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排序方式: 共有807条查询结果,搜索用时 15 毫秒
61.
Hajime Eto 《Scientometrics》2000,48(1):85-97
This paper analyses communications between statistical methodology and applied statistics in terms of the similarity and dissimilarity in their authorship and citation patterns, and further the communication distance between them in terms of mutual citation and the time lag therein. Hypotheses are presented on their difference and distance and are verified for data from the Journal of the Royal Statistical Society, the oldest statistical society in the world. The data analysis reveals that they are indeed different and distant each other to a certain extent but less distinctly than initially conjectured in the hypotheses. 相似文献
62.
Liposome‐Based in Vitro Evolution of Aminoacyl‐tRNA Synthetase for Enhanced Pyrrolysine Derivative Incorporation 下载免费PDF全文
Dr. Atsuko Uyeda Dr. Takayoshi Watanabe Dr. Yasuhiko Kato Prof. Hajime Watanabe Prof. Tetsuya Yomo Prof. Takahiro Hohsaka Dr. Tomoaki Matsuura 《Chembiochem : a European journal of chemical biology》2015,16(12):1797-1802
Methanosarcina species pyrrolysyl‐tRNA synthetase (PylRS) attaches Pyl to its cognate amber suppressor tRNA. The introduction of two mutations (Y384F and Y306A) into PylRS was previously shown to generate a mutant, designated LysZ‐RS, that was able to attach N‐benzyloxycarbonyl‐L ‐lysine (LysZ) to its cognate tRNA. Despite the potential of LysZ derivatives, further LysZ‐RS engineering has not been performed; consequently, we aimed to generate LysZ‐RS mutants with improved LysZ incorporation activity through in vitro directed evolution. Using a liposome‐based in vitro compartmentalization (IVC) approach, we screened a randomly mutagenized gene library of LysZ‐RS and obtained a mutant that showed increased LysZ incorporation activity both in vitro and in vivo. The ease and high flexibility of liposome‐based IVC should enable the evolution of not only LysZ‐RS that can attach various LysZ derivatives but also of other enzymes involved in protein translation. 相似文献
63.
Microbial degradation of cyanobacterial cyclic peptides 总被引:1,自引:0,他引:1
Bacterial strain B-9 possesses hydrolytic enzymes capable of degrading microcystins (MCs) and nodularin that are toxic cyclic peptides produced by cyanobacteria. In the present study, the degradation activities of the cell extract of B-9 against non-toxic cyanobacterial cyclic peptides other than the MCs and nodularin were investigated, and the degradation products were analyzed by liquid chromatography/ion trap tandem mass spectrometry (LC/ITMS). It was confirmed that B-9 could also degrade these cyanobacterial cyclic peptides by hydrolysis of their peptide bonds. These results indicated that this bacterium possesses a very unique hydrolytic activity that can degrade structurally different cyclic peptides and that this may be effective for the detoxification of hazardous cyclic peptides. 相似文献
64.
Kubota K Kasuga F Iwasaki E Inagaki S Sakurai Y Komatsu M Toyofuku H Angulo FJ Scallan E Morikawa K 《Journal of food protection》2011,74(10):1592-1598
Most cases of acute gastroenteritis and foodborne disease are not ascertained by public health surveillance because the ill person does not always seek medical care and submit a stool sample for testing, and the laboratory does not always test for or identify the causative organism. We estimated the total burden of acute gastroenteritis in Miyagi Prefecture, Japan, using data from two 2-week cross-sectional, population-based telephone surveys conducted in 2006 and 2007. To estimate the number of acute gastroenteritis illnesses caused by Campylobacter, Salmonella, and Vibrio parahaemolyticus in Miyagi Prefecture, we determined the number of cases for each pathogen from active laboratory-based surveillance during 2005 to 2006 and adjusted for seeking of medical care and submission of stool specimens by using data from the population-based telephone surveys. Monte Carlo simulation was used to incorporate uncertainty. The prevalence of acute gastroenteritis in the preceding 4 weeks was 3.3% (70 of 2,126) and 3.5% (74 of 2,121) in the winter and summer months, yielding an estimated 44,200 episodes of acute gastroenteritis each year in this region. Among people with acute gastroenteritis, the physician consultation rate was 32.0%, and 10.9% of persons who sought care submitted a stool sample. The estimated numbers of Campylobacter-, Salmonella-, and V. parahaemolyticus -associated episodes of acute gastroenteritis were 1,512, 209, and 100 per 100,000 population per year, respectively, in this region. These estimates are significantly higher than the number of reported cases in surveillance in this region. Cases ascertained from active surveillance were also underrepresented in the present passive surveillance, suggesting that complementary surveillance systems, such as laboratory-based active surveillance in sentinel sites, are needed to monitor food safety in Japan. 相似文献
65.
Kunihisa Iwai Akio Onodera Hajime Matsue 《Journal of the science of food and agriculture》2003,83(15):1593-1599
The fruit of Viburnum dilatatum Thunb (gamazumi) has been shown to prevent oxidative injury induced by water immersion restraint stress (WIRS) in rats. In this study the effect of gamazumi crude extract (GCE) on antioxidant enzymatic activities in the plasma, liver and stomach of rats with WIRS was investigated to elucidate the mechanism of prevention of oxidative injury. Ulcer formation and lipid peroxidation were inhibited in rats supplied GCE for 2 weeks in comparison with rats supplied water. Although the activities of plasma, hepatic and gastric antioxidant enzymes in rats given water were decreased by WIRS, only slight changes were observed in rats given GCE. There was no difference in enzymatic activities between the water and GCE groups not subjected to WIRS. Furthermore, ferrous ascorbate‐induced oxidation in hepatic homogenate from rats given GCE was inhibited. These results suggest that ingestion of GCE does not induce antioxidant enzymes and that the absorbed antioxidant components of GCE have a direct effect on oxidative injury in the body. Copyright © 2003 Society of Chemical Industry 相似文献
66.
67.
Hajime Araki Taichi Kaji Masahito Yamamoto Keiji Suzuki Azuma Ohuchi 《Electrical Engineering in Japan》2001,135(4):43-51
The optimal sequential problem is defined as the problem of finding the minimum cost partition of the nodes of a directed acyclic graph into subsets of a given size, subject to the constraint that the precedence relationships among the elements are satisfied. A heuristic algorithm based on tabu search has been proposed for this problem [2]. However, there is a tendency for the solutions obtained by tabu search to become trapped in bad local optima in parallel graphs with random edge costs. In this paper we present a genetic algorithm for the optimal sequential partitioning problem. We develop an effective two‐point partial order crossover satisfying sequential conditions, which preserve better blocks that have a larger sum of edge costs. In this crossover we introduce the roulette selection method to escape local optima. We also assess the effectiveness of the algorithm. The results show that this proposed algorithm outperforms any other algorithm using tabu search in terms of solution quality. © 2001 Scripta Technica, Electr Eng Jpn, 135(4): 43–51, 2001 相似文献
68.
Hajime Yano Daigo Kouro Naoki Sasaki Shin-ichi Muramatsu 《Solar Energy Materials & Solar Cells》2009,93(6-7):976-979
We have found that the short-circuit current, Jsc, of polymer/fullerene [RR-P3HT/C60] solar cells has a clear dependence on the surface roughness of the ITO/glass substrate. We prepared an ITO surface with an average roughness, Ra, of 0.7–11 nm by chemical etching. At first Jsc increases with the increase in ITO surface roughness and then gradually decreases. The maximum performance was obtained at Ra≈4 nm. Jsc is also high with a very flat surface of Ra=0.7 nm. This feature can be attributed to the trade-off between the increase in absorption light path length and film-quality deterioration. 相似文献
69.
70.
Shiro Seki Takeshi Kobayashi Nobuyuki Serizawa Yo Kobayashi Katsuhito Takei Hajime Miyashiro Kikuko Hayamizu Seiji Tsuzuki Takushi Mitsugi Yasuhiro Umebayashi Masayoshi Watanabe 《Journal of power sources》2010,195(18):6207-6211
The physicochemical and electrochemical properties of three 1-alkyl-2,3,5-trimethylpyrazolium cation-based room-temperature ionic liquids with various alkyl chain lengths were investigated. The temperature dependences of density, viscosity, and ionic conductivity were obtained by precise measurements. Electrolyte properties of these room-temperature ionic liquids were also examined from the viewpoint of their uses in lithium secondary batteries ([LiCoO2 positive electrode|electrolyte|lithium metal negative electrode]). It was found that the alkyl chain length affects the charge–discharge performances of cells. 相似文献