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11.
Natsuko Ota Fumihide Takano Shouta Muroga Tetsuro Kawabata Yasuhito Ishigaki Nobuo Yahagi Tomihisa Ohta 《Journal of Functional Foods》2012,4(1):243-252
The effects of garlic extract and three organosulphur compounds of garlic on intestinal immune responses in mice were investigated. Peyer’s patch (PP) cells were isolated from mice orally administered with garlic extracts or one of three organosulphur compounds (alliin, allicin, diallyl disulphide (DADS)). PP cells isolated from mice that had been orally injected with ethanol extract significantly produced interferon (IFN)-γ and interleukin (IL)-4. IL-2 production in PP cells was significantly reduced by hot-water and ethanol extracts from garlic. PP cells from mice administered with two organosulphur compounds, alliin or DADS (5 mg/kg/day), could produce IL-2, IFN-γ and IL-4, whereas allicin showed moderate activity. The enhancement activity of IL-2 and IFN-γ productions in PP cells by DADS was higher than those obtained by administration of alliin or allicin. Comprehensive analyses of genetic profiles in PP tissue from mice administered with ethanolic extracts, allicin or alliin revealed that oral administration of samples increased 68–144 genes and decreased 50–52 genes by ?1.8-fold. Analyses of clustering profiles of microarrays indicated that ethanol extract and alliin upregulated the expression of IFN-γ. These data showed that garlic and its organosulphur compounds stimulate de novo IFN-γ biosynthesis in PP cells, thereby promoting ileal immune responses. 相似文献
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Three kinds of polyamides were synthesized from three diamines and 1,3,5‐benzenetricarbonyl trichloride (TMC). The diamines used were m‐phenylene diamine, N‐methyl‐m‐phenylenediamine, and N,N′‐dimethyl‐m‐phenylenediamine. The average free volume sizes of the polyamides were measured by positron annihilation lifetime spectroscopy (PALS), and the free volume fractions were evaluated by molecular dynamics (MD) simulations. The methyl substitution on amino groups of diamines brought about an increase in interstitial space of molecular chains of the polyamides. In addition, reverse osmosis (RO) membranes were prepared by interfacial polymerization from the three diamines and TMC. The increase in the degree of methyl‐substitution of diamines led to increased chlorine resistance and decreased salt rejections of the polyamide RO membranes. Thus, the methyl‐substitution of diamines significantly influenced membrane performance. The vacancy sizes and fractional volumes in polyamides evaluated by PALS measurement and MD simulation were well correlated with salt rejection of polyamide RO membranes. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009 相似文献
14.
Ishiwata S. Yamakage T. Tsuboi Y. Shimazawa T. Kitazawa T. Michinaka S. Yahagi K. Takeda H. Oue A. Kodama T. Matsumoto N. Kamei T. Saito M. Miyamori T. Ootomo G. Matsui M. 《Solid-State Circuits, IEEE Journal of》2003,38(3):530-540
A single-chip MPEG-2 MP@ML codec, integrating 3.8M gates on a 72-mm/sup 2/ die, is described. The codec employs a heterogeneous multiprocessor architecture in which six microprocessors with the same instruction set but different customization execute specific tasks such as video and audio concurrently. The microprocessor, developed for digital media processing, provides various extensions such as a very-long-instruction-word coprocessor, digital signal processor instructions, and hardware engines. Making full use of the extensions and optimizing the architecture of each microprocessor based upon the nature of specific tasks, the chip can execute not only MPEG-2 MP@ML video/audio/system encoding and decoding concurrently, but also MPEG-2 MP@HL decoding in real time. 相似文献
15.
M Ichinose N Yahagi Y Matsubara S Tsukada M Oka Y Shimizu S Yonezawa T Kageyama K Miki H Fukamachi 《Canadian Metallurgical Quarterly》1997,230(3):537-541
A system for the primary serum-free culture of fetal rat gastrointestinal epithelial cells was used to examine the role of the extracellular matrix (ECM) in the attachment and proliferation of these epithelial cells. Forestomach epithelial cells (FSEC) were able to attach to and proliferate on plastic dishes without a substratum, while glandular stomach epithelial cells (GSEC) and duodenal epithelial cells (DEC) were unable to do so. The presence of a substratum promoted the attachment and proliferation of these epithelial cells. The effects of various components of the ECM differed depending on the type of cell. FSEC attached most efficiently to a substratum of fibronectin, while GSEC did so to laminin. DEC attached more efficiently to type I collagen and fibronectin than to any other substratum. FSEC proliferated most rapidly on laminin, while GSEC and DEC did so on collagen gels. These substrata induced the most efficient attachment and proliferation of FSEC, and they were effective in promoting the attachment and proliferation of GSEC and DEC in decreasing order of efficiency, indicating the existence of a head-to-tail gradient in the response of epithelial cells to substrata. The expression of c-myc mRNA in these cells differed depending upon the substratum on which they were cultured and the mRNA level was well correlated with the extent of the cell proliferation, indicating that the cell proliferation is mediated by c-myc gene expression, which is regulated by cell-ECM interactions. The results of the present study demonstrate that proliferation of gastrointestinal epithelial cells is regulated region-specifically not only by soluble factors but also by insoluble components of the ECM. 相似文献
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A Method of Face Recognition Based on Fuzzy c-Means Clustering and Associated Sub-NNs 总被引:1,自引:0,他引:1
The face is a complex multidimensional visual model and developing a computational model for face recognition is difficult. In this paper, we present a method for face recognition based on parallel neural networks. Neural networks (NNs) have been widely used in various fields. However, the computing efficiency decreases rapidly if the scale of the NN increases. In this paper, a new method of face recognition based on fuzzy clustering and parallel NNs is proposed. The face patterns are divided into several small-scale neural networks based on fuzzy clustering and they are combined to obtain the recognition result. In particular, the proposed method achieved a 98.75% recognition accuracy for 240 patterns of 20 registrants and a 99.58% rejection rate for 240 patterns of 20 nonregistrants. Experimental results show that the performance of our new face-recognition method is better than those of the backpropagation NN (BPNN) system, the hard c-means (HCM) and parallel NNs system, and the pattern-matching system 相似文献
18.
Sekiya H. Nemoto S. Jianming Lu Yahagi T. 《IEEE transactions on circuits and systems. I, Regular papers》2006,53(2):254-263
In this paper, a phase control scheme for Class-DE-E dc-dc converter is proposed and its performance is clarified. The proposed circuit is composed of phase-controlled Class-DE inverter and Class-E rectifier. The proposed circuit achieves the fixed frequency control without frequency harmonics lower than the switching frequency. Moreover, it is possible to achieve the continuous control in a wide range of the line and load variations. The output voltage decreases in proportion to the increase of the phase shift. The proposed converter keeps the advantages of Class-DE-E dc-dc converter, namely, a high power conversion efficiency under a high-frequency operation and low switch-voltage stress. Especially, high power conversion efficiency can be kept for narrow range control. We present numerical calculations for the design and the numerical analyses to clarify the characteristics of the proposed control. By carrying out circuit experiments, we show a quantitative similarity between the numerical predictions and the experimental results. In our experiments, the measured efficiency is over 84% with 2.5 W output power for 1.0-MHz operating frequency at the nominal operation. Moreover, the output voltage is regulated from 100% to 39%, keeping over 57% power conversion efficiency by using the proposed control scheme. 相似文献
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S Tsukada M Ichinose N Yahagi Y Matsubara S Yonezawa K Shiokawa C Furihata K Miki H Fukamachi 《Canadian Metallurgical Quarterly》1998,62(5):239-247
Activation of prothrombin by multisquamase, the prothrombin activator from the venom of Echis multisquamatus (Central Asian sand viper), is inhibited by membranes containing negatively charged anionic phospholipids. This inhibition appears to be due to the fact that the venom activator cannot activate membrane-bound prothrombin. Initial steady state rates of prothrombin activation by multisquamase in the presence of phospholipids appeared to depend on the fraction unbound prothrombin only and this phenomenon was used to quantitate binding of prothrombin to membranes of varying phospholipid composition. In this method, the initial rate of prothrombin activation by multisquamase is measured in the absence (total prothrombin) and in the presence of a procoagulant surface (rate depending only on free prothrombin) and from the difference in activation rates the amount of membrane-bound prothrombin is calculated. The validity of the method was established by determination of the binding parameters for prothrombin binding to 100 microM phospholipid vesicles composed of 20 mole% phosphatidylserine and 80 mole% phosphatidylcholine. The binding parameters obtained were Kd=0.84 microM and n=0.021 micromoles prothrombin bound per micromole phospholipid which is in agreement with literature. Due to the nature of the measurement the method is especially suitable to quantitate binding of prothrombin at concentrations as low as 5 nM prothrombin. 相似文献