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951.
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953.
Our aim was to carefully analyse the time-dependent changes that affect the mitochondrial function of myocardial cells during and after an ischemic episode. To this end, variables characterizing mitochondrial function have been evaluated on myocardial samples from isolated rat hearts subjected to different conditions of ischemia. The technique of permeabilized fibers was used in order to evaluate the mitochondrial function whilst retaining intracellular structure. The earliest alteration that could be detected was a decrease in the stimulatory effect of creatine on mitochondrial respiration. This alteration became more pronounced as the severity (or duration) of the ischemia increased. Afterwards, a significant decrease in the apparent Km of mitochondrial respiration for ADP also appeared, followed by a diminution of the maximal respiration rate which was partly restored by adding cytochrome c. Finally, for the most severe conditions of ischemia, the basal respiratory rate also increased. These observations are indicative of a sequence of alterations affecting first the intermembrane space, then the outer mitochondrial membrane, and finally the inner membrane. The discussion is focused on the very early alterations, that could not be detected using the conventional techniques of isolated mitochondria. We postulate that these alterations to the intermembrane space and outer mitochondrial membrane can induce disturbances both in the channelling of energy from the mitochondria, and on the signalling towards the mitochondria. The potential consequences on the regulation of the production of energy (ATP, PC) by the mitochondria are evoked. 相似文献
954.
DM Watts V Lavera J Callahan C Rossi MS Oberste JT Roehrig CB Cropp N Karabatsos JF Smith DJ Gubler MT Wooster WM Nelson CG Hayes 《Canadian Metallurgical Quarterly》1997,56(6):661-667
An outbreak of a febrile illness characterized by headache, ocular pain, myalgia, and arthralgia occurred during June 1994 among Peruvian army troops in Northern Peru. On June 14-16, 1994, clinical data and blood samples were obtained from eight soldiers with a febrile illness, and from 26 others who had a history of febrile illness during the past three months. A follow-up blood sample was obtained 107 days later from four of the febrile and seven of the afebrile soldiers. Serum samples were tested for dengue (DEN), Oropouche (ORO), and Venezuelan equine encephalitis (VEE) IgM and IgG antibodies by an enzyme-linked immunosorbent assay (ELISA). Virus isolation was performed by inoculation of newborn mice and Vero cell cultures. Viral isolates were identified by immunofluorescence, ELISA, and nucleotide sequencing. A VEE virus infection was confirmed in three of the eight febrile soldiers, two by virus isolation, and one by serology. Antigenic analysis indicated that one of the virus isolates was similar to VEE subtype I, variety ID, viruses previously isolated in Colombia and Venezuela. Nucleotide sequence data showed that both viral isolates were identical to one another and closely related to VEE ID viruses previously isolated in Peru, Colombia, and Venezuela. Serologic results showed that two of 26 afebrile soldiers had IgM antibody to VEE and four had IgG antibody to VEE; two febrile soldiers had IgG antibody in their first serum samples. Oropouche-specific IgM antibody was detected in one of the eight febrile and five of the afebrile soldiers, and 18 of the 34 soldiers had low titers of ORO IgG antibody titers, which did not meet the diagnostic criteria for confirmed cases. All soldiers were negative for DEN IgM antibody, and 10 had flavivirus IgG antibody that reacted with DEN antigens. These data indicated that VEE ID virus was one of the causes of illness among Peruvians soldiers and that this was the first association of this VEE subtype with human disease in Peru. 相似文献
955.
PR Castello FL González Flecha AJ Caride HN Fernández JM Delfino JP Rossi 《Canadian Metallurgical Quarterly》1997,6(8):1708-1717
A systematic study of the membrane-associated regions in the plasma membrane Ca2+ pump of erythrocytes has been performed by hydrophobic photolabeling. Purified Ca2+ pump was labeled with 3-(trifluoromethyl)-3-(m-[125I]iodophenyl)-diazirine ([125I]TID), a generic photoactivatable hydrophobic probe. These results were compared with the enzyme labeled with a strictly membrane-bound probe, [3H]bis-phosphatidylethanolamine (trifluoromethyl) phenyldiazirine. A significant light-dependent labeling of an M(r) 135,000-140,000 peptide, corresponding to the full Ca2+ pump, was observed with both probes. After proteolysis of the pump labeled with each probe and isolation of fragments by SDS-PAGE, a common pattern of labeled peptides was observed. Similarly, labeling of the Ca2+ pump with [125I]TID, either in isolated red blood cell membranes or after the enzyme was purified, yields a similar pattern of labeled peptides. Taken together, these results validate the use of either probe to study the lipid interface of the membrane-embedded region of this protein, and sustain the notion that the conformation of the pump is maintained throughout the procedures of solubilization, affinity purification, and reconstitution into proteoliposomes. In this work, we put special emphasis on a detailed analysis of the N-terminal domain of the Ca2+ pump. A labeled peptide of M(r) 40,000 belonging to this region was purified and further digested with V8 protease. The specific incorporation of [125I]TID to proteolytic fragments pertaining to the amino-terminal region indicates the existence of two transmembrane stretches in this domain. A theoretical analysis based on the amino acid sequence 1-322 predicts two segments with high probability of membrane insertion, in agreement with the experimental data. Each segment shows a periodicity pattern of hydrophobicity and variability compatible with alpha-helical structure. These results strongly suggest the existence of a transmembrane helical hairpin motif near the N-terminus of the Ca2+ pump. 相似文献
956.
The paper points out that a certain iterative method of direct deconvolution is a particular manifestation of the so-called Expectation-Maximisation (EM) algorithm. Recognition of this leads to the establishment of general convergence results by reference to existing work. 相似文献
957.
The galvanic pyrophosphate copper bath is long-lasting, reliable, easy to run and produces a good quality deposit even in continuous operation. It is therefore widely used in industry both for the protection of ferrous materials and in the manufacture of brass-plated cords for radial tyres. However, this type of bath is particularly sensitive to impurities. This paper presents the results of a voltammetric study on the effects of chloride, sulphate, nitrate and phosphate anions and ammonia on the anodic process in an industrial pyrophosphate copper bath. 相似文献
958.
可编程逻辑器件为数字设计中复杂功能的实现提供了一种流行的方法。虽然制造商尚未提供能与VLSI数字电路复杂性相比拟的模拟电路,但现场可编程模拟电路正在信号调整和滤波应用中获得广泛采用。这些器件基于CMOS跨导及开关式电容放大器,可为相对复杂的设计问题提供一种便利的解决方案。Lattice 相似文献
959.
The main lattice of the Large Hadron Collider (LHC) employs about 1600 main magnets and more than 4000 corrector magnets. All superconducting and working in pressurized superfluid helium bath, these impressive line of magnets fills more than 20 km of the underground tunnel. With almost 70 main dipoles already delivered and 10 main quadrupoles almost completed, we passed the 5% of the production and now all manufacturers have fully entered into series production. In this paper the most critical issues encountered in the ramping up in such a real large scale fabrication is addressed; uniformity of the coil size and of prestress, special welding technique, tolerances on curvature (dipoles) or straightness (quadrupoles) and of the cold mass extremities, harmonic content and, most important, the integrated field uniformity among magnets. The actual limits and the solution for improvements are discussed. Finally a realistic schedule based on actual achievements is presented. 相似文献
960.
Pierre Rossi 《Materials and Structures》1994,27(6):319-323
The dynamic behaviour of concrete involves both material and structural aspects. Concerning the material level, the Laboratoire Central des Ponts et Chaussées has cooperated with Delft University of Technology within the framework of a European project. Two problems were studied: the influence of free water in the concrete pores on the rate effects (dynamic strength and static strength) observed in the mechanical behaviour of the material, and the influence of the water/cement ratio on these rate effects. It was found that the rate effects are due to the influence of free water in the hydrate pores. Also, the rate effects depend on the water/cement ratio. They decrease with increasing ratio, but are independent of the absolute value (dynamic strength—static strength). Concerning the structural level, the LCPC has cooperated with LEA (Shelter Testing Laboratory) to carry out a study on the shock behaviour of concrete slabs using a shock tube. At a structural level, the rate effects on the mechanical behaviour of the slabs seem to be due to the presence of water in the material. It was further found that there is a change of failure mechanism in the slabs, i.e., a transition from a bending failure mechanism to a shear failure mechanism, with increasing loading rate and with decreasing water/cement ratio. 相似文献