Biosynthetic Plasticity Enables Production of Fluorinated Aurachins

Enzyme promiscuity has important implications in the field of biocatalysis. In some cases, structural analogues of simple metabolic building blocks can be processed through entire pathways to give natural product derivatives that are not readily accessible by chemical means. In this study, we explored the plasticity of the aurachin biosynthesis pathway with regard to using fluoro- and chloroanthranilic acids, which are not abundant in the bacterial producers of these quinolone antibiotics. The incorporation rates of the tested precursor molecules disclosed a regiopreference for halogen substitution as well as steric limitations of enzymatic substrate tolerance. Three previously undescribed fluorinated aurachin derivatives were produced in preparative amounts by fermentation and structurally characterized. Furthermore, their antibacterial activities were evaluated in comparison to their natural congener aurachin D.     

Continuous generating grinding—Tooth root machining and use of CBN-tools

Profile accuracy, no burning and residual compressive stresses at the tooth root fillet are required for the durability of highly stressed gears. This paper reveals the challenges for continuous generating grinding with corundum and CBN. For this purpose, material removal simulations and experimental investigations were carried out to gain knowledge of the tool–workpiece contact conditions. The potential of CBN tools was analysed due to the fact that the mechanical loads at the grinding worm tip result in high profile wear of the corundum tools. In this context, especially the interrelationship between the dressing strategy and the workpiece quality was investigated in detail.     

Topology optimization for crashworthiness of thin-walled structures under axial impact using hybrid cellular automata

Although topology optimization is well established in most engineering fields, it is still in its infancy concerning highly non-linear structural applications like vehicular crashworthiness. One of the approaches recently proposed and based on Hybrid Cellular Automata is modified here such that it can be applied for the first time to thin-walled structures. Classical methods based on voxel techniques, i.e., on solid three-dimensional volume elements, cannot derive structures made from thin metal sheets where the main energy absorption mode is related to plastic buckling, folding and failure. Because the main components of car structures are made from such thin-walled beams and panels, a special approach using SFE CONCEPT was developed, which is presented in this paper.     

Distribution and identification of culturable airborne microorganisms in a Swiss milk processing facility

Airborne communities (mainly bacteria) were sampled and characterized (concentration levels and diversity) at 1 outdoor and 6 indoor sites within a Swiss dairy production facility. Air samples were collected on 2 sampling dates in different seasons, one in February and one in July 2012 using impaction bioaerosol samplers. After cultivation, isolates were identified by mass spectrometry (matrix-assisted laser desorption/ionization-time-of-flight) and molecular (sequencing of 16S rRNA and rpoB genes) methods. In general, total airborne particle loads and total bacterial counts were higher in winter than in summer, but remained constant within each indoor sampling site at both sampling times (February and July). Bacterial numbers were generally very low (<100 cfu/m³ of air) during the different steps of milk powder production. Elevated bacterial concentrations (with mean values of 391 ± 142 and 179 ± 33 cfu/m³ of air during winter and summer sampling, respectively; n = 15) occurred mainly in the “logistics area,” where products in closed tins are packed in secondary packaging material and prepared for shipping. However, total bacterial counts at the outdoor site varied, with a 5- to 6-fold higher concentration observed in winter compared with summer. Twenty-five gram-positive and gram-negative genera were identified as part of the airborne microflora, with Bacillus and Staphylococcus being the most frequent genera identified. Overall, the culturable microflora community showed a composition typical and representative for the specific location. Bacterial counts were highly correlated with total airborne particles in the size range 1 to 5 µm, indicating that a simple surveillance system based upon counting of airborne particles could be implemented. The data generated in this study could be used to evaluate the effectiveness of the dairy plant’s sanitation program and to identify potential sources of airborne contamination, resulting in increased food safety.     

Dynamic mechanical behavior and nanostructure morphology of hyperbranched‐modified polypropylene blends

Polypropylene (PP) was modified utilizing two types of polyesteramide‐based hyperbranched polymers (amphiphilic PS and hydrophilic PH). A maleicanhydride‐modified PP (PM) was used as a reactive dispersing agent to enhance the modification by grafting the hyperbranched polymers onto the PP chains. Pure PP, two different non‐reactively modified samples, i.e. excluding PM, and two different reactively modified samples, i.e. including PM, were studied. Investigating the morphology of the samples was performed by scanning electron microscopy. To follow the effect of the modification on the dynamic mechanical properties, dynamic mechanical analysis experiments both in the melt (rheometric mechanical spectrometry) and in solid state (dynamic mechanical thermal analysis) were carried out. In the next step, the nanocrystalline structure of the samples was studied by small angle X‐ray scattering (SAXS) in two different modes, i.e. static and recrystallization. Hundreds of SAXS patterns were analyzed automatically using procedures written in PV‐WAVE image‐processing software. The chord distribution function (CDF) was calculated and the long period (l_p) of the crystal lamellae was extracted from the CDFs. The rheometric mechanical spectrometry results show that both hyperbranched polymers decrease complex viscosity η^* and enhance liquid‐like behavior. This happens more significantly when PM is included. The dynamic mechanical thermal analysis results reveal that T_g decreases when PS and PH are added. In the reactively modified samples this reduction is compensated most probably because of the crosslinked structure formed through the grafting reaction between the hyperbranched polymers and PM. Such structure is confirmed by SAXS data and calculated CDFs in the recrystallization mode. Static SAXS data also show enhancement in the crosshatched morphology of the crystalline lamellae of PP for reactively modified samples compared with non‐reactively modified samples. © 2013 Society of Chemical Industry     

p‐TCOs für Transparente Elektronik

p‐type TCOs for use in transparent electronics In this article, a new type of semiconducting material is introduced. Transparent conducting thin films are already established in our everydaylife. But the type of these so‐called ?TCOs”? is dominated by electron transport, that means they are n‐type. To get transparent p‐n‐junctions and therefore transparent electronics, the missing part is a transparent p‐type material. Researchers all over the world have found different approach to produce this new generation of materials. At the Fraunhofer Institute for Surface Engineering and Thin Films (IST) in Braunschweig it is now possible to produce these kind of thin films as well.     

Zwitterionic‐Coated “Stealth” Nanoparticles for Biomedical Applications: Recent Advances in Countering Biomolecular Corona Formation and Uptake by the Mononuclear Phagocyte System

Nanoparticles represent highly promising platforms for the development of imaging and therapeutic agents, including those that can either be detected via more than one imaging technique (multi‐modal imaging agents) or used for both diagnosis and therapy (theranostics). A major obstacle to their medical application and translation to the clinic, however, is the fact that many accumulate in the liver and spleen as a result of opsonization and scavenging by the mononuclear phagocyte system. This focused review summarizes recent efforts to develop zwitterionic‐coatings to counter this issue and render nanoparticles more biocompatible. Such coatings have been found to greatly reduce the rate and/or extent of non‐specific adsorption of proteins and lipids to the nanoparticle surface, thereby inhibiting production of the “biomolecular corona” that is proposed to be a universal feature of nanoparticles within a biological environment. Additionally, in vivo studies have demonstrated that larger‐sized nanoparticles with a zwitterionic coating have extended circulatory lifetimes, while those with hydrodynamic diameters of ≤5 nm exhibit small‐molecule‐like pharmacokinetics, remaining sufficiently small to pass through the fenestrae and slit pores during glomerular filtration within the kidneys, and enabling efficient excretion via the urine. The larger particles represent ideal candidates for use as blood pool imaging agents, whilst the small ones provide a highly promising platform for the future development of theranostics with reduced side effect profiles and superior dose delivery and image contrast capabilities.     

Characterization of a miniaturized liquid bridge for nL sample infusion: a comparative study of sample flush-out behavior using flow simulations and direct ESI-MS analysis

In this work we shed light on the microfluidics of a miniaturized liquid bridge that forms the central part of a so-called “capillary gap sampler,” a novel device for rapid and seamless injection of nanoliter sample volumes into an electrospray ionization mass spectrometer (ESI-MS). Parameters relevant for sample flush-out at the liquid bridge and in the spray capillary were identified by systematic variation of the capillary dimensions, the linear buffer flow rate (2.1–34 mm/s) and molecular weight of the analytes (0.5–30 kDa). We found that a reduction in capillary wall thickness by a factor of 1.6 significantly influences analyte peak shapes, leads to an inversion of the relationship between peak width and analyte molecular weight, and allows a fivefold decrease in peak width for large molecules down to 5 s. The results could be verified and explained by simulations, in which the presence of diffusion-controlled “dead zones” at the liquid bridge and dispersion in the spray tip that depend on analyte molecular weight were identified as key factors relevant for the sample flush-out process. The merging of simulations and experimental data gives useful hints toward the re-design of a spray tip as built-in ESI-MS interface for an optimized gap sampler performance.