Production of extracellular bifidogenic growth stimulator (BGS) from Propionibacterium shermanii using a bioreactor system with a microfiltration module and an on-line controller for lactic acid concentration

Production of a bifidogenic growth stimulator (BGS) by Propionibacterium freudenreichii subsp. shermanii (Propionibacterium shermanii) using lactic acid as a carbon source was investigated using different cultivation methods. When a continuous bioreactor system with a filtration device was used at a dilution rate of 0.075 h(-1), the average BGS concentration was 2.4 mg/l, which corresponds to a BGS productivity per cultivation time of 1.8 x 10(-1) mg x l(-1) x h(-1). The BGS productivity per cultivation time in continuous cultivation with filtration was 1.9-fold that (9.4 x 10(-2) mg x l(-1).h(-1)) in a conventional batch cultivation. In fed-batch cultivation with feed-back control using an on-line lactic acid controller with a lactic acid biosensor, it was possible to prevent substrate inhibition by maintaining the lactic acid concentration in culture broth low at 3.3 g/l, and an enhanced BGS production (31 mg/l) was successfully attained. The BGS productivity per cultivation time (2.1x10(-1) mg x l(-1) x h(-1)) in the fed-batch cultivation with feed-back control was 2.2-fold that in the conventional batch cultivation. A new bioreactor system was developed by coupling a continuous bioreactor system with a filtration device to an on-line lactic acid controller. Using the new bioreactor system, we produced BGS continuously at a high level of 47 mg/l. The BGS productivities per cultivation time (3.5 mg.l(-1) x h(-1)) and the total volume of medium used (1.7 x 10(-1) mg x l(-1) x h(-1)) obtained in the new bioreactor system were 37-fold and 2.1-fold those in the conventional batch cultivation, respectively. These results described above clearly demonstrate the positive effects of both the continuous filtration for removal of metabolites (propionic and acetic acids) inhibitory to cell growth and feed-back control of lactic acid concentration in the culture broth on BGS production by P. shermanii. This paper is the first report on BGS production by the propionic acid bacterium using lactic acid as a carbon source.     

Synthesis and degradation of acyl peptide using enzyme from Pseudomonas aeruginosa

The detailed properties of the enzyme from Pseudomonas aeruginosa, which catalyzes the N-acyl linkage between myristic acid and the N-terminal glycine residue of the octapeptide GNAAAARR-NH(2) (PKA) in aqueous solution without ATP and CoA, were studied. The substrate specificity for the acyl peptide in the synthetic reaction was examined, and it was found that at least eight amino acid residues are required for the reaction and that the N-terminal glycine residue is not absolutely essential for the reaction because the activity was detected using the octapeptide that has an N-terminal alanine. The activity was also strongly affected by the amino acid sequence because the activity was very weak in the reaction using GARASVLS-NH(2) (HIV-1p17(gag)). The substrate specificity for fatty acids was also examined. In the reactions using lauric acid and decanoic acid, only slight activities were detected; however, those activities were very small compared with the activity in the reaction using myristic acid. In addition, the degradation of myristoyl PKA by the enzyme was detected, although there are only a few reports on demyristoylation. The optimum pH and temperature of the degradation reaction were consistent with those of the synthetic reaction. The degradation reaction was inhibited by divalent cations.     

Liquefaction Resistance of Sandy Soils under Partially Drained Condition

In order to simulate the effect of drainage on soils adjacent to gravel drains that are installed as countermeasure against liquefaction, several series of cyclic triaxial tests were performed on saturated sands under partially drained conditions. The condition of partial drainage under cyclic loading was simulated in the laboratory using triaxial testing equipment installed with a drainage control valve to precisely regulate the volume of water being drained from test specimens. Effects of both drainage conditions and loading frequencies on cyclic response were incorporated through the coefficient of drainage effect, α*. Experimental results showed that for sand exhibiting strain softening, the partially drained response was controlled by the critical effective stress ratio while for sand showing strain hardening behavior, the controlling factor was the phase transformation stress ratio. Moreover, test results indicated that the minimum liquefaction resistance under partially drained conditions can be used as a parameter to describe the liquefaction resistance of sands improved by the gravel drain method. From these results, a simplified procedure for designing gravel drains based on the factor of safety (FL) concept was proposed.     

Behaviour of piezoelectric lead zirconate titanate irradiating with high-energy xenon ions

The characteristics of a detector fabricated using piezoelectric lead zirconate titanate (PZT) were studied by irradiating it with a 400 MeV/n xenon (Xe) beam while changing the beam flux intensity. The largest output signal was observed from the detector when the beam power per unit time was estimated to be largest. It was also found that the sensitivity per Xe ion of the detector seemed to be higher with decreasing the amount of the Xe ion flux within the limits of the experimental conditions.     

Effect of Alloying Elements on Microstructure,Martensitic Transformation and Mechanical Properties of Ni-Mn Based Alloys

The microstructural features,shape memory behavior and mechanical properties of Ni-Mn based alloys were investigated by dierential scanning calorimetry(DSC),scanning electron microscopy(SEM),transmission electron microscopy(TEM)and thermal cycling test under various stresses.The transformation temperatures shifted toward lower temperatures when adding a third element into the Ni-Mn system.The addition of 10 at. pct Fe increased considerably the mechanical properties exhibiting still high transformation temp...     

Establishment of Intestinal Organoid from Rousettus leschenaultii and the Susceptibility to Bat-Associated Viruses,SARS-CoV-2 and Pteropine Orthoreovirus

Various pathogens, such as Ebola virus, Marburg virus, Nipah virus, Hendra virus, Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV), Middle East Respiratory Syndrome Coronavirus (MERS-CoV), and SARS-CoV-2, are threatening human health worldwide. The natural hosts of these pathogens are thought to be bats. The rousette bat, a megabat, is thought to be a natural reservoir of filoviruses, including Ebola and Marburg viruses. Additionally, the rousette bat showed a transient infection in the experimental inoculation of SARS-CoV-2. In the current study, we established and characterized intestinal organoids from Leschenault’s rousette, Rousettus leschenaultii. The established organoids successfully recapitulated the characteristics of intestinal epithelial structure and morphology, and the appropriate supplements necessary for long-term stable culture were identified. The organoid showed susceptibility to Pteropine orthoreovirus (PRV) but not to SARS-CoV-2 in experimental inoculation. This is the first report of the establishment of an expandable organoid culture system of the rousette bat intestinal organoid and its sensitivity to bat-associated viruses, PRV and SARS-CoV-2. This organoid is a useful tool for the elucidation of tolerance mechanisms of the emerging rousette bat-associated viruses such as Ebola and Marburg virus.     

Bias Removal Algorithm for 2-D Equation Error Adaptive IIR Filters

This paper proposes a bias removal algorithm for equation error-based 2-D adaptive cascade IIR filters with separable denominator function. As well known, equation error-based adaptive IIR filtering algorithms have the advantages of fast convergence and unimodal mean-square-error surface. These advantages, however, come along with the drawback of biased parameter estimates in the presence of measurement noise. The adaptive filter structure in the proposed algorithm is based on the concept of backpropagating the desired signal through a cascade of the denominator vertical and horizontal sections. To handle the bias problem, the proposed algorithm uses a scaled value of the output error of each of the cascaded sections as an estimate for the measurement noise embedded in the signal part of the coefficient-update procedure of that section. Thus, while maintaining the advantages of easy stability monitoring, fast convergence, and low computational load, the effect of the measurement noise is suppressed. Input-Output stability analysis is carried out, and the constraints required to maintain stability are derived. Simulation examples are presented to support the effectiveness and the usability of the proposed bias removal algorithm in 2-D system identification and image enhancement applications.     

A 2.2-V operation, 2.4-GHz single-chip GaAs MMIC transceiver forwireless applications

A 2.2-V operation, single-chip GaAs MMIC transceiver has been successfully developed for 2.4-GHz-band wireless applications such as wireless local area network terminals. The chip is fabricated using a planar self-aligned gate field-effect transistor. To generate sufficient negative voltage for gate-biasing and to enhance switch power handling capability under a 2.2-V supply, a newly designed negative voltage generator with a voltage doubler (NVG-VD) and a switch control logic circuit are integrated on the chip, together with a power amplifier, a transmit/receive switch, and a low-noise amplifier. The NVG-VD is designed to produce both a 3.3-V positive step-up voltage and a -2.1-V negative voltage under 2.2 V in operation voltage. Biased with these outputs, the logic circuit accommodates high power outputs of over 25 dBm with a low operating voltage of 2.2 V in transmit mode, With a 2.45-GHz modulated signal based on IS-95 standards, a 21-dBm output power and a 33% efficiency are obtained at a ±1.25-MHz-offset adjacent channel power rejection of -45 dBc. In receive mode, a low-noise amplifier achieves a 1.8-dB noise figure and an 11-dB gain with a 3.0-mA current. This transceiver enables significant size and weight reductions in 2.4-GHz-band wireless application terminals     

The Role of RASGRP2 in Vascular Endothelial Cells—A Mini Review

RAS guanyl nucleotide-releasing proteins (RASGRPs) are important proteins that act as guanine nucleotide exchange factors, which activate small GTPases and function as molecular switches for intracellular signals. The RASGRP family is composed of RASGRP1–4 proteins and activates the small GTPases, RAS and RAP. Among them, RASGRP2 has different characteristics from other RASGRPs in that it targets small GTPases and its localizations are different. Many studies related to RASGRP2 have been reported in cells of the blood cell lineage. Furthermore, RASGRP2 has also been reported to be associated with Huntington’s disease, tumors, and rheumatoid arthritis. In addition, we also recently reported RASGRP2 expression in vascular endothelial cells, and clarified the involvement of xenopus Rasgrp2 in the vasculogenesis process and multiple signaling pathways of RASGRP2 in human vascular endothelial cells with stable expression of RASGRP2. Therefore, this article outlines the existing knowledge of RASGRP2 and focuses on its expression and role in vascular endothelial cells, and suggests that RASGRP2 functions as a protective factor for maintaining healthy blood vessels.     

Permanent magnet synchronous motors using primarily reluctance torque

Permanent magnet synchronous motors (PMSMs) are widely used in many applications as high‐performance variable‐speed drives. In traction drives, such as those used for electric vehicles or for the compressor drives of air conditioners, a wide constant‐power speed range and high‐efficiency operation are desired. The aim of this paper is to develop a high‐performance PMSM that offers high‐efficiency performance in the high‐speed region, including light‐load conditions, as well as a wide constant‐power speed range. Simulations show that the proposed interior PMSM, which produces chiefly reluctance torque and in which the permanent magnet flux assists torque production, is capable of meeting the above performance requirements. A prototype PMSM is designed in accordance with this new design concept and several drive tests are carried out. The saliency ratio of the prototype IPMSM is about 5, and, as a result, reluctance torque is the principal torque component, representing more than 70% of total torque. The prototype IPMSM can attain a constant‐power speed range of up to 5:1 with high‐efficiency drive in the high‐speed, constant‐power region. The proposed IPMSM is suitable for variable‐speed drives requiring high‐speed, constant‐power operation. © 2000 Scripta Technica, Electr Eng Jpn, 134(3): 60–68, 2001