全文获取类型
收费全文 | 720篇 |
免费 | 25篇 |
国内免费 | 5篇 |
专业分类
电工技术 | 22篇 |
综合类 | 22篇 |
化学工业 | 108篇 |
金属工艺 | 14篇 |
机械仪表 | 11篇 |
建筑科学 | 12篇 |
矿业工程 | 91篇 |
能源动力 | 14篇 |
轻工业 | 97篇 |
水利工程 | 3篇 |
石油天然气 | 3篇 |
无线电 | 30篇 |
一般工业技术 | 63篇 |
冶金工业 | 226篇 |
原子能技术 | 6篇 |
自动化技术 | 28篇 |
出版年
2023年 | 2篇 |
2022年 | 6篇 |
2021年 | 11篇 |
2020年 | 11篇 |
2019年 | 6篇 |
2018年 | 9篇 |
2017年 | 12篇 |
2016年 | 15篇 |
2015年 | 8篇 |
2014年 | 13篇 |
2013年 | 22篇 |
2012年 | 20篇 |
2011年 | 55篇 |
2010年 | 39篇 |
2009年 | 31篇 |
2008年 | 18篇 |
2007年 | 30篇 |
2006年 | 12篇 |
2005年 | 19篇 |
2004年 | 53篇 |
2003年 | 41篇 |
2002年 | 57篇 |
2001年 | 46篇 |
2000年 | 22篇 |
1999年 | 47篇 |
1998年 | 40篇 |
1997年 | 25篇 |
1996年 | 11篇 |
1995年 | 12篇 |
1994年 | 10篇 |
1993年 | 9篇 |
1992年 | 3篇 |
1991年 | 8篇 |
1990年 | 6篇 |
1989年 | 2篇 |
1988年 | 3篇 |
1987年 | 3篇 |
1986年 | 1篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1979年 | 2篇 |
1977年 | 2篇 |
1975年 | 1篇 |
1974年 | 2篇 |
1969年 | 1篇 |
1968年 | 1篇 |
排序方式: 共有750条查询结果,搜索用时 292 毫秒
81.
H Yasuda T Mine H Shibata Y Eto Y Hasegawa T Takeuchi S Asano I Kojima 《Canadian Metallurgical Quarterly》1993,92(3):1491-1496
The present study was conducted to examine the effect of activin A on growth of rat hepatocytes. EGF induced a 10-fold increase in DNA synthesis as assessed by [3H]thymidine incorporation in cultured hepatocytes. When activin A was added together with EGF, DNA synthesis induced by EGF was markedly inhibited. Inhibition was detected at a concentration of 10(-10) M, and 5 x 10(-9) M activin A almost completely blocked EGF-mediated DNA synthesis. Similarly, activin A completely blocked DNA synthesis induced by hepatocyte growth factor/scatter factor. Activin A was capable of inhibiting EGF-mediated DNA synthesis, even when added 36 h after the addition of EGF. With the same time interval, TGF-beta also blocked EGF-induced DNA synthesis. Although both activin A and TGF-beta inhibited growth of hepatocytes in a similar manner, either activin A or TGF-beta did not compete with each other in their binding when assessed by competitive binding using an iodinated ligand. When hepatocytes were incubated with EGF, release of bioactivity of activin A into culture medium was detected after 48 h or later. Activity of activin A was released from parenchymal cells but not from nonparenchymal cells. mRNA for beta A subunit of activin was detected only slightly in unstimulated hepatocytes, but markedly increased at 48 h after the addition of EGF. To determine whether endogenously produced activin A affects DNA synthesis, we examined the effect of follistatin, an activin-binding protein that blocks the action of activin A. An addition of follistatin significantly enhanced EGF-induced DNA synthesis. Finally, in partial hepatectomized rat, expression of mRNA for beta A subunit in liver was markedly increased 24 h after the partial hepatectomy. These results indicate that activin A inhibits initiation of DNA synthesis in hepatocytes by acting on its own receptor and that activin A acts as an autocrine inhibitor of DNA synthesis in rat hepatocytes. 相似文献
82.
83.
84.
Taniguchi M Kato K Shimauchi A Ping X Nakayama H Fujita K Tanaka T Tarui Y Hirasawa E 《Journal of Bioscience and Bioengineering》2005,99(3):245-251
Cross-linked poly-gamma-glutamic acid (C-L gamma-PGA) markedly purified polluted water collected from rivers and ponds by flocculation and precipitation. This effect of C-L gamma-PGA occasionally required pretreatment with polyaluminum chloride (PAC). Components of polluted water in rivers or ponds are generally thought to be clay minerals, microorganisms and chemical compounds. In this study, the flocculating activities of C-L gamma-PGA against suspensions of bentonite, diatomaceous earth, Escherichia coli and Mycrocystis aeruginosa, and against solutions of crystal violet and bisphenol A were investigated. The mode of action of C-L gamma-PGA is thought to be based on electrostatic interaction between flocculants, C-L gamma-PGA and PAC, and the surface of polluted water components, which may lead to neutralization of the zeta-potential of those components. 相似文献
85.
Hayashi KG Matsui M Shimizu T Sudo N Sato A Shirasuna K Tetsuka M Kida K Schams D Miyamoto A 《Reproduction (Cambridge, England)》2008,136(6):787-797
We previously established a bovine experimental model showing that the corpus luteum (CL) does not appear following aspiration of the preovulatory follicle before the onset of LH surge. Using this model, the present study aimed to determine the profile of follicular development and the endocrinological environment in the absence of CL with variable nadir circulating progesterone (P(4)) concentrations during the oestrous cycle in cattle. Luteolysis was induced in heifers and cows and they were assigned either to have the dominant follicle aspirated (CL-absent) or ovulation induced (CL-present). Ultrasound scanning to observe the diameter of each follicle and blood collection was performed from the day of follicular aspiration or ovulation and continued for 6 days. The CL-absent cattle maintained nadir circulating P(4) throughout the experimental period and showed a similar diameter between the largest and second largest follicle, resulting in co-dominant follicles. Oestradiol (E(2)) concentrations were greater in the CL-absent cows than in the CL-present cows at day -1, day 1 and day 2 from follicular deviation. The CL-absent cows had a higher basal concentration, area under the curve (AUC), pulse amplitude and pulse frequency of LH than the CL-present cows. After follicular deviation, the CL-absent cows showed a greater basal concentration, AUC and pulse amplitude of growth hormone (GH) than the CL-present cows. These results suggest that the absence of CL accompanying nadir circulating P(4) induces an enhancement of LH pulses, which involves the growth of the co-dominant follicles. Our results also suggest that circulating levels of P(4) and E(2) affect pulsatile GH secretion in cattle. 相似文献
86.
Fatma Tosun Çiğdem Akyüz Kızılay Kevser Erol Fatma Sultan Kılıç Mine Kürkçüoğlu Kemal Hüsnü Can Başer 《Food chemistry》2008
The anticonvulsant activity of furanocoumarins, coumarin mixture and the essential oil obtained from the fruits of Heracleum crenatifolium was examined against maximal electroshock (MES)-induced seizures in mice. Bergapten showed significant anticonvulsant activity. The furanocoumarins isolated from the fruits of the plant were identified using thin-layer chromatography, melting points and spectroscopic methods (IR, MS, 1H NMR) as isobergapten (1), pimpinellin (2), bergapten (3), isopimpinellin (4), sphondin (5) and byak-angelicol (6). The essential oil content of the fruits were found as 5.5%. Twenty-two compounds representing 99.3% of the essential oil obtained from the fruits of H. crenatifolium were determined and the major components were identified as octanol and octyl acetate (3.1% and 88.4% respectively) by GC and GC–MS. 相似文献
87.
Inactivation of Penicillum expansum in sour cherry juice, peach and apricot nectars by pulsed electric fields 总被引:1,自引:0,他引:1
Inhibitory effects of pulsed electric fields (PEF) on Penicillum expansum inoculated into sour cherry juice, apricot and peach nectars were determined based on germination tube elongation, spore germination rate, and light and scanning electron microscopy (SEM) observations in this study. After inoculation of juice/nectar samples with P. expansum spores at the level of 10(5)-10(6)cfu/mL, the samples were processed by bench scale PEF pulse generator as a function of differing electric field strengths (0, 13, 17, 20, 23, 27, 30 and 34kV/cm) and processing times (0, 62, 94, 123, 163, 198 and 218mus). Results revealed that with an increase in electric field strength and processing time, germination tube elongation and spore germination rate were completely inhibited. Light and SEM observations revealed considerable morphological alterations in fungal conidia such as cytoplasmic coagulation, vacuolations, shrinkage and protoplast leakage. PEF processing of juice/nectars was demonstrated to be effective in inactivating P. expansum. To our knowledge, this is the first study confirming the inhibitory effects of PEF on germination tube elongation and spore germination rate of P. expansum in fruit juice/nectars. 相似文献
88.
89.
90.