Analysis of protein adsorption and binding at biosensor polymer interfaces using X-ray photon spectroscopy and scanning electrochemical microscopy

We describe a method, based on X-ray photoelectron spectroscopy (XPS) measurements, to assess the extent of protein adsorption or binding on a variety of different muTAS and biosensor interfaces. Underpinning this method is the labeling of protein molecules with either iodine- or bromine-containing motifs by using protocols previously developed for radiotracer studies. Using this method, we have examined the adsorption and binding properties of a variety of modified electrodeposited polymer interfaces as well as other materials used in muTAS device fabrication. Using polymer interfaces modified with poly(propylene glycol) (PPG) chains, our results indicate that a chain of at least approximately 30 monomer units is required to inhibit nonspecific adsorption from concentrated protein solutions. The XPS methodology was also used to probe specific binding of avidins and enzyme conjugates thereof to biotinylated and mixed biotin/PPG-modified polymer interfaces. In one example, using competitive binding, it was established that the mode of binding of a peroxidase-streptavidin conjugate to a biotinylated modified polymer interface was primarily via the streptavidin moiety (as opposed to nonspecific binding via the enzyme conjugate). XPS evaluation of nonspecific and specific peroxidase-streptavidin immobilization on various functionalized polymers has guided the design and fabrication of functionalized interdigitated electrodes in a biosensing muTAS device. Subsequent characterization of this device using scanning electrochemical microscopy (SECM) corroborated the adsorption and binding previously inferred from XPS measurements on macroscale electrodes.     

Psychological assessment of candidates for solid organ transplantation.

The role of psychologists, who can assist in preparing clients for transplant surgery and support them postsurgery to sustain the complex maintenance protocol, is outlined. An overview of specific procedures used in the psychological evaluation and treatment of solid organ transplant patients, a discussion of general issues involved in treating these patients, and case vignettes are provided. As transplants become more common, the need for additional psychologists as part of multidisciplinary treatment teams increases. Because many patients live great distances from the transplant center, community psychologists may be enlisted to treat organ transplant patients and to collaborate with psychologists on transplant teams. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Teacher ratings predict peer ratings of aggression at 3-year follow-up in boys with attention deficit disorder with hyperactivity.

Compared initial and 3-yr follow-up peer ratings of 19 boys, who were in Grades 1–3 at the beginning of the study, who had attention deficit disorder with hyperactivity (ADDH). When the entire group was considered, nominations on the Aggression and Likability factors of a pupil evaluation inventory improved. Subgroups were created using teacher ratings on an aggression scale. Peer ratings of Aggression improved for Ss whom teachers rated below the median on the aggression scale but not for Ss rated above the median. Subgroups reflecting other teacher and peer ratings were less effective in predicting differential change in peer ratings of ADDH Ss. (8 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The science and practice of empirically supported treatments.

The impact on Canadian professional psychological treatment practices of the American Psychological Association""s Division 12 (Clinical Psychology) Task Force""s development of criteria and listings for empirically supported psychological treatments, along with other industrial efforts to standardize the identification of treatments with established efficacy is described in the article by J. Hunsley et al (see record 1999-01869-001). Here Hunsley et al extend their previous discussion and reply to other commentaries (see records 1999-01869-002, 1999-01869-003, 1999-01869-004, 1999-01869-005, 1999-01869-006) by viewing the current emphases in the Canadian health care system on accountability and empirically supported treatment (ESTs) as an unparalleled opportunity for professional psychology to deliver on its birthright. The generalizability of US experiences to Canadian contexts is discussed. Clinical practice guidelines should and will become the norm for providing evidence-based services in psychology, yet it would be impossible to develop such a guideline without empirical evidence proving that there are "best" interventions for a given problem. Canadian research issues in this light are discussed along with future challenges to professional psychology in Canada. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The pigments of cottonseed

Summary A method for the purification of gossypol has been developed which yields material differing in optical properties from those previously reported for gossypol preparations. A colorimetric test for extractable gossypol has been described. It has been shown that cottonseed contains at least three pigments in addition to gossypol. Some of the properties of the three pigments have been reported. One of these newly detected pigments, gossypurpurin, has been shown to be the substance which, mixed with gossypol, constitutes the so-called “red gossypol” of Podol’skaja. The frequently reported water-dispersable blue pigment of cottonseed has been shown to be either a complex of gossypol, gossypurpurin and protein or a mixture of two protein-pigment complexes. Its dissociation into the two pigments and protein has been accomplished. Presented before the 34th Annual Meeting of the American Oil Chemists’ Society, New Orleans, Louisiana, May 12–14, 1943.     

Ontology-driven hypothesis generation to explain anomalous patient responses to treatment

Within the medical domain there are clear expectations as to how a patient should respond to treatments administered. When these responses are not observed it can be challenging for clinicians to understand the anomalous responses. The work reported here describes a tool which can detect anomalous patient responses to treatment and further suggest hypotheses to explain the anomaly. In order to develop this tool, we have undertaken a study to determine how Intensive Care Unit (ICU) clinicians identify anomalous patient responses; we then asked further clinicians to provide potential explanations for such anomalies. The high level reasoning deployed by the clinicians has been captured and generalised to form the procedural component of the ontology-driven tool. An evaluation has shown that the tool successfully reproduced the clinician’s hypotheses in the majority of cases. Finally, the paper concludes by describing planned extensions to this work.     

The Structured Assessment of Violence Risk in Youth as a predictor of recidivism in a United Kingdom cohort of adolescent offenders with conduct disorder.

This prospective study examined the predictive validity of the Structured Assessment of Violence Risk in Youth (SAVRY) and the Psychopathy Checklist: Youth Version (PCL: YV) in 99 male adolescents who were assessed in custody and followed up 12 months post release. Outcome data on recidivism were based on official Home Office records. The base rates for violent and general recidivism, respectively, were 38.4% and 70.7%. The predictive validity of the SAVRY Risk Total and the SAVRY Risk Rating was moderate for both violent and general recidivism, but both showed incremental validity in predicting outcomes compared with the PCL: YV. Data are discussed in relation to the limited published international literature. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Peer relations among adolescents with female same-sex parents.

This study examined associations among family type (same-sex vs. opposite-sex parents), adolescent gender, family and relationship variables, and the peer relations of adolescents. Participants included 44 adolescents parented by same-sex female couples and 44 adolescents parented by opposite-sex couples, matched on demographic characteristics and drawn from a national sample. On both self-reported and peer-reported measures of relations with peers, adolescents were functioning well, and the quality of their peer relations was not associated with family type. Regardless of family type, adolescents whose parents described closer relationships with them reported higher quality peer relations and more friends in school and were rated as more central in their friendship networks. (PsycINFO Database Record (c) 2010 APA, all rights reserved)