An optimal model for representing the kinetics of growth and product formation by Lactobacillus rhamnosus on multiple substrates

A comprehensive model was developed to simulate Lactobacillus rhamnosus growth on a medium containing multiple limiting carbon sources. The strategy of optimizing specific growth rate to predict growth on multiple substrates was demonstrated. The model predictions were based on parameters obtained from L. rhamnosus growth on individual substrates. The model was able to simulate the growth, substrate consumption, product formation and specific growth rate profiles of L. rhamnosus accurately. The model prediction that co-metabolism of glucose and pyruvate enhances growth rate of and flavor production by the bacterium was experimentally verified.     

Formation of neutral molecules of potential stellar interest by neutralisation of negative ions in a mass spectrometer. The application of experiment and molecular modelling in concert

This paper is a modified version of a lecture which describes the synthesis, structure and reactivity of some neutral molecules of stellar significance. The neutrals are formed in the collision cell of a mass spectrometer following vertical Franck-Condon one electron oxidation of anions of known bond connectivity. Neutrals are characterised by conversion to positive ions and by extensive theoretical studies at the CCSD(T)/aug-cc-pVDZ//B3LYP/6-31G(d) level of theory. Four systems are considered in detail, viz (i) the formation of linear C(4) and its conversion to the rhombus C(4), (ii) linear C(5) and the atom scrambling of this system when energised, (iii) the stable cumulene oxide CCCCCO, and (iv) the elusive species O(2)C-CO. This paper is not intended to be a review of interstellar chemistry: examples are selected from our own work in this area.     

Pullout Behavior of Granular Pile-Anchors in Expansive Clay Beds In Situ

Granular pile anchors (GPA) are one of the recent innovative foundation techniques devised for mitigating the problems posed by swelling clay beds. In a granular pile anchor, the footing is anchored to an anchor plate at the bottom of the granular pile. This makes the granular pile tension resistant and enables it to absorb the tensile force caused on the foundation by the swelling clay. An understanding of the amount of uplift resistance offered by the GPA is important in the design of granular pile-anchor foundations in field situations causing tensile forces on foundations, such as in expansive clay beds. This paper presents the results of a field-scale test program conducted to study the pullout response of GPAs embedded in expansive clay beds. Pullout load tests were conducted on GPAs of varying lengths and diameters. It was found from the field pullout load tests that granular pile anchors of larger surface area resulted in higher pullout capacity. Of the various single granular pile anchors with l/d values between 2.5 and 10, the GPA of length 1000?mm and diameter 200?mm (l/d = 5) showed the best pullout load response when tested alone, resulting in a failure uplift capacity of 14.71?kN. Increase in diameter and length of granular pile anchor increased the uplift capacity. When the length of the GPA was increased from 500 to 750 and 1000?mm, the percentage increase in the uplift load required for an upward movement of 25?mm was 33.3 and 55.5% respectively. The pullout load of the GPA when tested under group was 18?kN as against a 12?kN for the GPA when tested single.     

Exosomes in bodily fluids are a highly stable resource of disease biomarkers

Biomarkers are measurable indicators of a biological state. As our understanding of diseases meliorates, it is generally accepted that early diagnosis renders the best chance to cure a disease. In the context of proteomics, the discovery phase of identifying bonafide biomarkers and the ensuing validation phase involving large cohort of patient samples are impeded by the complexity of bodily fluid samples. High abundant proteins found in blood plasma make it difficult for the detection of low abundant proteins that may be potential biomarkers. Extracellular vesicles (EVs) have reignited interest in the field of biomarker discovery. EVs contain a tissue-type signature wherein a rich cargo of proteins and RNA are selectively packaged. In addition, as EVs are membranous structures, the luminal contents are protected from degradation by extracellular proteases and are highly stable in storage conditions. Interestingly, an appealing feature of EV-based biomarker analysis is the significant reduction in the sample complexity compared to whole bodily fluids. With these prescribed attributes, which are the rate-limiting factors of traditional biomarker analysis, there is immense potential for the use of EVs for biomarker detection in clinical settings. This review will discuss the current issues with biomarker analysis and the potential use of EVs as reservoirs of disease biomarkers.     

Development of HDPE/EPDM‐g‐TMEVS nanocomposites with enhanced electrical and flame properties

Nanoclay reinforced HDPE/silane grafted EPDM composites have been developed using an epoxy functionalized HDPE as compatibilizer.The nanoclay has been varied from 0% to 10% in the composites along with the incorporation of compatibilizer and without compatibilizer in a brabender plasticorder.The dielectric and fire retardant properties of these nanocomposites have been examined. Addition of nanoclay enhanced char formation with increased values of limiting oxygen index. Electrical properties such as volume and surface Resistivity improved with addition of nanoclay and compatibilizer. The values of tan δ increased with increase in grafted EPDM and silanated nanoclay loading. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013     

Synthesis of cobalt-doped barium cerate-zirconate and its evaluation for hydrogen production and electrochemical characterization

Cobalt-doped barium cerate-zirconate was synthesized using an oxalate co-precipitation route. The material was characterized using X-ray diffraction, transmission and scanning transmission electron microscopy coupled with X-ray energy dispersive spectroscopy. Results indicated that homogeneous cubic phase material was obtained at very high heat-treatment temperatures. Catalytic activity of the material toward CH₃OH partial-oxidation was tested at different temperatures and O₂:CH₃OH ratios. High hydrogen yields were obtained indicating that the material was a suitable catalyst for hydrogen generation. Impedance spectroscopy tests were conducted at different conditions to understand conduction processes occurring in the material. Results suggested mixed protonic–electronic conductivity in the presence of hydrogen. Thus, material is a potential candidate for the bi-functional role of electro-ceramic catalyst for simultaneous hydrogen generation and purification.     

Creating Shared Information Spaces to Support Collaborative Design Work

The provision of computer support for collaborative work is a central concern for Information Systems (IS) research and practice. In this paper we present the details of an information flow study undertaken in the household division of a large European design and manufacturing company (Delta). The goal of the study was to elicit user requirements for a computer system to facilitate the collaborative work of new product design teams. These requirements form the basis for the design and development of a Web-based LIRÉ (Living Repository) prototype system, the functionalities, features, and rationale of which are discussed. We also present the results of the evaluation of LIRÉ by the users at Delta and our observations on enhancing the sophistication and usefulness of this class of system. A production version based on the prototype but using commercially available software is currently in use by the New Product Development group at Delta.