: (1) opportunity identification and qualification (i.e. selection of the most likely new clients), (2) client portfolio risk management during engagement and delivery, and (3) quantification of benefits to the client throughout the life of the deal.     

The impact of drying and steaming processes on surface color changes of tension and normal beech wood

Abstract

Beech wood has a high frequency of defects such as red heartwood, reaction wood (tension wood). For the experimental measurements four logs without visible defects like red heartwood, which can noticeably affects the measurements' results were qualified. The drying medium temperature in the first phase of the process before the moisture content of the specimens fell below the FSP was maintained at 45?°C. Then, the temperature was gradually increased to the maximum value 65?°C. The results showed that different initial wood moisture content does not affect to final value of tension and normal wood. The colorimetric parameter L* was much higher for tension wood. After steaming, there were greater differences in the colorimetric parameters, respectively, a* and b*. After drying the difference of all colorimetric parameters between tension and normal wood was significantly less. The color changes were only noticed in the surface layers of specimens. Differences were small, and thus, the impact of the tension wood on the color changes was not confirmed. However, since the lightness of tension wood plays a key role for its visual detection, it may be adequate to only measure the lightness parameter (L*).     

Targeting Mitochondria in Diabetes

Type 2 diabetes (T2D), one of the most prevalent noncommunicable diseases, is often preceded by insulin resistance (IR), which underlies the inability of tissues to respond to insulin and leads to disturbed metabolic homeostasis. Mitochondria, as a central player in the cellular energy metabolism, are involved in the mechanisms of IR and T2D. Mitochondrial function is affected by insulin resistance in different tissues, among which skeletal muscle and liver have the highest impact on whole-body glucose homeostasis. This review focuses on human studies that assess mitochondrial function in liver, muscle and blood cells in the context of T2D. Furthermore, different interventions targeting mitochondria in IR and T2D are listed, with a selection of studies using respirometry as a measure of mitochondrial function, for better data comparison. Altogether, mitochondrial respiratory capacity appears to be a metabolic indicator since it decreases as the disease progresses but increases after lifestyle (exercise) and pharmacological interventions, together with the improvement in metabolic health. Finally, novel therapeutics developed to target mitochondria have potential for a more integrative therapeutic approach, treating both causative and secondary defects of diabetes.     

Mispredicting and misremembering: Patients with renal failure overestimate improvements in quality of life after a kidney transplant.

Objective: People tend to overestimate the impact that future events will have on their quality of life. In the case of a medical treatment like kidney transplant, this should result in biased predictions--overestimates of how much the transplant will benefit quality of life. The authors surveyed kidney transplant patients, both before and after transplant, to test whether they would overestimate the benefits of a successful transplant for their quality of life. Design: The authors interviewed 307 patients on a waiting list for cadaveric renal or renal-pancreatic transplant, and 195 patients one year after a successful transplant. A sub sample of patients were interviewed both before and after transplant. Main Outcome Measures: The survey included measures of quality of life, both in terms of an overall estimate (0-100), and across sub domains, including health, employment, and travel. Results: Cross-sectional results suggested that overall quality of life improved after transplant, but the predictions of pretransplant patients overestimated the magnitude of the improvement (p