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571.
We have developed a lipase-facilitated supported liquid membrane. Lipase-catalyzed reactions were coupled with a supported liquid membrane (SLM) to transport organic acids through the SLM. We succeeded in the rational transport of organic acids through the SLM using lipase-catalyzed reactions and observed that there were differences in the transport behavior of various organic acids due to the substrate specificity of lipase. Subsequently, various parameters, such as the alcohol concentration in the feed phase, the pH in each aqueous phase, an organic solvent in the SLM, and the kind of lipase, were investigated. We found that the optimum conditions were 65 vol% alcohol concentration, pH 6.3 in each aqueous phase, isooctane as the liquid membrane phase and Candida rugosa lipase as the esterification biocatalyst. 相似文献
572.
Tao?ChenEmail author Meng-Yue?Wu Ryoichi?Ishihara Kenji?Nomura Toshio?Kamiya Hideo?Hosono C.?I.?M Beenakker 《Journal of Materials Science: Materials in Electronics》2011,22(11):1694-1696
In this paper, we were able to crystallize InGaZnO4 (IGZO) by excimer laser on SiO2 substrate. It was observed that uniform [0001] textured polycrystalline IGZO film has been obtained without any grain boundaries
and oxygen vacancies on SiO2 substrate. This process is very promising in fabricating high quality IGZO thin film transistors (TFT) at low temperature
without seed substrate. 相似文献
573.
Hoi Lok Cheng Michael T. Tang Wasin Tuchinda Kazuyuki Enomoto Atsuya Chiba Yuichi Saito Tomihiro Kamiya Masaki Sugimoto Akinori Saeki Tsuneaki Sakurai Masaaki Omichi Daisuke Sakamaki Shu Seki 《Advanced Materials Interfaces》2015,2(1)
The preparation of photoresponsive polymer nanowires comprising photochromic azobenzene (Azo) and π‐conjugated fluorene (FO) units is reported. Well‐defined and uniform nanowires of the copolymer (PFOAzo) were successfully fabricated by the single particle nanofabrication technique after optimizing the FO:Azo ratio and the development conditions. Azo units in the PFOAzo nanowires underwent reversible trans–cis–trans isomerization upon exposure to ultraviolet or visible light, leading to changes in the radius (between ca. 6 and 8 nm) and morphology (straight or wavy) of the nanowires. The oligo(alkylfluorene) units in the backbone are found to profit the crosslinking efficiency upon high‐energy ion beam irradiation, and more importantly, provide sufficient flexibility to allow reversible photoswitching. This demonstration of the photoluminescence, semiconducting, and mechanical properties of the PFOAzo nanowires is an important advance in the evolution of electro‐mechanical nanomaterials. 相似文献
574.
Ultrasensitive Molecular Beacon Designed with Totally Serinol Nucleic Acid (SNA) for Monitoring mRNA in Cells 下载免费PDF全文
Keiji Murayama Dr. Yukiko Kamiya Dr. Hiromu Kashida Prof. Dr. Hiroyuki Asanuma 《Chembiochem : a European journal of chemical biology》2015,16(9):1298-1301
An artificial nucleic acid based on acyclic serinol building blocks and termed “serinol nucleic acid” (SNA) was used to construct a fluorescent probe for RNA visualization in cells. The molecular beacon (MB) composed of only SNA with a fluorophore at one terminus and a quencher at the other was resistant to enzymatic digestion, due to its unnatural acyclic scaffold. The SNA‐MB could detect its complementary RNA with extremely high sensitivity; the signal‐to‐background (S/B) ratio was as high as 930 when perylene and anthraquinone were used as the fluorophore and quencher pair. A high S/B ratio was also achieved with SNA‐MB tethering the conventional Cy3 fluorophore, and this probe enabled selective visualization of target mRNA in fixed cells. Thus, SNA‐MB has potential for use as a biological tool capable of visualizing RNA in living cells. 相似文献
575.
Kamiya N Doi S Tanaka Y Ichinose H Goto M 《Journal of Bioscience and Bioengineering》2007,104(3):195-199
Covalent and site-specific protein immobilization catalyzed by microbial transglutaminase (MTG) was investigated using recombinant Escherichia coli alkaline phosphatase (AP) tagged with a glutamyl donor substrate peptide (MLAQGS) of MTG. A polystyrene surface physically coated with beta-casein or bovine serum albumin (BSA) was employed as an MTG-specific surface displaying reactive lysine residues. MTG-mediated protein immobilization through catalytic varepsilon-(gamma-glutamyl)lysine bond formation between the peptide tag of recombinant APs and beta-casein- or BSA-coated surface was verified by the detection of AP activity on the surface. It was found that the length and the insertion position of the peptide tag did not significantly affect the efficacy of enzymatic immobilization of the recombinant APs. On the other hand, pH and ionic strength in the reaction media had crucial effects on the immobilization yields. Interestingly, the optimum pH range of MTG-mediated protein immobilization differed markedly from that for an MTG-catalyzed reaction in aqueous solution. The results suggest that the concentration of reactive species due to electrostatic interaction between the enzyme-substrate intermediate and the protein-adsorbed surface is a key factor governing MTG catalysis at a solid surface. 相似文献
576.
Kohei Kamiya Wakako Hamabe Shogo Tokuyama Ken Hirano Toshiko Satake Yuko Kumamoto-Yonezawa Hiromi Yoshida Yoshiyuki Mizushina 《Food chemistry》2010
During the course of our studies to develop new uses for the Noni (Morinda citrifolia) root, 10 anthraquinones, rubiadin (1), rubiadin 1-methyl ether (2), lucidin (3), damnacanthol (4), 1,3-dihydroxy-2-methoxymethylanthraquinone (5), 3-hydroxy-1-methoxy-2-methoxymethylanthraquinone (6), nordamnacanthal (7), damnacanthal (8), sorandidiol (9) and morindone (10), were isolated. Compounds 5, 6, 7, 8 and 10 exhibited remarkable inhibition against the activities of animal pols, and compound 10 was the strongest inhibitor in the anthraquinones investigated. Among mammalian pols, compound 10 inhibited the pol activities of A- (pol γ), B- (pols α, δ and ε) and Y- (pols η, ι and κ) families, but did not influence the activities of X-family pols (pols β, λ and terminal deoxynucleotidyl transferase). The tendency of pol inhibition showed a positive correlation with the suppression of human colon cancer cell HCT116 growth. These results suggested that the Noni root containing anthraquinones may be used as an anticancer functional food. 相似文献
577.
Front cover: Highly Dispersible and Bioavailable Curcumin but not Native Curcumin Induces Brown‐Like Adipocyte Formation in Mice 下载免费PDF全文
578.
Yukito Saitoh Yuji Yoshida Hiroyuki Kamiya 《Journal of the Society for Information Display》1999,7(2):115-118
Abstract— A 53° twisted-nematic cell for a color reflective liquid-crystal display was developed. It has a mirror electrode inside the panel on the TFT substrate, a twisted-nematic alignment structure, an RGB color filter, a single polarizer, and a light-control film covering the panel. Its advantages include gray-scale capability, low driving voltage, and a wide viewing angle. We discuss the Δn?d, the twist angle, and the front-light control film. 相似文献
579.
580.
Peng Lu Miaomiao Sui Mimin Zhang Mengyao Wang Takehiro Kamiya Ken Okamoto Hideaki Itoh Suguru Okuda Michio Suzuki Tomiko Asakura Toru Fujiwara Koji Nagata 《International journal of molecular sciences》2021,22(23)
Background: New strategies are needed to combat multidrug-resistant bacteria. The restriction of iron uptake by bacteria is a promising way to inhibit their growth. We aimed to suppress the growth of Vibrio bacterial species by inhibiting their ferric ion-binding protein (FbpA) using food components. Methods: Twenty spices were selected for the screening of FbpA inhibitors. The candidate was applied to antibacterial tests, and the mechanism was further studied. Results: An active compound, rosmarinic acid (RA), was screened out. RA binds competitively and more tightly than Fe3+ to VmFbpA, the FbpA from V. metschnikovii, with apparent KD values of 8 μM vs. 17 μM. Moreover, RA can inhibit the growth of V. metschnikovii to one-third of the control at 1000 μM. Interestingly, sodium citrate (SC) enhances the growth inhibition effect of RA, although SC only does not inhibit the growth. The combination of RA/SC completely inhibits the growth of not only V. metschnikovii at 100/100 μM but also the vibriosis-causative pathogens V. vulnificus and V. parahaemolyticus, at 100/100 and 1000/100 μM, respectively. However, RA/SC does not affect the growth of Escherichia coli. Conclusions: RA/SC is a potential bacteriostatic agent against Vibrio species while causing little damage to indigenous gastrointestinal bacteria. 相似文献