Trypsin from zebra blenny (Salaria basilisca) viscera: Purification,characterisation and potential application as a detergent additive

An alkaline trypsin was purified from the viscera of zebra blenny (Salaria basilisca) by ammonium sulphate (40?80% saturation) precipitation, Sephadex G-100, Mono Q-Sepharose and ultrafiltration. A yield of 12% with a purification-fold of 4.2 was obtained. The trypsin had an apparent molecular weight of 27 kDa. Soybean trypsin inhibitor and phenylmethylsulfonyl fluoride showed a strong inhibitory effect on the purified trypsin. Trypsin had maximal activity at pH 9.5 and 60 °C for the hydrolysis of Nα-benzoyl-dl-arginine-p-nitroanilide (BAPNA). It was stable at low temperatures and in the pH range of 7.0?12.0. The N-terminal amino acid sequence of the first 12 amino acids of the purified protease was IVGGRECTEPSQ. S. basilisca trypsin, which showed high homology with other fish trypsins, had a charged Arg residue at position 5, where Tyr is common in marine vertebrates and mammalian trypsins. The trypsin kinetic constants, K_m and k_cat for BAPNA, were 0.6 mM and 1.38 s^?1, respectively.     

Enhanced microwave absorbing properties of lightweight films based on polyaniline/aliphatic polyurethane composites in X band

Polymer blends based on nanostructured polyaniline (PANI) doped with hydrochloric acid (HCl) and para‐toluene sulfonic acid (PTSA) introduced into aliphatic polyurethane matrix (PU) are synthesized to produce flexible thin composite films for microwave absorbers. The effects of dopant type, PANI content and film thickness on morphologies, dielectric and microwave absorption properties in the X‐band are studied. It reveals that real and imaginary parts of the complex permittivity are proportional to filler concentrations and type of doped PANI. The PANI‐PTSA/PU films show higher permittivity and better microwave absorbing properties than PANI‐HCl/PU for the same weight fraction of PANI. The minimum reflection loss RL_(dB) values for the PANI‐PTSA/PU are ?37 dB at (20% PANI and 11.6 GHz) and ?30 dB at (15% PANI and 11.3 GHz) for thicknesses of 1.2 and 1.6 mm, respectively. These high values of reflection losses make the obtained lightweight and flexible composites promising radar absorbing materials (RAM). © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131, 40961.     

Methanol synthesis from CO2 and H2 in multi-tubular fixed-bed reactor and multi-tubular reactor filled with monoliths

This work investigates the impact of catalyst structuring into particles or monoliths on methanol production from only CO₂ and H₂ at a large scale. Methanol synthesis in multi-tubular reactors is evaluated using packed-bed and monolithic reactors by modeling heat and mass transfer in each reactor. The obtained simulation results show that, at low gas hourly space velocity (GHSV = 10,000 h⁻¹), the performances of both reactor technologies are similar. In this case, the packed-bed reactor technology is the most appropriate technology due to its simplicity of installation and operation. At high GHSV (25,000 h⁻¹), the packed-bed reactor technology is limited by a considerable pressure drop that causes an important loss in productivity due to thermodynamic equilibrium, whereas the monolithic reactors exhibit negligible pressure drop and achieve far better performances.     

Antioxidative Properties and Ability of Phenolic Compounds of Myrtus communis Leaves to Counteract In Vitro LDL and Phospholipid Aqueous Dispersion Oxidation

Antioxidant activities of Myrtus communis leaf phenolic compounds (McPCs) were investigated on 2,2′‐9‐azino‐bis‐3‐ethylbenzothiazoline‐6‐sulfonic acid (ABTS⁺?) and oxygen radical absorbance capacity (ORAC) tests or on oxidation of biological models, human low‐density lipoprotein (LDL) and phospholipid aqueous dispersion (l ‐α‐phosphatidylcholine stabilized by bile salts). Two extraction techniques, microwave‐assisted (MAE) and conventional (CE), were used to isolate McPCs, producing similar results of phenolic compound content. ABTS⁺? assay showed clearly that myrtle extracts exhibited a stronger scavenging effect than butylated hydroxyanisole and α‐tocopherol, with a slight advantage for myrtle CE extract. In ORAC assay, the both McPC extracts were similarly less effective than the pure compounds as caffeic acid and myricitrin (myricetin 3‐O‐rhamnoside) but stronger than butylated hydroxytoluene. Moreover, myrtle CE and MAE extracts, and myricitrin were able to inhibit similarly the production of conjugated dienes and to prolong the lag phase (Tlag) during Cu²⁺‐induced LDL oxidation with a dose‐response effect. The cryo‐electron microscopy observations on studied phospholipid dispersion stabilized by bile salts (BS) revealed the presence of bilayer vesicles and micelles. In 2,2′‐azobis (2‐amidinopropane) hydrochloride–induced phospholipid/BS oxidation, myrtle CE and MAE extracts gave similar effects to α‐tocopherol and caffeic acid but myricitrin showed a higher protective effect than myrtle extracts. We showed also that no synergic or additive effect between α‐tocopherol and myrtle extracts or caffeic acid in α‐tocopherol–enriched phospholipid/BS dispersion, but myricitrin showed an additive effect and thus promoted the total antioxidant activity. These data showed that myrtle extract could be used as potential natural antioxidants, food stabilizers, or natural health products.     

Experimental study of PLA thermal behavior during fused filament fabrication

Fused filament fabrication (FFF) is an additive manufacturing technique that is used to produce prototypes and a gradually more important processing route to obtain final products. Due to the layer-by-layer deposition mechanism involved, bonding between adjacent layers is controlled by the thermal energy of the material being printed, which strongly depends on the temperature development of the filaments during the deposition sequence. This study reports experimental measurements of filament temperature during deposition. These temperature profiles were compared to the predictions made by a previously developed model. The two sets of data showed good agreement, particularly concerning the occurrence of reheating peaks when new filaments are deposited onto previously deposited ones. The developed experimental technique is shown to demonstrate its sensitivity to changing operating conditions, namely platform temperature and deposition velocity. The data generated can be valuable to predict more accurately the bond quality achieved in FFF parts.     

Measurement of Workability of Fresh Concrete Using a Mixing Truck

The main objective of this study is to evaluate the workability of fresh portland cement concrete while it is still in the mixing truck by determining fundamental rheological parameters (plastic viscosity and yield stress). Nine concrete mixtures with different values of yield stress and plastic viscosity were tested in a concrete truck. The measurements made with the truck were based on the typical method of determining the flow behavior in a traditional fluid rheometer; that is, the shear rate in the mixing truck was swept from high to low by varying the rotation speed of the drum. The results of these experiments are discussed and compared with data provided by the ICAR rheometer, a portable rheometer designed for measuring concrete rheology. The test results indicate that the mixing truck equipment is sufficiently sensitive to detect differences in yield stress, slump, and plastic viscosity. However, the plastic viscosity determined by the truck measurement did not correlate with plastic viscosity as measured by the ICAR rheometer, while the yield stress determined by the truck measurement did correlate well with the measured slump and the ICAR rheometer resultsSuggestions are given on how to improve the mixing truck for better use as a rheometer.     

A distance measure for large graphs based on prime graphs

Graphs are universal modeling tools. They are used to represent objects and their relationships in almost all domains: they are used to represent DNA, images, videos, social networks, XML documents, etc. When objects are represented by graphs, the problem of their comparison is a problem of comparing graphs. Comparing objects is a key task in our daily life. It is the core of a search engine, the backbone of a mining tool, etc. Nowadays, comparing objects faces the challenge of the large amount of data that this task must deal with. Moreover, when graphs are used to model these objects, it is known that graph comparison is very complex and computationally hard especially for large graphs. So, research on simplifying graph comparison gainedan interest and several solutions are proposed. In this paper, we explore and evaluate a new solution for the comparison of large graphs. Our approach relies on a compact encoding of graphs called prime graphs. Prime graphs are smaller and simpler than the original ones but they retain the structure and properties of the encoded graphs. We propose to approximate the similarity between two graphs by comparing the corresponding prime graphs. Simulations results show that this approach is effective for large graphs.     

Investigation of new micromachined coplanar probe for near-field microwave microscopy

Microsystem Technologies - The near-field measurement is based generally on the use of purely passive miniature sensors, dedicated to the capture of a component of the electromagnetic field. The...     

A Higher-Order Chimera Method for Finite Volume Schemes

In this work a higher-order accurate finite volume method for the resolution of the Euler/Navier–Stokes equations using Chimera grid techniques is presented. The formulation is based on the use of Moving Least Squares approximations in order to obtain higher-order accurate reconstruction and connectivity between the overlapped grids. The accuracy and performance of the proposed methodology is demonstrated by solving different benchmark problems.