Superantigenic exotoxin production by isolates of Staphylococcus aureus from the Kawasaki syndrome patients and age-matched control children

Ten screw-shaped commercially pure titanium implants were inserted in 10 rabbit tibia. The implants with surrounding bone were harvested after 12 weeks. The samples were processed to be cut and ground. Histomorphometrical analyses of the bone-to-metal contact and the bone area around the entire implants were performed on transvertically cut sections at intervals, starting with 100 microns thick sections; then the same sections were further ground down to 50, 30 and 10 microns. The thicker the section the more bone-to-metal contacts were demonstrated. A statistically significant difference was obtained when comparing the 100-30, 100-10, 50-30 and the 50-10 microns thick sections. Comparing the area measurements of 100-10, 50-30, 50-10 and 30-10 microns revealed a statistically significant difference. Bone-to-metal contact measurements on ground sections that are too thick (over 30 microns) may result in overestimations of the 'true' bony contacts.     

Comparative study of bone mineral density estimated by various methods of single- and dual-energy quantitative computed tomography: the capability of the four-equation four-unknown method

A dual-energy (DE) quantitative computed tomography (QCT) method, the four-equation four-unknown method (DEQCT 4E-4U), was assessed and compared to single-energy (SE) QCT and standard DEQCT (two-line method). The results of this study indicate that bone mineral density (BMD) was more accurately estimated by the present method than by the SEQCT or standard DEQCT techniques on the basis of a phantom study when a large fat content was present. The results of both the phantom study and a human study also showed that the present method corrected for fat in estimating BMD in the presence of high-fat content. These findings suggest that use of this method for estimating BMD can provide useful information in studies assessing the metabolic state of bone. We propose that CT numbers estimated from excised vertebral bone marrow can serve as a soft-tissue correction for the present method.     

Inhibition of chemotactic motility and trans-endothelial migration of human neutrophils by sphingosine 1-phosphate

In previous studies, we reported that sphingosine 1-phosphate (Sph-1-P) inhibits the chemotactic motility of some cancer cell lines such as mouse melanoma cells, as well as human smooth muscle cells, at a very low concentration, as demonstrated by a transwell migration assay method (Proc. Natl. Acad. Sci. USA 89, 9698, 1992; J. Cell Biol. 130, 193, 1995). In this study, we investigated the effect of Sph-1-P on the chemotactic motility and invasiveness of human neutrophils, utilizing three different assay systems: (a) a transwell migration assay where IL-8 or fLMP was added as a chemotactic factor, (b) a phagokinetic assay with gold colloids, and (c) a trans-endothelial migration assay with human umbilical vein endothelial cells (HUVECs) plated on collagen layers. We found that among various sphingosine derivatives, Sph-1-P specifically inhibited the IL-8- or fLMP-induced chemotactic migration of neutrophils at concentrations below 1 microM. Phagokinetic activity of neutrophils was also suppressed by Sph-1-P, but more moderately than by the PKC inhibitory sphingosine analog, trimethylsphingosine. Finally, Sph-1-P inhibited trans-endothelial migration and invasiveness of neutrophils into HUVEC-covered collagen layers, whereas no effect on their adhesion to HUVECs was observed. These observations strongly suggest that Sph-1-P can act as a specific and effective motility regulator of human neutrophils, raising the possibility of future applications of Sph-1-P, or its analogs, as anti-inflammatory agents regulating invasive migration of neutrophils through endothelial layers at injured vascular sites.     

Potent inactivator of alpha-chymotrypsin: 2,2-dimethyl-3-(N-4-cyanobenzoyl)amino-5-phenyl pentanoic anhydride

We synthesized a novel potent alpha-chymotrypsin inactivator, 2,2-dimethyl-3-(N-4-cyanobenzoyl) amino-5-phenyl pentanoic anhydride, which fulfilled the criteria of a mechanism-based inactivator: first-order kinetics, irreversibility, saturation kinetics and substrate protection. The inactivation rate constant (kinact) and the enzyme-inhibitor dissociation constant (KI) were calculated to be 0.017s-1 and 0.071 microM, respectively (kinact/KI = 242,000 M-1s-1). These kinetic parameters indicate that this compound is one of the most powerful alpha-chymotrypsin inactivators ever reported. The average number of alpha-chymotrypsin turnovers per inactivation (partition ratio) was calculated to be 1, which indicates that it is a stoichiometrically ideal inactivator of alpha-chymotrypsin. We compared the IC50 values of this compound with those of several chymotrypsin-like serine proteinases (bovine alpha-chymotrypsin, recombinant human chymase and human neutrophil cathepsin G) and a metallo proteinase, rabbit angiotensin converting enzyme (ACE). Our compound, 2,2-dimethyl-3-(N-4-cyanobenzoyl) amino-5-phenyl pentanoic anhydride, inhibited bovine alpha-chymotrypsin potently (IC50 = 1.0 (+/- 0.2) x 10(-9) M) as well as other chymotrypsin-like serine proteinase; recombinant human chymase (IC50 = 7.0 (+/- 1.0) x 10(-8) M) and human neutrophil cathepsin G (IC50 = 1.8 (+/- 0.2) x 10(-7) M). However, rabbit ACE was not inhibited by this compound (IC50 > 1 x 10(-4) M).     

An eight-bit prefetch circuit for high-bandwidth DRAM's

A low-power and area-efficient data path circuit for high-bandwidth DRAMs is described. For fast burst read operations, eight data per data I/O are stored in local latches placed close to sense amplifiers. As implemented in a 16-Mb synchronous DRAM (SDRAM), this 8-b prefetch circuit allows an early precharge command and a fast access time because it provides low-capacitance data lines for segmented bit-line pairs. At a column address strobe (CAS) latency of two and a burst length of four, the SDRAM demonstrates 100-MHz seamless read operations from different row addresses, because the row precharge and read access latencies are hidden during the burst cycles. The layout of the prefetch circuit is not limited by the bit-line pitch, and data path circuits are connected by a second-metal layer over the memory cells. As a result, a small chip size of 99.98 mm² is attained. Low-capacitance data lines and small local latches result in low active power. In a 100-MHz full-page burst mode, the SDRAM with a 1 M×16-b configuration dissipates 60 mA at 3.6 V     

Time lower bounds for parallel sorting on a mesh-connected processor array

Summary We prove that (1+6/2)n 2.22n is a time lower bound independent of indexing schemes for sorting n² items on an n × n mesh-connected processor array. We distinguish between indexing schemes by showing that there exists an indexing scheme which is provably worse than the snake-like row-major indexing for sorting. We also derive lower bounds for various indexing schemes. All these results are obtained by using the chain argument which we provide in this paper.A preliminary version of this paper was presented on the 3rd International Workshop on Parallel Computation and VLSI Theory, Corfu Island, Greece (June–July, 1988). Lect. Notes Comput. Sci. 319, 434–443 (1988)Research supported in part by the University of Kentucky research initiation award     

Knitting a 3D Model

A knitted animal is made of a closed surface consisting of several knitted patches knitted out of yarn and stuffed with cotton ( Fig. 1 ). We introduce a system to create a knitting pattern from a given 3D surface model (mainly designed for rotund animal models). A knitting pattern is an instructional diagram describing how to knit yarn to obtain a desired shape. Since the creation of knitting patterns requires special skill, this is difficult for nonprofessionals. Our system automates the process and allows anyone to obtain his or her original knitting patterns from a 3D model. The system first covers the surface of the model with parallel winding strips of constant width. The system then samples the strip at constant intervals to convert it into a knitting pattern. The result is presented in a standard visual format so that the user can easily refer it during actual knitting. We show several examples of knitted animals created using the system.

Figure 1 Open in figure viewer PowerPoint Example of a knitted animal. A typical knitted animal consists of several circular and cylindrical patches.