Differential cellular response within the rabbit tendon unit following tendon injury

Since 1975, 340 patients were treated by tracheal sleeve resection for tracheal or subglottic laryngeal iatrogenic stenoses in our unit. Preoperative iterative Nd YAG laser sessions have usually been performed, without success. The length of the sleeve specimen was an average of 3 1/4 cm. Twelve patients died on the post operative course (3.5%), 3 more patients died later after failure of the procedure (0.9%) and nineteen had recurrent stenoses treated with use of a tracheostomy tube, a permanent Montgomery tube, or an endotracheal stent (5.6%). Three hundred and six patients are definitely cured (90%), at the first attempt for 265 patients, after a laser session for granulomas for 20 patients, after a second tracheal resection for 6 patients and after a temporary Montgomery tube for 15 patients. Providing there is a good selection of the patients, tracheal sleeve resection is the best treatment for iatrogenic stenosis.     

Study of Lateral Spread of Ions Emitted from 2.3 kJ Plasma Focus with Hydrogen and Nitrogen Gases

The characteristics of ion beams of hydrogen and nitrogen with different filling pressures emitted from the plasma focus device of 2.3 kJ energy are investigated. CR-39 SSNTDs are employed for the registration of tracks of ions. The exposed detectors are etched in 6N NaOH solution at 70°C and then examined with an optical microscope. The ion flux is estimated to be of the order of 10^5–6 tracks/cm². The flux with the radial position does not exhibit any regular pattern of variation.     

Activation of large-conductance potassium channels in pregnant human myometrium by pinacidil

OBJECTIVE: The aim was to investigate the effects of the potassium-channel opener pinacidil on single uterine potassium channels and the contribution of the latter to pinacidil-induced myometrial relaxation. STUDY DESIGN: Myometrial strips and freshly dispersed uterine myocytes were prepared from the myometrial biopsy samples of women undergoing elective, nonlabor caesarean section at term gestation. RESULTS: In isometric tension experiments pinacidil potently relaxed pregnant nonlabor human myometrial strips, with an agonist concentration yielding the half maximal response of 0.4 +/- 0.1 micromol/L. This effect was antagonized by 500 nmol/L charybdotoxin. Application of 10 micromol/L glibenclamide also inhibited the pinacidil-induced relaxation. Coapplication of charybdotoxin (500 nmol/L) and glibenclamide (10 micromol/L) produced a biphasic curve, which was fitted to a two-site model with values for agonist concentration yielding the half maximal response of 0.6 +/- 0.2 micromol/L and 189.7 +/- 0.8 micromol/L. Large-conductance calcium-dependent potassium channel activity was dramatically increased after application of pinacidil (between 10 and 100 micromol/L) to both inside-out and outside-out patches. The activation required the presence of calcium ions at the intracellular aspect of the membrane. Charybdotoxin but not glibenclamide blocked pinacidil-induced unitary large-conductance calcium-dependent potassium channel activity. CONCLUSION: Pinacidil-mediated relaxation of human pregnant myometrial strips may be partially attributable to the opening of uterine large-conductance calcium-dependent potassium channels in addition to adenosine triphosphate potassium channel activation. Drugs with specific potassium channel-activating properties may have important clinical application as novel tocolytics in the treatment of preterm labor.     

Macrophage formation of angiostatin during inflammation. A byproduct of the activation of plasminogen

Angiostatin is a potent inhibitor of tumor angiogenesis and the growth of metastatic foci. Recent studies have indicated that neoplastic cells can generate angiostatin directly or in cooperation with tumor-associated macrophages. In studies reported here, we determined whether angiostatin is generated in mice under non-neoplastic settings. Utilizing murine RAW264.7 macrophages and thioglycollate-elicited peritoneal macrophages, we demonstrate that angiostatin-like fragments are generated as a byproduct of the proteolytic regulation of membrane-bound plasmin. Plasmin proteolysis and subsequent loss in membrane-bound plasmin activity requires active plasmin but was unaffected by inhibitors of metalloproteinases. Lysine binding fragments of plasmin, isolated from macrophage-conditioned media utilizing affinity chromatography, appeared as a major (48 kDa) and two minor bands (42 and 50 kDa) in SDS-polyacrylamide gel electrophoresis and were immunoreactive with anti-kringle 1-3 IgG. Each peptide begins with Lys77 and contains the entire sequence of angiostatin. The affinity isolated plasmin fragments inhibited bFGF-induced endothelial cell proliferation. Lavage fluid recovered from the peritoneal cavities of mice previously injected with thioglycollate contained angiostatin-like plasmin fragments similar to those generated in vitro. This is the first demonstration that angiostatin-like plasmin fragments are generated in a non-neoplastic inflammatory setting. Thus, in addition to regulating pericellular plasmin activity, proteolysis of plasmin generates inactive kringle-containing fragments expressing angiostatic properties.     

Effects of Inhibition of Carotenoids on the Lipids Synthesis by Micrococcus colpogenes

Effect of glucose concentration and the inhibition of carotenoids on the lipids synthesis by Micrococcus colpogenes were studied. It was observed that the maximum lipid production was obtained when 2 % glucose was used as carbon source. The inhibition of carotenoids with diphenylaminc (25 mg/L) showed 37 % increase in the lipid synthesis.     

Wireless transmission of HD video using H.264 compression over UWB channel

With the technological advancement, entertainment has become revolutionized and the high-definition (HD) video has become an integral feature of our modern amusement system. The demand for wireless transmission of HD video is rapidly rising for its ubiquitous nature, easy installation and relocation. Such wireless transmissions of HD video streams require very high bandwidth. The ultra-wideband (UWB) offers a large bandwidth, and short-range high-speed data transmission at low cost and low power consumption. In this paper, we present the feasibility study to transmit HD video wirelessly using H.264/AVC compression over the UWB communication channel. Simulations are carried out by controlling key H.264/AVC encoder parameters such as, in-loop deblocking filter, group of pictures, and quantization parameter. Based on the analysis, an optimum setting of these parameters is proposed for different bandwidth requirements, as well as acceptable video quality. The bandwidth achieved is restricted between 1.5 and 20?Mbps with a minimum reconstruction quality of 34?dB. The HD bit stream is then transmitted over the UWB communication channel and the demonstration shows that the overall encoder performance is satisfactory with the transmission bit-error-rate (BER) in the range of 10^?5?C10^?8.