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71.
We have used proteolysis to examine the environment through which nascent secretory proteins are translocated across the membrane of the endoplasmic reticulum. After solubilization of rough microsomes with detergent, fragments comprised of the approximately 70 carboxyl-terminal amino acids of translocating nascent chains initiated and targeted in vivo were protected from digestion by added proteases. About 40 amino acids of nascent chains were protected from proteolysis by the ribosome; thus, membrane-derived components protect an additional 30 amino acids. Under conditions in which those 30 additional amino acids are protected, only a small set of integral membrane proteins remained associated with the ribosome. These proteins include the Sec61 complex previously identified as the core component of the membrane-bound protein translocation apparatus. These results support the concept of a translocation pore that makes intimate contact with the ribosome and thereby protects nascent chains from proteolytic digestion for an additional, constant length. 相似文献
72.
TheSpecial Issue on Applications of Temporal Models raises many issues of time: What are the important properties of time? How can time be best represented? How can one reason about time-dependent properties? What are the important directions of temporal research? This introductory piece very briefly surveys the current wide variety of temporal models, temporal reasoning methods, and applications to time-varying phenomena. Promising areas of investigation such as the verification of concurrent systems, knowledge-base representation methods, and dealing with theFrame Problem pass in fleeting review. Brief introductions to each of the works in the volume close the section. 相似文献
73.
Extending the Kernighan/Lin Heuristic for Hardware and Software Functional Partitioning 总被引:5,自引:0,他引:5
The Kernighan/Lin graph partitioning heuristic, also known as min-cut or group migration, has been extended over several decades very successfully for circuit partitioning. Those extensions customized the heuristic and its associated data structure to rapidly compute the minimum-cut metric central to circuit partitioning; as such, those extensions are not directly applicable to other problems. In this paper, we extend the heuristic for functional partitioning, which in turn can solve the much investigated codesign problem of partitioning a system's coarse-grained functions among hardware and software components. The key extension customizes the heuristic and data structure to rapidly compute execution-time and communication metrics, crucial to hardware and software partitioning, and leads to near-linear time-complexity and excellent resulting quality. Another extension uses a new criteria for terminating the heuristic, eliminating time-consuming and unnecessary fine-tuning of a partition. Our experiments demonstrate extremely fast execution times (just a few seconds) with results matched only by the slower simulated annealing heuristic, meaning that the extended Kernighan/Lin heuristic will likely prove hard to beat for hardware and software functional partitioning. 相似文献
74.
75.
76.
Amitava Roy Harvill C. Eaton Frank K. Cartledge Marty E. Tittlebaum 《Journal of hazardous materials》1992,30(3):297-316
The effect of sodium sulfate on the solidification/stabilization of a large quantity of a synthetic electroplating sludge in cementitious binders was s 相似文献
77.
78.
Frank Amoroso William W. Jones 《International Journal of Satellite Communications and Networking》1991,9(2):99-110
A geometric propagation model is developed for simulating the reception of direct sequence pseudonoise (DSPN) satellite signals by a directional or omni antenna in the dense scatterer mobile environment. The model is first validated for narrowband signals by a direct comparison of both simulated cumulative signal strength statistics and simulated diffuse Doppler spectra with classical theory. The model is then used to predict fading statistics for DSPN signaling, either with omni or directional antennas. As expected, the mitigation of fading is closely related to the ratio of DSPN chip duration to delay spread of the scatterer medium for both the omni and directional antennas. 相似文献
79.
Judiesch Michael K.; Schmidt Frank L.; Mount Michael K. 《Canadian Metallurgical Quarterly》1992,77(3):234
Examined distributions of estimates of the dollar value of performance in studies employing the method of F. L. Schmidt et al (1979) for estimating the standard deviation of job performance (SDy) and found evidence that (1) the mean 50th percentile estimate is biased downward, (2) estimates of SDy appear to be a constant percentage of the 50th percentile estimate, and (3) estimates of SDy as a percentage of the 50th percentile value (SDp) are quite similar to empirical SDp values based on actual employee output. These findings suggest that the downward bias in the mean estimate of the 50th percentile causes the mean estimates of SDy to be similarly biased downward, but does not bias the estimates of SDp. Finally, an objective method for estimating the value of average employee output is described. The product of this value and the mean supervisory estimate of SDp yields an unbiased estimate of SDy. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
80.
Tempest Philip R.; White Patricia; Williamson E.Diane; Titball Richard W.; Kelly David C.; Kemp Graham J.L.; Gray Peter M.D.; Forster Simon J.; Carr Frank J.; Harris William J. 《Protein engineering, design & selection : PEDS》1994,7(12):1501-1507
We have used the technique of antibody reshaping to producea humanized antibody specific for the a toxin of Clostridiumperfringens. The starting antibody was from a mouse hybridomafrom which variable (V) region nucleo-tide sequences were determined.The complementarity-determining regions (CDRs) from these Vregions were then inserted into human heavy and light chainV region genes with human constant region gene fragments subsequentlyadded. The insertion of CDRs alone into human frameworks didnot produce a functional reshaped antibody and modificationsto the V region framework were required. With minor frameworkmodifications, the affinity of the original murine mAb was restoredand even exceeded. Where affinity was increased, an alteredbinding profile to overlapping peptides was observed. Computermodelling of the reshaped heavy chain V regions suggested thatamino acids adjacent to CDRs can either contribute to, or distort,CDR loop conformation and must be adjusted to achieve high bindingaffinity. 相似文献