Label-free detection of melittin binding to a membrane using electrochemical-localized surface plasmon resonance

Localized surface plasmon resonance (LSPR) and electrochemistry measurements connecting to core-shell structure nanoparticle are successfully exploited in a simultaneous detectable scheme. In this work, the surface plasmon band characterizations of this nanostructure type are initially examined by controlling the core size of the silica nanoparticle and shell thickness of the deposited gold. These results clearly show that when the shell thickness is increased, keeping the core size constant, the peak wavelength of the LSPR spectra is shifted to a shorter wavelength and the maximum of peak intensity is achieved at a particular shell thickness. On the basis of this structure, we present a membrane-based nanosensor for optically detecting the binding of peptide toxin melittin to hybrid bilayer membrane (HBM) and electrochemically assessing its membrane-disturbing properties as a function of concentrations. It will open up the way to detect functionally similar protein toxins and other membrane-targeting peptides with the intension of integrating this chip into a microfluid and expanding it into multiarray format.     

Introduction to the Special Issue on Causal Discovery

Many empirical sciences, including the social sciences and life sciences, aim to study causal relationships. Researchers in these fields need computational methods for analyzing observed data and identifying causal structures among a set of variables. Such computational methods enable researchers to draw conclusions on the basis of both their assumptions and the observed data. Moreover, these methods are useful for developing hypotheses on causal relations, designing future observational studies, and planning future experimental studies that can potentially provide stronger evidence of estimated causal relations.

The objective of this special issue is to present an up-to-date overview of causal discovery methods, which have witnessed rapid advancements in recent years. The chief editor and guest editors invited the following three survey papers on various hot topics related to causal discovery:

     

Effect of water vapor on tritium permeation behavior in the blanket system

It is found that most hydrogen supplied to the purge gas changed to water vapor due to the water formation reaction in the early stage of the blanket operation and that physical or chemical adsorbed water is released in the high concentration into the blanket purge gas when the blanket temperature becomes higher than several hundreds of degrees K if the pre-treatment is not applied to the solid breeder materials. Effect of coexistence of water vapor in the purge gas on permeation behavior of hydrogen through F82H ferritic steel in the breeding part and palladium–silver (Pd–Ag) in the recovery part is discussed because use of them is generally considered for recovery of bred tritium from the solid blanket. Almost no decrease in permeation rate of F82H is observed in this study when water vapor exists in the blanket purge gas. The permeability of hydrogen isotopes through the Pd–Ag pipe gradually decreases when water vapor exists in the blanket purge gas. Properties required in estimation of the hydrogen permeated to the purge gas are experimentally obtained in this study.     

Nondestructive Hygiene Monitoring on Pork Meat Surface Using Excitation–Emission Matrices with Two-Dimensional Savitzky–Golay Second-Order Differentiation

To develop a rapid and nondestructive hygiene-monitoring system in meat-processing plants, plate count and ATP content on pork meat surfaces were quantitatively determined with particular attention to NAD(P)H fluorescence produced by microorganisms. An excitation (Ex)–emission (Em) matrix (EEM) was obtained, and the five fluorescence peaks of tryptophan, NAD(P)H, zinc protoporphyrin IX, protoporphyrin IX, and riboflavin were observed. Plate count and ATP content were predicted with good accuracy [r _p?=?0.90–0.94 and root mean square error of prediction (RMSEP)?=?log₁₀ (0.68–0.79 CFU cm^?2) for plate count and r _p?=?0.84–0.89 and RMSEP?=?log₁₀ (0.61–0.71, mol cm^?2) for ATP content]. Two-dimensional Savitzky–Golay second-order differentiation was found to be a powerful preprocessing tool of EEMs to improve prediction accuracy. Better prediction accuracy was obtained when the sensitivity of the fluorescence spectrophotometer was set to focus on fluorescence from NAD(P)H than that from both tryptophan and NAD(P)H. However, little linear relationship was observed between plate count and fluorescence intensity from NAD(P)H (R ²?=?0.31). The absolute value of regression coefficient (RC) of partial least-squares regression (PLSR) at the wavelength assigned to NAD(P)H, zinc protoporphyrin IX, protoporphyrin IX, and riboflavin was high. It can be concluded that a good prediction model was developed in which four fluorescence compounds of NAD(P)H, zinc protoporphyrin IX, protoporphyrin IX, and riboflavin contribute to the prediction model, and these compounds are probably produced by microorganisms.     

Immunomodulatory effects of polysaccharides produced by Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1

The extracellular polysaccharides (EPS) produced by lactic acid bacteria (LAB) are associated with the rheology, texture, and mouthfeel of fermented milk products, including yogurt. This study investigated the immunomodulatory effects of EPS purified from the culture supernatant of Lactobacillus delbrueckii ssp. bulgaricus (L. bulgaricus) OLL1073R-1. The crude EPS were prepared from the culture supernatant of L. bulgaricus OLL1073R-1 by standard chromatographic methods, and were fractionated into neutral EPS and acidic EPS (APS). Acidic EPS were further fractionated into high molecular weight APS (H-APS) and low molecular weight APS (L-APS). High molecular weight APS were shown to be phosphopolysaccharides containing D-glucose, D-galactose, and phosphorus. Stimulation of mouse splenocytes by H-APS significantly increased interferon-γ production, and, moreover, orally administered H-APS augmented natural killer cell activity. Oral administration of yogurt fermented with L. bulgaricus OLL1073R-1 and Streptococcus thermophilus OLS3059 to mice showed a similar level of immunomodulation as H-APS. However, these effects were not detected following administration of yogurt fermented with the starter combination of L. bulgaricus OLL1256 and S. thermophilus OLS3295. We conclude from these findings that yogurt fermented with L. bulgaricus OLL1073R-1, containing immunostimulative EPS, would have an immunomodulatory effect on the human body.     

Side-mode suppression mechanism of gain-coupled DFB lasers with periodically etched quantum wells

The effect of the gain and index coupling on the side-mode suppression ratio (SMSR) is studied for gain-coupled DFB lasers with periodically etched quantum wells. An accurate expression for the SMSR based on the amplified spontaneous emission model is used with the local-normal-mode transfer-matrix method. The mechanism for the strong single-mode stability of the gain-coupled DFB lasers is explained by the difference between the effective gain and loss of the Bloch waves in the grating structures. This new view clearly shows the advantage of the gain-coupled DFB lasers in terms of single-mode stability.     

Experimental study of LCI lasers fabricated by single MOCVDovergrowth followed by selective dopant diffusion

We report the design, fabrication, and characterization of lateral current injection (LCI) GaInAsP SQW lasers made using a single etch-and-regrowth followed by selective dopant diffusion. Devices are characterized electrically and optically from 10 to 300 K. Using the recently developed theory of the LCI laser, the threshold current, spontaneous efficiency, and stimulated efficiency are related to physical mechanisms that underlie the operation of this promising family of devices. We explore the prospects for LCI lasers to enable monolithic photonic integrated circuits and functional optoelectronic devices     

Relaxation oscillation frequency of DFB lasers with gain coupling

Using the spatially-dependent rate equations based on the Green's function analysis, we investigate the dependency of the relaxation oscillation frequency on the complex coupling coefficient and other parameters of gain-coupled DFB lasers by simultaneously considering spatial-hole-burning, gain saturation and gain compression. An explicit expression for the relaxation oscillation frequency for DFB lasers including the longitudinal spatial effects has been obtained. It is found that antiphase gain-coupling significantly enhances the local effective differential gain in the gain-coupled DFB laser and hence increases the relaxation oscillation frequency. We have also shown for the first time that the modal linewidth enhancement factor α_M plays an important role in determining the relaxation oscillation frequency of gain-coupled DFB lasers, especially when the built-in index coupling is weak