Morphological evaluation of bioartificial hydrogels as potential tissue engineering scaffolds

Poly(vinyl alcohol) hydrogels prepared by freeze-thawing procedure represent synthetic systems widely investigated as non-biodegradable scaffolds for tissue regeneration. In order to improve the biocompatibility properties of pure poly(vinyl alcohol) (PVA) hydrogels, blends of PVA with different biological macromolecules, such hyaluronic acid, dextran, and gelatin were prepared and used to produce bioartificial hydrogels. The porosity characteristics of these hydrogels were investigated by scanning electron microscopy and mercury intrusion porosimetry. The morphology of bioartificial hydrogels was evaluated and compared with that of pure PVA hydrogels. In particular the effect exerted by each biological component on pore size and distribution was investigated. The obtained results indicate that when a natural macromolecule is added to PVA the internal structure of the material changes. A small amount of biopolymer induces the structural elements of PVA matrix to take on a well evident lamellar appearance and an apparent preferential orientation. Comparing the results of SEM and mercury intrusion porosimetry it was concluded that hydrogels containing 20% of biological component have the most regular structure and at the same time the lowest total porosity. On the contrary samples with the highest content of natural polymer (40%) show the less regular structure and the highest total porosity.     

Model-Reference Fuzzy Adaptive Control as a Framework for Nonlinear System Control

The paper presents a general methodology of adaptive control based on fuzzy model to deal with unknown plants. The problem of parameter estimation is solved using a direct approach, i.e. the controller parameters are adapted without explicitly estimating plant parameters. Thus, very simple adaptive and control laws are obtained using Lyapunov stability criterion. The generality of the approach is substantiated by Stone-Weierstrass theorem, which indicates that any continuous function can be approximated by fuzzy basis function expansion. In the sense of adaptive control, this implies the adaptive law with fuzzified adaptive control parameters. The proposed control algorithm may be viewed as an extension of classical adaptive control for linear plants, but compared to the latter it provides higher adaptation ability and consequently better performance if the plant is nonlinear. The global stability of the control system is assured and the tracking error converges to the residual set that depends on fuzzification properties. The main advantage of the approach is simplicity that suits control engineers since wide range of industrial processes can be controlled by the proposed method. In the paper, the control of heat exchanger is performed.     

The raison d'être of constitutively active protein kinases: the lesson of CK2

Protein kinases generally are tightly controlled signaling molecules that are switched on only in response to specific stimuli. Exceptionally few protein kinases are constitutively active, the most striking example being provided by CK2 (formerly "casein kinase 2"). Owing to unique structural features, the catalytic activity of CK2 is constantly on, although its targeting can be deeply influenced by the association of its two catalytic subunits (alpha and/or alpha') with a dimeric non catalytic beta subunit. Constitutive activity of CK2 reflects its extraordinary pleiotropy documented by its growing list of >300 protein substrates and is consistent with emerging evidence that CK2 plays an essential role in the cell by counteracting premature and/or unscheduled apoptosis, thus ensuring cell survival under stress conditions.     

Changes in selected biochemical and sensory parameters as affected by the superficial inoculation of Penicillium camemberti on dry fermented sausages

An atoxigenic strain of Penicillium camemberti was superficially inoculated on fermented sausages in an attempt to improve their sensory properties. The growth of this mould on the surface of the sausages resulted in an intense proteolysis and lipolysis, which caused an increase in the concentration of free amino acids, free fatty acids (FFA) and volatile compounds. Many of these were derived from amino acid catabolism and were responsible for the "ripened flavour", i.e. branched aldehydes and the corresponding alcohols, acids and esters. The development of the fungal mycelia on the surface of the sausages also protected lipids from oxidation, resulting in both lower 2-thiobarbituric acid (TBARS) values and lipid oxidation-derived compounds, such as aliphatic aldehydes and alcohols. The sensory analysis of superficially inoculated sausages showed clear improvements in odour and flavour and, as a consequence, in the overall quality of the sausages. Therefore, this strain is proposed as a potential starter culture for dry fermented sausage production.     

An Integrated CMOS Front-End for Optical Absolute Rotary Encoders

This paper presents a front-end circuit for optical rotary encoders. The light pulses modulated by the encoder disc are transduced into current signals, which are pre-processed and converted into digital waveforms related to the disc angular position information. The proposed front-end circuit is compensated against temperature drifts. Digitally programmable calibration is provided to account for spreads in impinging light pulse power. Measurement results on integrated prototypes are shown, demonstrating correct operation of the front-end with an optical input power from 0.5 W to 3 W up to a signal frequency of 500 kHz in a temperature range from 0 °C to 80 °C.     

Optimisation of the throttling system in a CO2 refrigerating machine

This paper is an answer to the need of finding the optimal solution for the throttling system in refrigerating machines using CO₂ as working fluid; such a solution must combine reliability, low installation cost and high energy efficiency. To this purpose, different expansion systems are compared by means of a simulation programme, including a new one, operating with a differential valve, a liquid receiver and a thermostatic valve. The typical compression refrigerating cycle performed by CO₂ involves transcritical operations and therefore the upper pressure needs to be adjusted to the optimal value, that, unlike the traditional cycle, is not determined by heat transfer. The innovative system here proposed shows an intrinsic self-adjusting capability that leads to COP values quite close to the maximum ones when a fixed suitable value of the differential pressure is chosen, even if the temperature of the secondary fluid varies to a large extent.     

Squaring down LTI systems: A geometric approach

In this paper, the problem of reducing a given LTI system into a left or right invertible one is addressed and solved with the standard tools of the geometric control theory. First, it will be shown how an LTI system can be turned into a left invertible system, thus preserving key system properties like stabilizability, phase minimality, right invertibility, relative degree and infinite zero structure. Moreover, the additional invariant zeros introduced in the left invertible system thus obtained can be arbitrarily assigned in the complex plane. By duality, the scheme of a right inverter will be derived straightforwardly. Moreover, the squaring down problem will be addressed. In fact, when the left and right reduction procedures are applied together, a system with an unequal number of inputs and outputs is turned into a square and invertible system. Furthermore, as an example it will be shown how these techniques may be employed to weaken the standard assumption of left invertibility of the plant in many optimization problems.     

Simulation of the St. Francis Dam-Break Flood

A two-dimensional (2D) simulation of flooding from the 1928 failure of St. Francis Dam in southern California is presented. The simulation algorithm solves shallow-water equations using a robust unstructured grid Godunov-type scheme designed for wetting and drying and achieves good results. Flood extent and flood travel time are predicted within 4 and 10% of observations, respectively. Representation of terrain by the mesh is identified as the dominant factor affecting accuracy, and an iterative process of mesh refinement and convergence checks is implemented to minimize errors. The most accurate predictions are achieved with a uniformly distributed Manning n = 0.02. A 50% increase in n increases travel time errors to 25% but has little effect on flood extent predictions. This highlights the challenge of a priori travel time prediction but robustness in flood extent prediction when topography is well resolved. Predictions show a combination of subcritical and supercritical flow regimes. The leading edge of the flood was supercritical in San Francisquito Canyon, but due to channel tortuosity, the wetting front reflected off canyon walls causing a transition to subcritical flow, considerably larger depths, and a standing wave in one particular reach that accounts for a 30% fluctuation in discharge. Elsewhere, oblique shocks locally increased flood depths. The 2D dam-break model is validated by its stability and accuracy, conservation properties, ability to calibrate with a physically realistic and simple resistance parametrization, and modest computational cost. Further, this study highlights the importance of a dynamic momentum balance for dam-break flood simulation.     

Reconfigurable digital controller for a buck converter based on FPGA

This paper presents a complete digitally controlled dc–dc buck converter performed by FPGA circuitry. All tasks, analog to digital conversion, control algorithm and pulse width modulation, were implemented in the FPGA. This approach enables high-speed dynamic response and programmability by the controller, without external passive components. In addition, the controller’s structure can be easily changed without external components. The applied algorithm enables a switching frequency of 100 kHz.     

Skew product cycles with rich dynamics: From totally non-hyperbolic dynamics to fully prevalent hyperbolicity

We introduce a two-parameter family of ‘partially hyperbolic’ skew products (G_{a, t})_{a > 0, t ∈ [ ? ε, ε]} maps with one dimensional centre direction. In this family, the parameter a models the central dynamics and the parameter t the unfolding of cycles (that occurs for t = 0). The parameter a also measures the ‘central distortion’ of the systems: for small a, the distortion of the systems is small and it increases and goes to infinity as a → ∞. The family (G_{a, t}) displays some of the main characteristic properties of the unfolding of heterodimensional cycles as intermingled homoclinic classes of different indices and secondary bifurcations via collision of hyperbolic homoclinic classes. For a ∈ (0, log?2), the dynamics of (G_{a, t}) is always non-hyperbolic after the unfolding of the cycle. However, for a > log?4 intervals of t-parameters corresponding to hyperbolic dynamics appear and turn into totally prevalent as a → ∞ (the density of ‘hyperbolic parameters’ goes to 1 as a → ∞). The dynamics of the maps G_{a, t} is described using a family of iterated function systems modelling the dynamics in the one-dimensional central direction.