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91.
Rutao Luo E. Fabian Cardozo Michael J. Piovoso Hulin Wu Maria J. Buzon Javier Martinez-Picado Ryan Zurakowski 《Journal of the Royal Society Interface》2013,10(84)
A model of reservoir activation and viral replication is introduced accounting for the production of 2-LTR HIV-1 DNA circles following antiviral intensification with the HIV integrase inhibitor raltegravir, considering contributions of de novo infection events and exogenous sources of infected cells, including quiescent infected cell activation. The model shows that a monotonic increase in measured 2-LTR concentration post intensification is consistent with limited de novo infection primarily maintained by sources of infected cells unaffected by raltegravir, such as quiescent cell activation, while a transient increase in measured 2-LTR concentration is consistent with significant levels of efficient (R0 > 1) de novo infection. The model is validated against patient data from the INTEGRAL study and is shown to have a statistically significant fit relative to the null hypothesis of random measurement variation about a mean. We obtain estimates and confidence intervals for the model parameters, including 2-LTR half-life. Seven of the 13 patients with detectable 2-LTR concentrations from the INTEGRAL study have measured 2-LTR dynamics consistent with significant levels of efficient replication of the virus prior to treatment intensification. 相似文献
92.
Microbial polyhydroxyalkanoates (PHAs) have been a subject of significant research interest in the past few decades. The recent development of novel functionalized PHAs has opened up new possibilities to combine the good biocompatibility of PHA‐based drug delivery systems to, for example, improve drug loading and release properties, targeting or imaging functionalities. This mini‐review presents some recent scientific developments in the preparation of functionalized PHAs, PHA–drug and PHA–protein conjugates, multifunctional PHA nanoparticles and micelles as well as biosynthetic PHA particles for drug delivery. These developments in combination with the generally excellent biocompatibility of PHA materials are expected to further expand the interest in PHA materials for drug delivery and other therapeutic applications. © 2016 Society of Chemical Industry 相似文献
93.
A sequence S is nonrepetitive if no two adjacent blocks of S are the same. In 1906 Thue proved that there exist arbitrarily long nonrepetitive sequences over 3 symbols. We consider the online variant of this result in which a nonrepetitive sequence is constructed during a play between two players: Bob is choosing a position in a sequence and Alice is inserting a symbol on that position taken from a fixed set A. The goal of Bob is to force Alice to create a repetition, and if he succeeds, then the game stops. The goal of Alice is naturally to avoid that and thereby to construct a nonrepetitive sequence of any given length.We prove that Alice has a strategy to play arbitrarily long provided the size of the set A is at least 12. This is the online version of the theorem of Thue. The proof is based on nonrepetitive colorings of outerplanar graphs. On the other hand, one can prove that even over 4 symbols Alice has no chance to play for too long. The minimum size of the set of symbols needed for the online version of Thue?s theorem remains unknown. 相似文献
94.
Makrina A. Chairopoulou Alex Schreiber Fabian Kratzer Sebastian Wolf Ulrich Teipel 《化学,工程师,技术》2020,92(4):405-413
As a waste product, oyster shells pose a major environmental pollution problem and the reuse of the material is becoming increasingly important. Since the comminution process in the recycling of secondary raw materials defines the properties of the material, oyster shells were grinded using a planetary ball mill and an impact mill and the fractions obtained were analyzed in terms of size and shape and compared with each other. A comminuted material from the impact mill was used for a series of wetting experiments, which helped to demonstrate the surface free energy of the material. 相似文献
95.
Conventional fuels used for supplying internal combustion piston engines include petrols and diesel oils produced from petroleum. These are a non-renewable energy source. The environmental policy of the European Union is geared towards increasing the share of renewable fuels in the overall energy consumption. An alternative fuel originating from a renewable source, which could be used for feeding self-ignition internal combustion engines are the fatty acid methyl esters (FAME) of plant oils. The paper reports selected results of testing a 1.3 MULTIJET SDE 90 PS self-ignition engine with the Common Rail reservoir feed system supplied with mixtures of diesel oil and rape oil fatty acid methyl esters (FAME). Tests were carried out on an engine test bed equipped with an eddy-current brake. The purpose of the tests was to determine the economic–energy and ecological indices of engine operation. The concentrations of exhaust gas gaseous components were measured using a MEXA-1600DEGR analyzer, while the particulate concentrations, with a MEXA-1230PM analyzer. In addition, the variations of working medium pressures in the engine chamber and of fuel pressure upstream the injector were recorded as a function of crankshaft rotation angle using the AVL IndiSmart 612 indication system for this purpose. The physicochemical properties of fuels used in the tests were determined using a fuel analyzer. The obtained testing results made it possible to determine and assess the operation indices of the engine fed with mixtures of diesel oil and rape oil fatty acid methyl esters (FAME) with slightly higher ester contents than the requirements of the currently applicable diesel oil standard. 相似文献
96.
Tomasz Piechowiak Piotr Antos Radosław Józefczyk Patryk Kosowski Karol Skrobacz 《臭氧:科学与工程》2019,41(4):376-385
In the studies conducted, the impact of the innovative ozonation procedure on the microbial state and antioxidant potential of highbush blueberry (Vaccinum corymbosum L.) stored under cold storage conditions was assessed. Microbiological analysis was carried out to determine the total number of mesophilic aerobic bacteria and the total number of fungi during the storage experiment. In addition, changes in the flavonoid, anthocyanins, and vitamin C content and the total antioxidant capacity were monitored during the storage. The degree of fruit infection with gray mold and anthracnose was determined. It was found that daily ozonation of fruits with a dose of 15 ppm for 30 min, every 12 h, for 28 days effectively reduced the development of aerobic mesophilic bacteria and fungi. On the last day of storage, symptoms of the infection by gray mold were observed in 27.5% of the control fruit, while the absence of symptoms was observed in case of the ozonated fruit. On the other hand, ozone was ineffective in case of inhibiting the infection by anthracnose. Nevertheless, the ozonation process allowed maintaining a high antioxidant potential of the fruit and substantially reduced losses of flavonoids, anthocyanins, and vitamin C. The utilized procedure has proved to be effective, providing the possibility of extensive use of ozone as a factor allowing sustaining a high commercial and consumption value of the fruit over extended time. 相似文献
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