The effect of different pollutants exposure on the pigment content of pigmented macrophage aggregates in the spleen of Vardar chub (Squalius vardarensis Karaman, 1928)

Pigmented macrophage aggregates (MAs) are known to change under influence of various factors, such as aging, season, starvation, and/or pollution. In this study, changes in the pigment content of the MAs in the spleen of Vardar chub (Squalius vardarensis, Karaman) (n = 129) collected in spring and autumn, from three rivers with different pollution impact was examined: Zletovska River (metals), Kriva River (metals and municipal wastewater), and Bregalnica River (municipal wastewater). Collected data revealed increased relative volume and number of MAs containing hemosiderin under the influence of metals, significant in autumn (p < .05). In chub exposed to metals combined with municipal wastewater, significant increase of lipochrome accumulation in MAs in autumn, melanin in MAs in fish captured in the spring season, and number of splenic MAs containing combination of melanin and lipochrome was noted. Volumes and number of MAs containing combination of hemosiderin and lipochrome increased in spleen of fish captured in autumn from both Zletovska River and Kriva River, most likely due to the contribution of hemosiderin and lipochrome, respectively. Values measured for the various pigments in splenic MAs in fish captured from Bregalnica River, were overall closer to the values measured for fish captured from Kriva River. Notably, melanin and lipochrome are more likely to be found in fish from waters influenced by municipal wastewater (organic pollution) and hemosiderin in fish spleen from water influenced by mining activity (heavy metals pollution).     

Towards Dissecting the Mechanism of Protein Phosphatase-1 Inhibition by Its C-Terminal Phosphorylation

Phosphoprotein phosphatase-1 (PP1) is a key player in the regulation of phospho-serine (pSer) and phospho-threonine (pThr) dephosphorylation and is involved in a large fraction of cellular signaling pathways. Aberrant activity of PP1 has been linked to many diseases, including cancer and heart failure. Besides a well-established activity control by regulatory proteins, an inhibitory function for phosphorylation (p) of a Thr residue in the C-terminal intrinsically disordered tail of PP1 has been demonstrated. The associated phenotype of cell-cycle arrest was repeatedly proposed to be due to autoinhibition of PP1 through either conformational changes or substrate competition. Here, we use PP1 variants created by mutations and protein semisynthesis to differentiate between these hypotheses. Our data support the hypothesis that pThr exerts its inhibitory function by mediating protein complex formation rather than by a direct mechanism of structural changes or substrate competition.     

In situ study of electric-field-induced ferroelectric and antiferromagnetic domain switching in polycrystalline BiFeO3

Antiferromagnetic domain switching induced by ferroelectric polarization switching has previously been observed in situ in both multiferroic BiFeO₃ single crystals and thin films. Despite a number of reports on macroscopic magnetoelectric measurements on polycrystalline BiFeO₃, direct in situ observation of electric-field-induced antiferromagnetic domain switching in this material has not been addressed due to the lack of high-quality samples capable of electrical poling. Here, the electric field control of antiferromagnetic domain texture is identified in polycrystalline BiFeO₃ using in situ neutron diffraction, showing the resultant magnetic domain reorientation induced by an electric field. An antiferromagnetic domain reorientation to a value of 2.2-2.5 multiples of a random distribution (MRD) is found to be induced by an electric field that provides a non-180° ferroelectric-ferroelastic domain texture of 2.2-2.5 MRD along the field direction. The current results show well-controlled coupling of multiferroic domain texturing in single-phase polycrystalline BiFeO₃.     

Issues and approaches in the design of collective autonomous agents

The problem of synthesizing and analyzing collective autonomous agents has only recently begun to be practically studied by the robotics community. This paper overviews the most prominent directions of research, defines key terms, and summarizes the main issues. Finally, it briefly describes our approach to controlling group behavior and its relation to the field as a whole.     

Automated Derivation of Primitives for Movement Classification

We describe a new method for representing human movement compactly, in terms of a linear super-imposition of simpler movements termed primitives. This method is a part of a larger research project aimed at modeling motor control and imitation using the notion of perceptuo-motor primitives, a basis set of coupled perceptual and motor routines. In our model, the perceptual system is biased by the set of motor behaviors the agent can execute. Thus, an agent can automatically classify observed movements into its executable repertoire. In this paper, we describe a method for automatically deriving a set of primitives directly from human movement data.We used movement data gathered from a psychophysical experiment on human imitation to derive the primitives. The data were first filtered, then segmented, and principal component analysis was applied to the segments. The eigenvectors corresponding to a few of the highest eigenvalues provide us with a basis set of primitives. These are used, through superposition and sequencing, to reconstruct the training movements as well as novel ones. The validation of the method was performed on a humanoid simulation with physical dynamics. The effectiveness of the motion reconstruction was measured through an error metric. We also explored and evaluated a technique of clustering in the space of primitives for generating controllers for executing frequently used movements.     

Tablet game-supported speech therapy embedded in children’s popular practices

Modern speech-language pathology practices (SLPPs) have adopted tablet games in recent years. Research is needed to take advantage of the potential benefits tablets can offer and to explore the factors that influence its introduction. This paper discusses a survey that was conducted to investigate the factors that influence speech-language pathologists’ (SLPs) beliefs and the actual use of tablets in SLPPs. The results of the survey, conducted among Slovenian SLPs, indicated that their most positive beliefs concerning tablets relate to social influence. Specifically, the beliefs relate to children’s interest and practice with tablets and have a significant impact on the decision-making of SLPs concerning introducing tablets in therapy. Conversely, SLPs’ most negative beliefs relate to the conditions for facilitating tablet use, particularly the lack of Slovenian applications. This paper discusses a separate case study that was conducted in which tablet game-supported therapy was introduced to a group of 44 children. The case study results indicated that the children accepted the use of tablet games for therapy purposes and the games had a positive impact on the children’s motivation and satisfaction.     

Evaluating fault tolerance approaches in multi-agent systems

A multi-agent system (MAS) is a distributed system that consists of multiple agents working together to solve mutual problems. Even though MASs are well suited for the development of complex distributed systems, the number of real-world usages is still small. One of the main reasons for this is that MASs are very fragile. In a typical, large-scale MAS, the rate of failure grows with the number of hosts, the number of deployed agents, and the duration of the agent’s task execution. For this reason, numerous approaches have been introduced to deal with aspects of failure handling. However, the absence of centralized control and a large number of individual intelligent components makes it difficult to detect and treat errors. The risk of uncontrollable fault propagation is high and can seriously impact on system performance. There are two important factors that limit the usage of MASs: (1) existing fault tolerance (FT) approaches are not generic, as they focus on and improve specific issues of FT; and (2) despite the plethora of available FT approaches and theories, there is a remarkable lack of general metrics, tools, benchmarks, and experimental methods for formal validation and comparison of existing or newly developed FT approaches. As FT approaches in MASs become a well-established field, the need for generalized, standardized evaluation of FT approaches emerges as imperative. In this paper, we first present a detailed overview of existing FT solutions, approaches, and techniques in agent platform hosted MASs. From that overview, we derive the commonalities in existing research. Next, we present the main contribution of our paper: an evaluation methodology, with a set of metrics, for comparing FT approaches in MASs. We adopt an engineering perspective on the problem, defining a methodology and metrics that are both implementation- and domain-independent. The metrics are formalized with an acyclic directed graph. By using our methodology, evaluators can select an appropriate FT approach for targeted MAS application, thus improving MAS usability, stability, and development speed. In order to show the viability of our approach, a case study that compares two FT approaches for a targeted MAS is presented. The case study results show that our methodology can be used for selecting an appropriate FT approach for the targeted MAS.     

Cycling of electrodeposited beta lead dioxide

The electrochemical behaviour of lead dioxide cycled in sulphuric acid under galvanostatic (discharge) and potentiostatic (charge) conditions has been investigated. The results illustrate the effect of the sulphate barrier layer forming on PbO₂. This layer shifts the equilibrium potential of the electrodes without influence on the kinetics of the reduction, which seems to be dependent on a diffusive step associated with the onset of an O^2– gradient in the dioxide. As for the sulphate oxidation, it has been found that this process is mainly affected by the end potential reached during the previous galvanostatic reduction.     

PtRu Colloid Nanoparticles for CO Oxidation in Microfabricated Reactors

The catalytic activity of PtRu colloid nanoparticles for CO oxidation is investigated in microfabricated reactors. The measured catalytic performance describes a volcano curve as a function of the Pt/Ru ratio. The apparent activation energies for the different alloy catalysts are between 21 and 117 kJ/mol, which agree well with literature. The size distribution of the colloid nanoparticles is determined from STM and TEM, from which an average size of the colloid nanoparticles of 2.2±0.5 nm is determined.