Blood speed imaging with an intraluminal array

In applications in which Doppler processing is not possible, such as side-looking intravascular imaging systems, decorrelation methods can be used to estimate blood speed. Here, a method is presented measuring relative blood speed using an FIR filter bank to estimate temporal decorrelation rates. It can be implemented in a modern commercially available ultrasound imaging system with no additional hardware. Both simulations and experiments using an intraluminal scanner appropriate for coronary artery applications have tested the system. In this study, the FIR filter bank is contrasted with previous methods, and its utility is further demonstrated with real-time color flow images from a pig model.     

Vectorcardiographic loop alignment and the measurement of morphologic beat-to-beat variability in noisy signals

The measurement of subtle morphologic beat-to-beat variability in the electrocardiogram (ECG)/vectorcardiogram (VCG) is complicated by the presence of noise which is caused by, e.g., respiration and muscular activity. A method was recently presented which reduces the influence of such noise by performing spatial and temporal alignment of VCG loops. The alignment is performed in terms of scaling, rotation and time synchronization of the loops. Using an ECG simulation model based on propagation of action potentials in cardiac tissue, the ability of the method to separate morphologic variability of physiological origin from respiratory activity was studied. Morphologic variability was created by introducing a random variation in action potential propagation between different compartments. The results indicate that the separation of these two activities can be done accurately at low to moderate noise levels (less than 10 microV). At high noise levels, the estimation of the rotation angles was found to break down in an abrupt manner. It was also shown that the breakdown noise level is strongly dependent on loop morphology; a planar loop corresponds to a lower breakdown noise level than does a nonplanar loop.     

Analysis of a sleep-dependent neuronal feedback loop: the slow-wave microcontinuity of the EEG

Increasing depth of sleep corresponds to an increasing gain in the neuronal feedback loops that generate the low-frequency (slow-wave) electroencephalogram (EEG). We derived the maximum-likelihood estimator of the feedback gain and applied it to quantify sleep depth. The estimator computes the fraction (0%-100%) of the current slow wave which continues in the near-future (0.02 s later) EEG. Therefore, this percentage was dubbed slow-wave microcontinuity (SW%). It is not affected by anatomical parameters such as skull thickness, which can considerably bias the commonly used slow-wave power (SWP). In our study, both of the estimators SW% and SWP were monitored throughout two nights in 22 subjects. Each subject took temazepam (a benzodiazepine) on one of the two nights. Both estimators detected the effects of age, temazepam, and time of night on sleep. Females were found to have twice the SWP of males, but no gender effect on SW% was found. This confirms earlier reports that gender affects SWP but not sleep depth. Subjectively assessed differences in sleep quality between the nights were correlated to differences in SW%, not in SWP. These results demonstrate that slow-wave microcontinuity, being based on a physiological model of sleep, reflects sleep depth more closely than SWP does.     

Micro-emboli detection: an ultrasound Doppler signal processing viewpoint

Several studies have been carried out in the last twenty years on the characterization and detection of cerebral artery emboli. From the detection point of view, the existing methods are largely based on the classical Fourier analysis of which the well known limitations provide poor accuracy. This paper first recalls existing methods based on Fourier, Wigner-Ville and wavelet approaches. It then presents new emboli detection methods based on parametric signal processing approaches. The basic idea of these parametric methods is to compare the Doppler embolic signal to its autoregressive model. The detection principle consists in constructing a decision information which contains the signature of the micro-embolus being sought. The detection is finally evaluated using receiver operating characteristic (ROC) curves. Comparison between the new methods and classical approaches is performed using a realistic embolic signal simulation. Furthermore, to validate our theoretical study, we tested our new algorithms using in vivo signals. This comparison shows the significant inaccuracy of existing methods to detect micro-emboli.     

Short communication: effect of tannic acid on composition and ruminal degradability of bermudagrass and alfalfa silages

We measured the effects of the addition of tannic acid on chemical composition and crude protein (CP) ruminal degradability of bermudagrass, cv. coast cross (Cynodon dactylon L.) and alfalfa (Medicago sativa L.) silages with nylon bags incubated in the rumen of two fistulated lactating cows. Silage pH was greater for alfalfa than for bermudagrass. The addition of tannic acid had no effect on silage pH. Ammonia N was greater in alfalfa than in bermudagrass silage. Treated and control silages had similar ammonia N concentrations. The percentage of CP that was solubilized at time 0 was lower for alfalfa than for bermudagrass silage. The addition of tannic acid decreased the portion of CP solubilized at time 0 and increased the potentially degradable fraction of CP. The potentially degradable fraction of CP was greater for alfalfa than for bermudagrass. There was an interaction of species and treatment; the disappearance rate of CP increased with tannic acid treatment of alfalfa but decreased with acid treatment of bermudagrass. The effective degradability of CP was similar for control and treated alfalfa but lower for treated than for control bermudagrass. Tannic acid treatment is effective in decreasing the rapidly soluble fraction of alfalfa and bermudagrass silages, which could be beneficial to the animal because it would decrease the excess of N in the rumen after feeding. Tannic acid treatment decreased CP degradability of bermudagrass silage but had no effect on alfalfa silage, suggesting that tannic acid concentration required to effectively decrease CP degradability differs among forages.     

Do glycerolipids display lateral heterogeneity in the thylakoid membrane?

The lateral heterogeneity of lipids in the thylakoid membrane has been questioned for over 20 yrs. It is generally believed that glycerolipids are asymmetrically distributed within the plane of the membrane. In the present investigation, we isolated several thylakoid membrane domains by using sonication followed by separation in an aqueous dextran-polyethylene glycol two-phase system. This technique, which avoids detergent treatments, allowed us to obtain stroma and grana lamellae vesicles as well as grana central core and grana margin vesicles from thylakoids. The relative distribution of the four lipid classes, i.e., monogalactosyldiacylglycerol, digalactosyldiacylglycerol, sulfoquinovosyldiacylglycerol, and phosphatidylglycerol, was found to be statistically identical in all four thylakoid fractions and in whole thylakoids. Similarly, the relative amount of fatty acids in each individual lipid and the eight main phosphatidylglycerol molecular species was identical in all thylakoid membrane fractions tested as well as in the intact thylakoid membrane. Based on presently available procedures for obtaining thylakoid subfractions that are unable to discriminate microdomains within the membrane, it is concluded that glycerolipids are evenly distributed within the plane of the thylakoid membrane. These data are discussed in terms of “bulk” and “specific” lipids.     

Evaluation of biohazards in dehydrated biofilms on foodstuff packaging

Plastic materials used for food packaging are clean but not sterile when the food is just packaged. Accidental wet contamination may occur at every moment between packaging and opening by the consumer: on polyethylene (PET), bacteria may adhere strongly and constitute a biofilm in less than 24 h. By rolling on themselves, PET sheets may contaminate food. We tried to show that contact with salted foodstuffs favoured microbial recovery. Four strains were chosen to perform biofilms on PET: Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli. Biofilms were dried up 24 h. Biofilm bacteria were stressed by adhesion, by starvation and by dehydration. However, they were capable of recovery in salted solutions or media, probably because one (or more) stress protected them against another stress. Stress was demonstrated by stress protein production, by mean of electrophoresis, and membrane lesions by mean of flow cytometry. Stress recovery was performed in aqueous salted solutions or salted brain-heart infusion with NaCl 9, 15, 20 and 30 g/l. Staphylococci were more sensitive to these stresses and recovery was a function of salt concentration. Gram-negative bacteria were little affected by stresses; salt effects were less important. If all these biofilms were capable of recovery from stresses in salted media, flexible PET could possibly lead to a health hazard when it is used for wet salt meats, e.g.     

Scanning electron microscopic observation on the equatorial region of the rat intrafusal muscle fibres, especially on the subsarcolemmal sarcoplasmic reticulum

We examined intracellular structures in the equatorial region of the muscle spindles of rat soleus muscles by scanning electron microscopy, paying particular attention to the ultrastructure of the sarcoplasmic reticulum (SR) beneath the sarcolemma. The subsarcolemmal SR was more developed in nuclear chain fibres than in nuclear bag fibres as was reported in the sleeve and extracapsular regions. In addition, the subsarcolemmal SR of the chain fibre formed a fenestrated sheet, whereas that of the bag fibre organized a layer of fenestrated bands beneath the sarcolemma where the sensory nerve endings are associated. No T-tubules were discerned in the subsarcolemmal SR of both fibres, which may be concerned with the little contraction of the equatorial region.