A review of the developments of characteristics of PEI derivatives for gene delivery applications

Redox‐active stimuli have gained a great deal of interest as an indicating factor for designing bioresponsive matrices in gene delivery. Hence, a wide range of gene carriers has been designed incorporating the redox‐stimuli characteristics. The most important type of gene carriers is the class of redox responsive polymers. Among them, disulfide incorporated redox‐responsive polyethyleneimine (PEI) and its derivatives, as a result of their outstanding DNA entrapping characteristics and their intrinsic endosomolytic activity, have attracted considerable attention in recent studies. The review presents the main developments of the characteristics of PEI derivatives and their applications in gene delivery. It is found that despite the uniquely stated characteristics, the noncleavable structure of conventional PEI (high molecular weight PEI: 25k), which makes it a nondegradable material, as well as the frequent inclusion of positively charged amino groups, which reduces its blood circulation period, render conventional PEI a very toxic material for gene‐delivery applications. The extremely high cellular toxicity of conventional PEI has restricted its administration for real in‐vivo physiological media. Recent studies have shown that employing low molecular weight PEI cross‐linked by disulfide linkages (SS‐PEI) and assembling low molecular weight disulfide linkages PEI (LMW SS‐PEI) with bio‐detachable anionic groups were two successful approaches for increasing bioavailability of the PEI‐based gene carriers, while keeping outstanding cellular transfection. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 42096.     

Kinetic rate-limiting steps in dehydrogenation of Li-N-H and Li-Mg-N-H systems - Effects of elemental Si and Al

The study is focused on the (LiNH₂ + LiH) and the (2LiNH₂ + MgH₂) hydrogen storage systems. Efforts were made to achieve an in-depth understanding of the dehydrogenation mechanisms following the introduction of elemental Si and Al to both systems. A variety of analytical instruments were employed, such as Temperature-Programmed Desorption, Mass Spectrometry, X-ray diffraction (XRD) and InfraRed spectroscopy. Unlike the effect of elemental Al in the (LiNH₂ + LiH) system, which showed no promising improvement in the dehydrogenation, a significant kinetic improvement in the (LiNH₂ + LiH) system was achieved upon addition of elemental Si. Kinetic improvement by elemental Si was described as a result of the LiH destabilisation through the formation of a Li₂Si phase and an increase in H⁻ anion concentration. On the other hand, once elemental Al is added to the (2LiNH₂ + MgH₂) system, the overall dehydrogenation kinetics of the system is delayed through the formation of a LiAl Phase. The results suggest that dehydrogenation mechanism in both the (LiNH₂ + LiH) and the (2LiNH₂ + MgH₂) systems are identical. Dehydrogenation reaction starts with the electrostatic interaction of the oppositely charged hydrogen atoms in amide and hydride and proceeds by mass transfer of reactant species across the product layer at the later stage of the dehydrogenation. However, it was particularly identified that each system has a unique kinetic rate limiting step. Dehydrogenation kinetics seems to be controlled by the diffusion of the H⁻ anion in the (LiNH₂ + LiH) system but by the diffusion of the Li⁺ cation in the (2LiNH₂ + MgH₂) system.     

Orientational Coupling Locally Orchestrates a Cell Migration Pattern for Re‐Epithelialization

Re‐epithelialization by collective migration of epithelial cells over a heterogeneous environment to restore tissue integrity and functions is critical for development and regeneration. Here, it is reported that the spatial organization of adjacent adherent paths within sparsely distributed extracellular matrix (ECM) has a significant impact on the orientational coupling between cell polarization and collective cell migration. This coupling effect determines the migration pattern for human keratinocytes to regain their cohesion, which impacts the occupancy of epithelial bridge and the migration velocity in wound repair. Statistical studies suggest the converging organization of ECM, in which adjacent paths become closer to each other and finally converge to a junctional point, facilitating collective cell migration mostly within variable ECM organization, as the polarization of the advancing cell sheet is remodeled to align along the direction of cell migration. The findings may help to design implantable ECM to optimize efficient skin regeneration.     

A stacked neural network approach for yield prediction of propylene polymerization

Prediction of reaction yield as the most important characteristic process of a slurry polymerization industrial process of propylene has been carried out. Stacked neural network as an effective method for modeling of inherently complex and nonlinear systems–especially a system with a limited number of experimental data points–was chosen for yield prediction. Also, effect of operational parameters on propylene polymerization yield was modeled by the use of this method. The catalyst system was Mg(OEt)₂/DIBP/TiCl₄/PTES/AlEt₃, where Mg(OEt)₂, DIBP (diisobutyl phthalate), TiCl₄, PTES (phenyl triethoxy silane), and triethyl aluminum (AlEt₃) (TEAl) were employed as support, internal electron donor (ID), catalyst precursor, external electron donor (ED), and co‐catalyst, respectively. The experimental results confirmed the validity of the proposed model. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2010     

Towards a self-adaptive service-oriented methodology based on extended SOMA

We propose a self-adaptive process (SAP) that maintains the software architecture quality using the MAPE-K standard model. The proposed process can be plugged into various software development processe...     

Development of ultrasmall chitosan/succinyl β‐cyclodextrin nanoparticles as a sustained protein‐delivery system

In this article, we introduce a new method for preparing ultrasmall chitosan (CS)/succinyl β‐cyclodextrin (SCD) nanoparticles (NPs) intended for loading bovine serum albumin (BSA) as a model protein. The proposed method is based on the complex coacervation technique followed by ionotropic gelation with tripolyphosphate. SCD, an anionic derivative of cyclodextrin, was synthesized and used in CS‐based NPs to enhance the entrapment efficiency of BSA. The results show that with this approach, ultrasmall, compact, and neutralized NPs with a mean particle size near 30 nm were obtained. A high degree of protein entrapment in the NPs led to a significant improvement in the BSA release profile with a low initial burst release (ca. 3% w/v of the initially loaded BSA) and a sustained release over time. This enabled a suitable nanocarrier for long‐term protein delivery (30% release over 120 h). © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131, 39648.     

Comprehensive features with randomized decision forests for hand segmentation from color images in uncontrolled indoor scenarios

Multimedia Tools and Applications - Hand segmentation is an integral part of many computer vision applications, especially gesture recognition. Training a classifier to classify pixels into hand or...     

Slip flow and heat transfer in rectangular and circular microchannels using hybrid FE/FV method

Rarefied gas flows typically encountered in MEMS systems are numerically investigated in this study. Fluid flow and heat transfer in rectangular and circular microchannels within the slip flow regime are studied in detail by our recently developed implicit, incompressible, hybrid (finite element/finite volume) flow solver. The hybrid flow solver methodology is based on the pressure correction or projection method, which involves a fractional step approach to obtain an intermediate velocity field by solving the original momentum equations with the matrix‐free, implicit, cell‐centered finite volume method. The Poisson equation resulting from the fractional step approach is then solved by node based Galerkin finite element method for an auxiliary variable, which is closely related to pressure and is used to update the velocity field and pressure field. The hybrid flow solver has been extended for applications in MEMS by incorporating first order slip flow boundary conditions. Extended inlet boundary conditions are used for rectangular microchannels, whereas classical inlet boundary conditions are used for circular microchannels to emphasize on the entrance region singularity. In this study, rarefaction effects characterized by Knudsen number (Kn) in the range of 0 ⩽ Kn ⩽ 0.1 are numerically investigated for rectangular and circular microchannels with constant wall temperature. Extensive validations of our hybrid code are performed with available analytical solutions and experimental data for fully developed velocity profiles, friction factors, and Nusselt numbers. The influence of rarefaction on rectangular microchannels with aspect ratios between 0 and 1 is thoroughly investigated. Friction coefficients are found to be decreasing with increasing Knudsen number for both rectangular and circular microchannels. The reduction in the friction coefficients is more pronounced for rectangular microchannels with smaller aspect ratios. Effects of rarefaction and gas‐wall surface interaction parameter on heat transfer are analyzed for rectangular and circular microchannels. For most engineering applications, heat transfer is decreased with rarefaction. However, for fluids with very large Prandtl numbers, velocity slip dominates the temperature jump resulting in an increase in heat transfer with rarefaction. Depending on the gas‐wall surface interaction properties, extreme reductions in the Nusselt number can occur. Present results confirm the existence of a transition point below and above wherein heat transfer enhancement and reduction can occur. Copyright © 2011 John Wiley & Sons, Ltd.