An Interoperable Grid Information System for Integrated Resource Monitoring Based on Virtual Organizations

In many Grid infrastructures different kinds of information services are in use, which utilize different incompatible data structures and interfaces to encode and provide their data. Homogeneous monitoring of these infrastructures with the monitoring data being accessible everywhere independently of the middleware which provided it, is the basis for a consistent status reporting on the Grids’ resources and services. Thus, interoperability or interoperation between the different information services in a heterogeneous Grid infrastructure is required. Monitoring data must contain the identity of the affected Virtual Organization (VO) so that it can be related to the resources and services the VO has allocated to enable VO-specific information provision. This paper describes a distributed architecture for an interoperable information service, which combines data unification and categorization with policies for VO membership, VO resource management and data transformations. This service builds the basis for an integrated and interoperating monitoring of Grids, which provide their data to more than one VO and utilize heterogeneous information services.     

Acclimatization model of an aerobic bioreactor for the treatment of toxic wastewater

This work proposes a mathematical model for the acclimatization process of a bioreactor treating toxic wastewater. Experimental data was used to identify the changing kinetic parameters of the model as acclimatization progresses. It was found that only one key parameter, the specific biomass growth rate function, changed during the acclimatization process. Therefore, an acclimatization model was proposed to explain the changes of this parameter.     

AC electrokinetic pumping on symmetric electrode arrays

AC electro-osmotic (ACEO) pumping is experimentally demonstrated on a symmetric gold electrode array. Using asymmetric connection of electrodes to the applied AC voltage, spatial asymmetry along the array is created, which produces unidirectional flow of electrolyte. An aqueous solution of 100 μM KCl is selected as the pumping fluid. The liquid velocity obtained as a function of voltage and frequency is compared to that generated using travelling-wave electroosmosis (TWEO) with the same electrode array. The expected velocities from the linear electrokinetic models of ACEO and TWEO are computed numerically. The comparison shows that TWEO generates greater velocity amplitudes and the streamlines are smoother than those generated by ACEO.     

Optimum design of long-span suspension bridges considering aeroelastic and kinematic constraints

Cable supported bridges are wind prone structures. Therefore, their aerodynamic behaviour must be studied in depth in order to guarantee their safe performance. In the last decades important achievements have been reached in the study of bridges under wind-induced actions. On the other hand, non-conventional design techniques such as sensitivity analysis or optimum design have not been applied although they have proved their feasibility in the automobile or aeronautic industries. The aim of this research work is to demonstrate how non-conventional design techniques can help designers when dealing with long span bridges considering their aeroelastic behaviour. In that respect, the comprehensive analytical optimum design problem formulation is presented. In the application example the optimum design of the challenging Messina Strait Bridge is carried out. The chosen initial design has been the year 2002 design proposal. Up to a 33% deck material saving has been obtained after finishing the optimization process.     

Charting online OMICS resources: A navigational chart for clinical researchers

The life sciences have sprouted several popular and successful OMICS technologies that span all levels of biological information transfer. Ever since the start of the Human Genome Project, the then revolutionary idea to make all resulting data publicly available has been central to all of the efforts across OMICS technologies. As a result, a great variety of publicly available data repositories and resources is currently available to the research community. This widespread availability of data does come at the price of increased confusion on the part of the users, especially for those that see the OMICS technologies as tools to help unravel a larger biological or clinical question. We therefore provide a comprehensive overview of the available resources across OMICS fields, with a special emphasis on those databases that are relevant to the study of proteins. Additionally, we also describe various integrative systems that have been established, and highlight new developments in the field that can revolutionize the way in which live data integration is achieved over the internet.     

Proteomics identification of differentially expressed proteins in the muscle of dysferlin myopathy patients

The muscular dystrophies are a large and heterogeneous group of neuromuscular disorders that can be classified according to the mode of inheritance, the clinical phenotype and the molecular defect. To better understand the pathological mechanisms of dysferlin myopathy we compared the protein-expression pattern in the muscle biopsies of six patients with this disease with six patients with limb girdle muscular dystrophy 2A, five with facioscapulohumeral dystrophy and six normal control subjects. To investigate differences in the expression levels of skeletal muscle proteins we used 2-DE and MS. Western blot or immunohistochemistry confirmed relevant results. The study showed specific increase expression of proteins involved in fast-to-slow fiber type conversion (ankyrin repeat protein 2), type I predominance (phosphorylated forms of slow troponin T), sarcomere stabilization (actinin-associated LIM protein), protein ubiquitination (TRIM 72) and skeletal muscle differentiation (Rho-GDP-dissociation inhibitor ly-GDI) in dysferlin myopathy. As anticipated, we also found differential expression of proteins common to all the muscular dystrophies studied. This comparative proteomic analysis suggests that in dysferlin myopathy (i) the type I fiber predominance is an active process of fiber type conversion rather than a selective loss of type II fibers and (ii) the dysregulation of proteins involved in muscle differentiation further confirms the role of dysferlin in this process.     

Proteomic analysis in cerebrospinal fluid of patients with atypical nonketotic hyperglycinemia and pulmonary hypertension - A pilot study

A variant phenotype of nonketotic hyperglycinemia has been described by our group associated with pulmonary hypertension. The aim of this study is to investigate the cerebrospinal fluid proteomes to get an insight into this neurodegenerative process producing leukoencephalopathy with white matter spongiform degeneration. DIGE and MALDI-TOF-TOF analyses were performed to carry out the proteomic study of four patients against three normal controls and one additional control of a classical nonketotic hyperglycinemia. The differential proteomic analysis showed a displacement of some series of spots toward the acidic side. The shifted proteins showed a high degree of carbonylation and increased methionine sulfoxidation was found in cystatin C and in vitamin-D-binding protein. These findings in addition to the increase of serum malondialdehyde concentration provide evidence of an oxidative stress in the patients under study, which is probably systemic rather than mainly confined to the CNS. The similarities of our findings with those found in other neurodegenerative diseases suggest that oxidative damage is commonly involved in these pathologies. DIGE technology improves the 2-D PAGE differential analysis and it is suitable in proteomic studies with a small number of cases.     

Inductively coupled probe for the measurement of partial discharges

Partial discharges are a transient phenomena whose measurement is remarkably important for electrical equipment diagnosis and maintenance. These discharges appear in the measurement circuit as very narrow current pulses of some nanoseconds. Therefore, discharge pulse detection is a particularly difficult problem, especially because they are superposed on the high voltage waveforms that cause them. In this paper, we present an inductively coupled probe able to measure this physical phenomenon by means of a very simple and inexpensive device that can be installed in the equipment under test. After modeling the probe, its response will be compared to that of commercial devices using both calibrated discharges and partial discharges occurring at real power.     

On the interpretation of the forbidden spots observed in the electron diffraction patterns of flat Au triangular nanoparticles

In many cases nanostructures present forbidden spots in their electron diffraction patterns when they are observed by transmission electron microscopy (TEM). To interpret their TEM and high resolution transmission electron microscopy (HRTEM) images properly, an understanding of the origin of these spots is necessary. In this work we comment on the origin of the forbidden spots observed in the [111] and [112] electron diffraction patterns of flat gold triangular nanoparticles. The forbidden spots were successfully indexed as corresponding to the first laue Zone (FOLZ) and the HRTEM images presented a contrast produced by the interference of the zero-order Laue zone (ZOLZ) and FOLZ spots. We discuss the use of the forbidden spots in the study of the structure of nanoparticles and show that they are related to the shape and incompleteness of layers in the very thin particles.     

Coulomb interactions in Ga LMIS

For low emission currents from around 1 microA Ga liquid-metal ion sources (LMIS) produce fine optically bright ion beams that are strongly limited by the Coulomb particle-particle interactions. We present computations of the energy spread, the beam virtual crossover size, and beam brightness based on direct numerical integration of the equation of motion in a numerically calculated field for a number of dimensions of the emission tip. The Coulomb particle-particle interactions are included into the calculation of ion beam evolution. A comparison with experimental data allows to estimate the tip size.