Comparison of the wound-activated transformation of caulerpenyne by invasive and noninvasive Caulerpa species of the Mediterranean

The invasive green alga, Caulerpa taxifolia, that has spread rapidly after its introduction into the Mediterranean and the North American Pacific, reacts to wounding by transforming its major metabolite caulerpenyne (1). This wound-activated reaction involves the transformation of the bis-enol acetate moiety of 1, releasing reactive 1,4-dialdehydes. The ability to perform this transformation is found also in both the noninvasive Mediterranean C. prolifera and the invasive C. racemosa. Trapping experiments, as well as transformation of the model substrate geranyl acetate, suggest that all three investigated Caulerpa spp. rely on esterases that act upon wounding of the algae by subsequently removing the three acetate residues of caulerpenyne. The resulting reactive 1,4-dialdehyde oxytoxin 2 (9) can be identified by liquid chromatography–mass spectrometry and is unstable in the wounded tissue. Caulerpenyne transformation occurs rapidly, and severe tissue damage caused degradation of more than 50% of the stored caulerpenyne within 1 min in all three algae. Prevention of the enzymatic reaction before extraction, by shock freezing the tissue with liquid nitrogen, was used for the determination of the caulerpenyne content in intact algae. It gives about twofold higher values compared to an established methanol extraction protocol. The speed and mechanism of the wound-activated transformation, as well as the caulerpenyne content in intact tissue of invasive and noninvasive Caulerpa spp., are comparable. Thus, this enzymatic , transformation, despite being fast and efficient, is likely not the key for the success of the investigated invasive species.     

Treatment of arsenic-containing solutions using chitosan derivatives: uptake mechanism and sorption performances

Modified chitosan gel beads, which had been prepared by the molybdate adsorption and coagulation (in the presence of molybdate) methods, were tested for As(III) and As(V) removal from dilute solutions (in the range 5-20 mg As L-1). The sorbent is very efficient at removing As(V) from acid solutions (optimum pH close to pH 2-3), whereas the sorption capacities are significantly lower for As(III) uptake (230 mg As(V) g-1 Mo, 70 mg As(III) g-1 Mo, respectively). Since the sorption proceeds in acidic solutions with a partial release of molybdate and with residual concentrations (ca. 500 micrograms As L-1) above the regulations for drinking water, the process appears to be directed to the treatment of industrial effluents or as a pre-concentration process. The mechanism of As(V) sorption is related to the ability of molybdate ions to complex As(V) ions in acid solutions. The uptake mechanism was confirmed by XPS analysis and desorption studies. In the case of As(III) sorption the mechanism of uptake is not identified since no complex has been cited in the literature regarding As(III) binding to Mo (VI), which was also identified by XPS analysis as the sorption site. As(V) sorption is not influenced by the presence of co-ions, with the exception of phosphate anions at low concentration, and silicate at high relative concentration. Arsenic desorption can be performed using phosphoric acid solutions.     

Long-time asymptotics of the Navier-Stokes and vorticity equations on R(3)

We use the vorticity formulation to study the long-time behaviour of solutions to the Navier-Stokes equation on R(3). We assume that the initial vorticity is small and decays algebraically at infinity. After introducing self-similar variables, we compute the long-time asymptotics of the rescaled vorticity equation up to second order. Each term in the asymptotics is a self-similar divergence-free vector field with Gaussian decay at infinity, and the coefficients in the expansion can be determined by solving a finite system of ordinary differential equations. As a consequence of our results, we are able to characterize the set of solutions for which the velocity field satisfies ||u(.,t)||(L(2)) = o(t(-5/4)) as t-->+ infinity. In particular, we show that these solutions lie on a smooth invariant submanifold of codimension 11 in our function space.     

Estimation of the total uncertainty of sky-radiance measurements in field experimental conditions: implications for the aerosol single-scattering albedo

We compare the spectral sky radiance measured by three ground-based optical radiometers during the second Aerosol Characterization Experiment (ACE-2) to estimate the total uncertainty of the radiance in field experimental conditions. The propagation of this uncertainty on the column-integrated aerosol single-scattering albedo omega0 at 868 nm is investigated. The radiance measurements are affected by a systematic gain uncertainty of less than 2% in the visible spectral region and within 6% in the near-IR region. Correcting the measured radiance by a systematic uncertainty reduces the dispersion of the omega0 from 0.07 to 0.03. Besides, the total relative uncertainty of the radiance measurements in field experimental conditions is within 4% at any wavelength. The corresponding uncertainty delta omega0 is 4% for an aerosol optical thickness of 0.2.     

Retrieval of the ultraviolet aerosol optical depth during a spring campaign in the Bavarian Alps

A measurement campaign was organized in March 1999 in the Bavarian Alps as part of the European project, Characteristics of the UV Radiation Field in the Alps (CUVRA), to analyze the effect of altitude, aerosols, and snow cover on ground-level UV spectral irradiance. We present the results of simultaneous measurements of aerosol optical depth (AOD) made at various sites on two cloudless days in March 1999. The two days exhibited different aerosol conditions. Results derived from spectral measurements of UV irradiance are compared with data from filter radiometer measurements made at discrete wavelengths extending from the UV to the near IR. The different methods generated values for the AOD that were in good agreement. This result confirms that one can use either method to retrieve the AOD with an uncertainty of approximately 0.03-0.05. On 18 March, high turbidity was observed at low altitude (400-nm AOD approximately 0.5 at 700 m above sea level), and the AOD decreased regularly with altitude; on 24 March, the turbidity was much less (0.11 at 700 m above sea level). On both days very low AODs (0.05-0.09) were measured at 3000 m above sea level. The spectral dependence of the AOD is often parameterized by the angstrom relationship; the alpha parameter is generally difficult or impossible to retrieve from spectral measurements because of the relatively narrow wavelength range (320-400 nm), and only one of the spectro-radiometers used during the campaign permits this retrieval. In most cases, during this field campaign, alpha was found by filter sunphotometers to be 1.1-1.5.     

Classification using belief functions: relationship between case-based and model-based approaches.

The transferable belief model (TBM) is a model to represent quantified uncertainties based on belief functions, unrelated to any underlying probability model. In this framework, two main approaches to pattern classification have been developed: the TBM model-based classifier, relying on the general Bayesian theorem (GBT), and the TBM case-based classifier, built on the concept of similarity of a pattern to be classified with training patterns. Until now, these two methods seemed unrelated, and their connection with standard classification methods was unclear. This paper shows that both methods actually proceed from the same underlying principle, i.e., the GBT, and that they essentially differ by the nature of the assumed available information. This paper also shows that both methods collapse to a kernel rule in the case of precise and categorical learning data and for certain initial assumptions, and a simple relationship between basic belief assignments produced by the two methods is exhibited in a special case. These results shed new light on the issues of classification and supervised learning in the TBM. They also suggest new research directions and may help users in selecting the most appropriate method for each particular application, depending on the nature of the information at hand.     

TGV: theory, principles and algorithms

This paper presents the TGV tool, which allows for the automatic synthesis of conformance test cases from a formal specification of a (non-deterministic) reactive system. TGV was developed by Irisa Rennes and Verimag Grenoble, with the support of the Vasy team of Inria Rhônes-Alpes. The paper describes the main elements of the underlying testing theory, which is based on a model of transitions system which distinguishes inputs, outputs and internal actions, and is based on the concept of conformance relation. The principles of the test synthesis process, as well as the main algorithms, are explained. We then describe the main characteristics of the TGV tool and refer to some industrial experiments that have been conducted to validate the approach. As a conclusion, we describe some ongoing work on test synthesis.     

Effect of Phenolic Acids in Soil under and Between Rows of a Prior Sorghum (Sorghum bicolor) Crop on Germination, Emergence, and Seedling Growth of Peanut (Arachis hypogea)

Sorghum bicolor is an allelopathic crop that reduces the yield of succeeding crops. We have assessed its effect on the germination, emergence, and seedling growth of Arachis hypogea sown in soil that had had a prior sorghum cropping. A. hypogea was sown on rows and interrows of a previous sorghum crop in 1997 and 1998 in Senegal. Seedling establishment (germination rate and seedling weight) was better between rows than on rows of the previous crop. The highest concentrations of phenolic compounds occurred in the rows in 1998, while contents of row and interrow soils were similar in 1997. Vanillic acid was the main component of the six chemicals found in 1997 soils, whereas the 1998 soil samples contained mainly p-hydroxybenzoic acid, p-hydroxybenzaldehyde, vanillic, and p-coumaric acids (10 phenolics identified). The germination of peanut seeds in water (control), soil water extracts, and mixtures of pure phenolics (equivalent to those in 1997 and 1998 soil samples) was tested. All extracts inhibited germination compared to controls, but there was no significant difference among treatments, i.e., the inhibition was the same for seeds in soil solutions and those in the respective phenolic mixtures. Similarly, there were no significant differences among the germination rates in soil water extracts of rows and interrows or in the pure phenolic mixtures of rows and interrows. We propose a geometrical sowing pattern for peanuts between the rows of the previous sorghum crop to escape the latter's "allelopathic heritage."     

Neutron Scattering from the Flux Line Lattice in the Cubic (K, Ba)BiO 3 Superconductor

Small Angle Neutron Scattering has been used to observe diffraction from the flux line lattice in the fully isotropic (K, Ba)BiO ₃ high T _c superconductor. We show that the flux lines are ordered into a hexagonal lattice at low field (H < H _e 0.6T H _g (0) where H _g is the second order vortex glass transition field). The diffracted intensity continuously drops to zero as the field is increased towards H _e . Those observations are in good agreement with recent theoritical works which suggested that the formation of dislocations becomes favorable above H _e leading to a glassy structure. The field H _e lies close to the onset of the second peak in magnetization measurements indicating that this peak is related to the change in the vortex structure.     

A new method to extract gate coupling ratio and oxide trapped charge in flash memory cell

A new method to obtain the gate coupling ratio (α_g) and oxide trapped charge (Q_ox) as a result of cycling in flash memory cells is described here. Three cells with an equivalent physical structure but different erase characteristics are measured. The threshold changes versus erase times are fitted to the charge removal rate calculated based on Fowler-Nordheim (FN) tunneling and the capacitive relations among all nodes. The extracted α_g is independent of technologies and this method is particular useful when the profile of the floating gate is not traditionally rectangular owing to advanced processes such as trapezoidal poly etch or a poly-spacer addition on the floating gate sidewall. The Q_ox can also be determined once α_g is extracted.