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Dr. Anuchit Phanumartwiwath Dr. Chatchai Kesornpun Daranee Chokchaichamnankit Dr. Amnart Khongmanee Penchatr Diskul-Na-Ayudthaya Dr. Theetat Ruangjaroon Dr. Chantragan Srisomsap Prof. Prasat Kittakoop Prof. Jisnuson Svasti Prof. Somsak Ruchirawat 《Chembiochem : a European journal of chemical biology》2023,24(20):e202300268
Here we describe a novel catalyst-free 1,3-dipolar cycloaddition bioconjugation approach for chemical modification of proteins. The dehydroalanine (Dha)-containing protein reacts with nitrile oxides generated in situ through 1,3-dipolar cycloaddition in fully aqueous-buffered systems. This leads to the formation of a new isoxazoline ring at a pre-defined site (Dha) of the protein. Furthermore, the 1-pyrene isoxazoline-installed annexin V acts as a fluorescent probe, which successfully labels the outer cellular membranes of human cholangiocarcinoma (HuCCA-1) cells for detection of apoptosis. 相似文献