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51.
Activated endothelial cells and stimulated platelets express the cell adhesion molecule P-selectin (CD62P), which mediates adhesion to various leukocytes and certain types of cancer cells. In this study, we show Ca2+-dependent binding of P-selectin to NKI-4 cells, a cell line derived from a human melanoma. The binding is inhibited by P7 (a leukocyte adhesion blocking mAb against P-selectin), but not by PL5 (a leukocyte adhesion blocking mAb against P-selectin glycoprotein ligand-1; PSGL-1). Further, expression of PSGL-1 could not be detected on NKI-4 cells by either PL5 mAb or an Ab against a synthetic peptide corresponding to a portion of the PSGL-1 sequence. P-selectin affinity chromatography of lysates from in vivo [3H]-glucosamine-labeled NKI-4 cells resulted in the isolation of three glycoproteins, with apparent molecular masses of approximately 250, approximately 110, and approximately 100 kDa under reducing conditions and approximately 230, approximately 105, and approximately 85 kDa under nonreducing conditions. These molecules could be precipitated by P-selectin, but not by E-selectin. EDTA and the P7 mAb, but not the PL5 mAb, inhibited the binding of P-selectin to the purified ligands. Surprisingly, we found that sodium chlorate, a sulfation inhibitor, did not inhibit the binding of P-selectin to NKI-4 cells and that [35S]-sulfate did not label the NKI-4 cell ligands. We conclude that P-selectin-dependent adhesion of the human melanoma cell line NKI-4 is mediated by a novel class of glycoprotein ligands. 相似文献
52.
手绘服装动画是艺术化绘制风格与服装运动规律的结合体,将手绘风格与服装运动模拟相融合,提出一种高效生成具有手绘效果的服装动画方法.通过手绘图来指定服装动画的艺术效果,并基于逆向动力学原理,采用非线性优化方法从用户输入的手绘图中恢复出服装运动的动力学参数;在此基础上,设计实现了基于广义的中间帧插值方法和结合显式动作数据的外推方法,自动生成用户所期望的、与手绘图风格一致的服装动画序列.实验结果表明,该方法可有效地生成融合服装物理运动属性并保留动画师手绘风格的服装动画,显著提高了手绘服装动画的制作效率. 相似文献
53.
与传统单宗房地产评估不同,房地产批量评估需要建立在强大详实的数据库基础上,如何构建高可靠性、高扩展性以及高集成性的批量评估数据库成为一项关键任务。在对房地产批量评估数据进行分析的基础上,基于GIS技术提出了房地产批量评估数据库构建的技术路线,设计了数据库的总体结构,并研究了房地产批量评估数据集成的方法,最后以深圳市为例说明了该方法的有效性。 相似文献
54.
为了让不同组织在保护本地敏感数据和降维后发布数据隐私的前提下,联合使用PCA进行降维和数据发布,提出横向联邦PCA差分隐私数据发布算法。引入随机种子联合协商方案,在各站点之间以较少通信代价生成相同随机噪声矩阵。提出本地噪声均分方案,将均分噪声加在本地协方差矩阵上。一方面,保护本地数据隐私;另一方面,减少了噪声添加量,并且达到与中心化差分隐私PCA算法相同的噪声水平。理论分析表明,该算法满足差分隐私,保证了本地数据和发布数据的隐私性,较同类算法噪声添加量降低。实验从隐私性和可用性角度评估该算法,证明该算法与同类算法相比具有更高的可用性。 相似文献
55.
Gao Fang Jia Xiaofeng Zhao Zhiyun Chen Chih-Cheng Xu Feng Geng Zhe Song Xiaotong 《Microsystem Technologies》2021,27(4):1545-1557
Microsystem Technologies - Artificial intelligence (AI), together with its applications, has received world-wide attentions and is expected to exert force on the development of global economy and... 相似文献
56.
We introduce a novel method for synthesizing dance motions that follow the emotions and contents of a piece of music. Our method employs a learning-based approach to model the music to motion mapping relationship embodied in example dance motions along with those motions' accompanying background music. A key step in our method is to train a music to motion matching quality rating function through learning the music to motion mapping relationship exhibited in synchronized music and dance motion data, which were captured from professional human dance performance. To generate an optimal sequence of dance motion segments to match with a piece of music, we introduce a constraint-based dynamic programming procedure. This procedure considers both music to motion matching quality and visual smoothness of a resultant dance motion sequence. We also introduce a two-way evaluation strategy, coupled with a GPU-based implementation, through which we can execute the dynamic programming process in parallel, resulting in significant speedup. To evaluate the effectiveness of our method, we quantitatively compare the dance motions synthesized by our method with motion synthesis results by several peer methods using the motions captured from professional human dancers' performance as the gold standard. We also conducted several medium-scale user studies to explore how perceptually our dance motion synthesis method can outperform existing methods in synthesizing dance motions to match with a piece of music. These user studies produced very positive results on our music-driven dance motion synthesis experiments for several Asian dance genres, confirming the advantages of our method. 相似文献
57.
58.
步行街区作为城市公共空间的重要组成部分,其空间活力程度对城市经济与社会发展具有重要意义.为探讨街区空间品质对街区活力的影响效应及其影响程度,以合肥市淮河路步行街区为例,通过调研街区的建筑品质、景观品质、文化品质和管理维护品质,采用回归分析定量研究街区空间品质对街区活力的影响效应及程度.研究结果表明,街区空间的文化品质对街区活力的影响最大,景观品质其次,建筑和管理维护品质最小.未来进行城市步行街区设计时,应注重塑造街区的文化品质. 相似文献
59.
Cells employ proteins to perform metabolic functions and maintain active physiological state through charge transfer and energy conversion. These processes are carried out in a narrow space precisely and rapidly, which, no doubt, bring great difficulty for their detection and dissection. Fortunately, in recent years, the development and expansion of single-molecule technique in protein research make monitoring the dynamical changes of protein at single-molecule level a reality, which also provides a powerful tool for the further exploration of new phenomena and new mechanisms of life activities. This paper aims to summarize the working principle and essential achievements of single-molecule technique in protein research in recent five years. We focus on not only dissecting the difference of nanopores, atomic force microscope, scanning tunneling microscope, and optical tweezers technique, but also discussing the great significance of these single-molecule techniques in investigating intramolecular and intermolecular interactions, electron transport, and conformational changes. Finally, the opportunities and challenges of the single-molecule technique in protein research are discussed, which provide a new door for single-molecule protein research. 相似文献
60.
A multifunctional thiophene-based covalent triazine framework (TTPATTh) with triphenylamine as core is synthesized by Friedel–Crafts reaction of cyanuric chloride with thienyl derivative for the first time. The yield of TTPATTh (99.59%) is far higher than that of the carbazole-based CTF (TTPATCz, 47.03%). TTPATTh and TTPATCz possess high BET surface areas with 1235 and 2501 m2 g–1 as well as high pore volumes with 1.60 and 2.23 cm3 g–1, respectively. TTPATTh and TTPATCz have high thermal stability with high thermal decomposition temperatures of 514 and 598 °C in nitrogen atmosphere. With the introduction of triazine rings, the fluorescence sensing sensitivities of TTPATTh and TTPATCz to picric acid and iodine are improved significantly. Especially for TTPATTh, the Ksv values reach 5.95 × 105 and 1.61 × 104 L mol–1, and LODs reach 1.02 × 10–12 and 1.86 × 10–12 mol L–1. To the best of the knowledge, this is the most sensitive value among thiophene-based porous organic polymers to PA and I2. Furthermore, TTPATTh and TTPATCz can also adsorb iodine in vapor phase, cyclohexane solution and aqueous solution, and release iodine by heating or in ethanol solution. The efficiency of controlling the release of iodine is higher than that in ethanol. 相似文献