Specific Heat of the Surface Layers on the Amorphous Substrate in Solid and Liquid 3He

We have observed an excess heat capacity in liquid and bcc solid ³He at low temperatures. The heat capacity in the normal fluid is found to be the sum of the heat capacity of bulk normal fluid and a temperature-independent heat capacity C due to amorphous solid layers on the silver sinter surface, where C=7.3±6.8 JK^–1m^–2 corresponds to 1.6±0.6 amorphous solid layers. The heat capacity for bcc solid ³He is the sum of the heat capacity originating from the multiple-exchange interaction and a temperature independent heat capacity. The excess heat capacity C=12.1±3.1 JK^–1m^–2 for bcc solid corresponds to 1.9±0.3 amorphous solid layers. Our result indicates that the amorphous solid layers on an amorphous substrate yield a universal C in unit area throughout liquid, solid and adsorbed ³He.     

Performance Improvement of Current Source Inverter-Fed Induction Motor Drives

A high performance variable speed drive system using current source inverter fed induction motor is described. The principle of the drive control is based on the field-orientation method, where the stator current is used as the vector quantity. To improve the system response and to operate smoothly at low speed, a current pulsewidth modulation (PWM) control is provided by means of a feedback loop. This system operates stably and shows a high dynamic performance in a wide range, from zero to rated speed. Dual current source inverter is applied to this drive system which results in a remarkably low copper loss of the motor, even in PWM operation.     

A decentralized control system for cooperative transportation by multiple non-holonomic mobile robots

In this paper, we propose a decentralized control system for transporting a single object by multiple non-holonomic mobile robots. Each agent used in the proposed system has two arms, which can steer around a joint offset from the centre point between two driving wheels. One of these mobile robots acts as a leader, who is assumed to be able to plan and to manipulate the omnidirectional motion of the object by using a resolved velocity control. Other robots, referred to as followers, cooperatively transport the object by keeping a constant relative position with the object using a simple PI control. Different from conventional leader–follower type systems that transport an object by multiple robots in coordination, the present followers can plan an action based on their local coordinate and need no absolute positional information. In addition, as a special case, a system consisting of only two robots is introduced, in which the follower robot not only has an arm length controller to follow the leader but also has a fuzzy controller as an avoidance controller to avoid obstacles or a posture controller to keep a desired posture of the object. Simulation results are given to demonstrate the good performance of the proposed systems.     

Lateral Inhomogeneity in the Electronic Structure of a Conjugated Poly(3‐hexylthiophene) Thin Film

How annealing influences the morphology of a highly regioregular poly(3‐hexylthiophene) (RR‐P3HT) film at the substrate interface as well as the lateral inhomogeneity in the electronic structure of the film are elucidated. Whereas previous studies have reported that high‐molecular‐weight (MW) RR‐P3HT films tend to show low crystallinity even after annealing, it is found that high‐MW RR‐P3HT does show high crystallinity after annealing at high temperature for a long time. Photoemission electron microscopy (PEEM), X‐ray photoemission spectroscopy, and ultraviolet photoemission spectroscopy results clearly resolve a considerable lateral inhomogeneity in the morphology of RR‐P3HT film, which results in a variation of the electronic structure depending on the local crystallinity. The PEEM results show how annealing facilitates crystal growth in a high‐MW RR‐P3HT film.     

Reactive extraction of diols with phenyl boronic acid and trioctylmethylammonium chloride as coextractants and quantitative structure–property relationship of their extraction behaviors

BACKGROUND: Diols that can be produced biologically have attracted much attention because of the increased cost of producing them chemically. The cost of separating the diols from the broth forms a major part of the total cost of microbial production. Reactive extraction using organoboronate is one promising method for recovering diols from the dilute aqueous solution. RESULTS: A basic investigation of solvent extraction of diols was conducted at 303 K employing phenylboronic acid and trioctylmethylammonium chloride as coextractants in the mixed solvent. Both the tetrahedral boronate anion complex and trigonal boronate neutral complex were extracted. 1,3‐diols and vicinal diols were extracted, but 1,4‐diol was not extracted. Extraction equilibrium constants were correlated with the enthalpies of formation of the complexes, which were calculated by molecular modeling with semi‐empirical molecular orbital calculations considering the solvent effect. CONCLUSION: The complex extraction behaviour of diols with phenylboronic acid and quaternary ammonium salt can be predicted by using the quantitative structure–property relationship (QSPR). Copyright © 2009 Society of Chemical Industry     

Characteristics of surface roughening during plane strain compression of polycrystalline iron

Roughening behavior of the free surface of polycrystalline iron during plane strain compression is investigated experimentally. The changes in the shape of the free surface, which is roughened during plastic deformation, are observed in the three-dimensions. It is found that the mountains and the valleys of the roughened shape tend to elongate in the constrained direction for the specimen with isotropic grain shape. The shapes of the roughness curves in the loading direction and in the constrained direction are compared. The normalized height distribution of the roughness curve in the constrained direction is symmetric, while that of the roughness curve in the loading direction is asymmetric and positively skew during plane strain compression. Based on a simple simulation of the roughness curves by a random midpoint displacement method, this difference is supposed to be caused by the constraint of the material flow under plane strain condition.     

Liposome‐Based in Vitro Evolution of Aminoacyl‐tRNA Synthetase for Enhanced Pyrrolysine Derivative Incorporation

Methanosarcina species pyrrolysyl‐tRNA synthetase (PylRS) attaches Pyl to its cognate amber suppressor tRNA. The introduction of two mutations (Y384F and Y306A) into PylRS was previously shown to generate a mutant, designated LysZ‐RS, that was able to attach N‐benzyloxycarbonyl‐L ‐lysine (LysZ) to its cognate tRNA. Despite the potential of LysZ derivatives, further LysZ‐RS engineering has not been performed; consequently, we aimed to generate LysZ‐RS mutants with improved LysZ incorporation activity through in vitro directed evolution. Using a liposome‐based in vitro compartmentalization (IVC) approach, we screened a randomly mutagenized gene library of LysZ‐RS and obtained a mutant that showed increased LysZ incorporation activity both in vitro and in vivo. The ease and high flexibility of liposome‐based IVC should enable the evolution of not only LysZ‐RS that can attach various LysZ derivatives but also of other enzymes involved in protein translation.     

Supramolecular Ligands for Histone Tails by Employing a Multivalent Display of Trisulfonated Calix[4]arenes

Post‐translational modification of histone tails plays critical roles in gene regulation. Thus, molecules recognizing histone tails and controlling their epigenetic modification are desirable as biochemical tools to elucidate regulatory mechanisms. There are, however, only a few synthetic ligands that bind to histone tails with substantial affinity. We report CA2 and CA3, which exhibited sub‐micromolar affinity to histone tails (especially tails with a trimethylated lysine). Multivalent display of trisulfonated calix[4]arene was important for strong binding. CA2 was applicable not only to synthetic tail peptides but also to endogenous histone proteins, and was successfully used to pull‐down endogenous histones from nuclear extract. These findings indicate the utility of these supramolecular ligands as biochemical tools for studying chromatin regulator protein and as a targeting motif in ligand‐directed catalysis to control epigenetic modifications.     

Purification, characterization, and steady-state kinetics of a meta-cleavage compound hydrolase from Pseudomonas fluorescens IPO1

2-Hydroxy-6-oxo-7-methylocta-2,4-dienoate (6-isopropyl-HODA) hydrolase (CumD), an enzyme of the cumene biodegradation pathway encoded by the cumD gene of Pseudomonas fluorescens IP01, was purified to homogeneity from an overexpressing Escherichia coli strain. SDS-polyacrylamide gel electrophoresis and gel filtration demonstrated that it is a dimeric enzyme with a subunit molecular mass of 32 kDa. The pH optima for activity and stability were 8.0 and 7.0-9.0, respectively. The enzyme exhibited a biphasic Arrhenius plot of catalysis with two characteristic energies of activation with a break point at 20 degrees C. The enzyme has a K(m) of 7.3 microM and a k(cat) of 21 s(-1) for 6-isopropyl-HODA (150 mM phosphate, pH 7.5, 25 degrees C), and its substrate specificity covers larger C6 substituents compared with another monoalkylbenzene hydrolase, TodR Unlike TodF, CumD could slightly hydrolyze 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (6-phenyl-HODA). A mutant enzyme as to a putative active site residue, S103A, had 10(5)-fold lower activity than that of the wild-type enzyme.     

Field instrument for simultaneous large dynamic range measurement of atmospheric hydrogen sulfide, methanethiol, and sulfur dioxide

We describe a membrane-based collection/analysis system that differentially monitors H2S and CH3SH, and to which a conductometric SO2 analyzer using the same collector was coupled. A diffusion scrubber (DS) comprised of a Nafion tube collects H2S selectively while a porous polytetrafluoroethylene (pPTFE) DS collects both H2S and CH3SH. Both gases are measured via their ability to react with fluorescein mercuric acetate (FMA) which results in decreased fluorescence. The limited dynamic range of a negative signal procedure was overcome by using dual DS units comprised of short and long scrubbers, placed serially in the liquid flow line. Different DS designs and membrane materials were investigated. H2S, CH3SH, and SO2 from a biogenic point source were continuously measured, and the H2S/CH3SH data compared well with a standard procedure involving Tedlar bag collection, preconcentration and thermal desorption from a Tenax trap, and measurement by gas chromatography/flame photometric detection. Walkaround portability of the instrument and very large dynamic range measurement of H2S and SO2 were demonstrated around the Mt. Aso volcano.