Simultaneous determination of five antioxidants in food by HPLC with fluorescence detection

An HPLC method with fluorescence detection was developed for the determination of propyl gallate, nordihydroguaiaretic acid, butylated hydroxyanisole (2- and 3-tert-butyl-4-hydroxyanisole), tert-butylhydroquinone and octyl gallate in edible oils and foods. The antioxidants in edible oil were isolated directly with acetonitrile saturated with n-hexane. The antioxidants in food were extracted with ethyl acetate and the extract was concentrated under vacuum. They were isolated from the residue with acetonitrile saturated with n-hexane. The acetonitrile layer was centrifuged at 5,000 rpm for 10 min. The HPLC separation was performed on a Symmetry C18 column (3.5 microns, 4.6 mm i.d. x 150 mm) using a mixture of 5% acetic acid-acetonitrile-methanol (4:3:3, v/v/v) as the mobile phase and monitored by using a fluorescence detector with time programming. Sample peaks were identified by comparison of the fluorescence spectra with those of antioxidant standards. Average recoveries of fortified antioxidants at 100 micrograms/g were 72.1-99.6%. Coefficients of variation were 0.7-7.2%.     

Space–time SUPG finite element computation of shallow-water flows with moving shorelines

We show that combination of the Deforming-Spatial-Domain/Stabilized Space–Time and the Streamline-Upwind/Petrov–Galerkin formulations can be used quite effectively for computation of shallow-water flows with moving shorelines. The combined formulation is supplemented with a stabilization parameter that was originally introduced for compressible flows, a compressible-flow shock-capturing parameter adapted for shallow-water flows, and remeshing based on using a background mesh. We present a number of test computations and provide comparisons to theoretical results, experimental data and results computed with nonmoving meshes.     

Geothermal resources of Hachijojima

Hachijojima is a gourd-shaped volcanic island in the Pacific Ocean. Nishiyama and Higashiyama volcanoes consist of basalt lava and associated pyroclastic rocks. A promising geothermal resource was found in south Higashiyama, associated with an uplift of Tertiary rocks consisting of mainly andesite lava and related pyroclastic rocks, overlain by Quaternary volcanic rocks. Steep high-temperature (over 250°C) and high-pressure gradients occur in the deeper portion of the system near the Tertiary–Quaternary contact, indicating the presence of a cap rock. The cap rock formed by deposition of hydrothermal minerals. Geothermal fluid ascends from the deeper portions to shallow depths along vertical fractures through the cap rock. These vertical fractures form the geothermal reservoir in the Tertiary formation. Three wells were drilled into these vertical fractures, and approximately 30 t/h of superheated steam was obtained from each well during flow tests. The geothermal fluid is mainly a mixture of seawater and meteoric water in an approximate ratio of 1 to 2, based on chemical analyses, with a portion of volcanic gas included. At present a 3.3 MW_e, geothermal power plant is being constructed here.     

Heat Source in Infinite Plane with Elliptic Rigid Inclusion and Hole

The plane thermoelastic problems of a stationary heat source in an infinite plane with an elliptic rigid inclusion and an elliptic hole are analyzed under thermally adiabatic and isothermal boundary conditions. The problems of an elliptic rigid inclusion are derived for the following cases: (1) the case that there are rigid-body displacement and rotation; and (2) the case that there is no rigid-body displacement or rotation. To analyze these problems, the following three fundamental solutions are derived: Problem A, in which a point heat source exists within an infinite domain; Problem B, in which the inclusion has a small amount of rotation; and Problem C, in which the inclusion is subjected to concentrated loads. Two cases can be obtained by superimposing these fundamental solutions. For the hole problem, the fundamental solution (Green's function) is also derived. In analysis, the complex stress functions, the mapping function, and the thermal dislocation method are used. The complex stress functions are obtained as a closed form. For analytic examples, the stress distributions are shown under thermally adiabatic and isothermal boundary conditions. For the crack problem, the stress intensity factors are shown for the location of the heat source.     

Advanced energy saving in the reaction section of the hydro-desulfurization process with self-heat recuperation technology

The reaction section of the naphtha hydro-desulfurization (HDS) process is a heating and cooling thermal process consisting of a feed/effluent heat exchanger and a fired heater. Energy savings are fundamentally made as a result of the maximized heat recovery in the heat exchanger and the reduced heat duty of the fired heater. To achieve further energy saving in the process, “self-heat recuperation technology” (SHRT) was adopted. In this technology, a compressor was introduced. The suction side of the compressor needed a lower pressure and the feed stream evaporated much easily. The discharged side of the compressor satisfied the operating conditions of both pressure and temperature at the inlet of the reactor. And the reactor effluent stream was able to be used completely to preheat and vaporize the feed stream. All the heat in the process stream was re-circulated without using a fired heater. SHRT was applied to the naphtha HDS process of 18,000 barrel per stream day (BPSD) in the refinery and the mass and energy balance of the process was calculated using commercially available simulation software, Invensys PROII version 8.1. This process-simulation case study confirmed that despite there being no more energy saving potential in the conventional process that makes use of a fired heater, the advanced process with SHRT can reduce the energy consumption significantly by using the recuperated heat of the feed stream.     

Enzymatic digestion of corncobs pretreated with low strength of sulfuric acid for bioethanol production

In this study, the effect and the optimum pretreatment condition of corncobs using low strength of H₂SO₄ were investigated, in which H₂SO₄ was used to improve the enzymatic digestibility of corncobs for saccharification without degradation of sugars released. The optimum pretreatment condition was found to be the addition of 0.5% (vol./vol.) H₂SO₄ and autoclaving at 122 °C for 20 min. Under this condition, the structural integrity of corncob was altered to make cellulose microfibrils more accessible for cellulase enzymes, and the enzymatic digestion of corncobs could be significantly enhanced. A high yield of sugar, 80% (wt./wt.), could be obtained at a low enzyme dosage of 0.024 g enzymes/g cobs, when pretreated. As a result, the ethanol production was obviously improved by the pretreatment, i.e., the ethanol yield of 77% (wt./wt.) was obtained within 36 h in the SSF fermentation using Saccharomyces cerevisiae NBRC2114.     

Protective effects of galacturonic acid-rich vinegar brewed from Japanese pear in a dextran sodium sulfate-induced acute colitis model

The effects of pear vinegar (PV), which was specially brewed for enhanced galacturonic acid content, on the DSS-induced ulcerative colitis (UC) mouse model were evaluated. PV improved clinical symptoms, colon inflammation, and histological tissue injury in the DSS-induced acute UC mouse model. Moreover, PV suppressed inflammation due to acute UC by suppressing the myeloperoxidase (MPO)-mediated activation of inflammatory cells such as leukocytes and decreasing the serum concentration of IL-6. Our results demonstrated the protective action of PV in the DSS-induced acute UC mouse model. On the other hand, commercial apple vinegar did not show a protective effect in the DSS-induced acute UC mouse model. Our findings indicate that PV may act as a new functional food for inflammatory bowel disease patients.     

Bifurcation mechanism underlying echelon-mode formation

This paper presents a theory on the underlying mathematical mechanism of the echelon mode (a series of parallel short wrinkles that looks like a flight of stairs or wild geese arranged in formation) which has been observed ubiquitously with uniform materials, but which has long denied successful numerical simulations. It is shown by means of the group-theoretic bifurcation theory that the echelon mode formation can be explained as a recursive (secondary, tertiary, …) symmetry-breaking bifurcation if O(2) × O(2) is chosen as the underlying symmetry to model the local uniformity of materials. This implies, for example, that the use of periodic boundaries is essential to successfully realize the oblique stripe patterns and the subsequent echelon mode formation in numerical simulations. In fact, a recursive bifurcation analysis of a rectangular domain with periodic boundaries subject to uniform uniaxial compression yields various kinds of patterns, such as diamond, stripe and echelon modes, which are often observed for materials under shear.