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61.
62.
Oliver Schmutzler Sebastian Graf Nils Behm Wael Y. Mansour Florian Blumendorf Theresa Staufer Christian Krnig Dina Salah Yanan Kang Jan N. Peters Yang Liu Neus Feliu Wolfgang J. Parak Anja Burkhardt Elisabetta Gargioni Sabrina Gennis Sharah Chandralingam Finn Heg Wolfgang Maison Kai Rothkamm Florian Schulz Florian Grüner 《International journal of molecular sciences》2021,22(7)
Quantitative cellular in vitro nanoparticle uptake measurements are possible with a large number of different techniques, however, all have their respective restrictions. Here, we demonstrate the application of synchrotron-based X-ray fluorescence imaging (XFI) on prostate tumor cells, which have internalized differently functionalized gold nanoparticles. Total nanoparticle uptake on the order of a few hundred picograms could be conveniently observed with microsamples consisting of only a few hundreds of cells. A comparison with mass spectroscopy quantification is provided, experimental results are both supported and sensitivity limits of this XFI approach extrapolated by Monte-Carlo simulations, yielding a minimum detectable nanoparticle mass of just 5 pg. This study demonstrates the high sensitivity level of XFI, allowing non-destructive uptake measurements with very small microsamples within just seconds of irradiation time. 相似文献
63.
Advances in Modeling and Simulation of Grinding Processes 总被引:6,自引:1,他引:6
E. Brinksmeier E. Govekar H.-W. Hoffmeister J. Peters D.J. Stephenson K. Weinert 《CIRP Annals》2006,55(2):667-696
In the last decade the relevance of modeling and simulation of grinding processes has significantly risen which is caused by industrial needs and is indicated by the number of publications and research activities in this area. This keynote paper results from a collaborative work within the STC G and gives an overview of the current state of the art in modeling and simulation of grinding processes: Physical process models (analytical and numerical models) and empirical process models (regression analysis, artificial neural net models) as well as heuristic process models (rule based models) are taken into account, and outlined with respect to their achievements in this paper. The models are characterized by the process parameters such as grinding force, grinding temperature, etc. as well as work results including surface topography and surface integrity. Furthermore, the capabilities and the limitations of the presented model types and simulation approaches will be exemplified. 相似文献
64.
A mathematical model and its numerical solution is presented to describe adiabatic adsorption—desorption processes in a fixed bed when the mass and heat transfer can be described by external film transfer coefficients only, which is especially interesting in the case of non-uniform, irregular particles. A new method to measure such rates is presented, based on continuous weighing of a through-circulated bed. The measured rates can be checked against the difference in humidity and temperature between the outlet and inlet air in order to minimize errors.The comparison between experimental results and calculations showed that the adsorption process in a bed of molecular sieve pellets can not be described with a constant mass transfer coefficient; the desorption process is better described although not entirely satisfactorily. In the case of a honeycomb molecular sieve, a constant mass transfer coefficient described both the adsorption and desorption processes satisfactorily. 相似文献
65.
Condensation of 4–chloro–l, 8–naphthalic anhydride with 2–nitrophenol afforded 4–(2–nitrophenoxy)–l, 8–naphthalic anhydride, which on reduction followed by Pschorr intramolecular cyclisation gave benzo[k, l]xanthene–3, 4–dicarboxylic acid anhydride. The identity of the product was confirmed by its alternative synthesis from 5–amino–4–phenoxy–l, 8–naphthalic anhydride. Benzo[k, l]xanthene–3, 4–dicarbox–ylic acid anhydride condensed with alkylamines or arylamines and with o–phenylenediamines to yield the corresponding imides and imidazole derivatives, which coloured polyester in fluorescent greenish–yellow to orange hues, respectively, of good fastness to light and sublimation. The colour of the dyes is discussed with respect to analogous ring–closed sulphur– containing heterocycles. 相似文献
66.
Mickaël Causse Roland Alonso François Vachon Robert Parise Jean-Pierre Orliaguet Sébastien Tremblay 《Ergonomics》2014,57(11):1616-1627
This study aims to determine whether indirect touch device can be used to interact with graphical objects displayed on another screen in an air traffic control (ATC) context. The introduction of such a device likely requires an adaptation of the sensory-motor system. The operator has to simultaneously perform movements on the horizontal plane while assessing them on the vertical plane. Thirty-six right-handed participants performed movement training with either constant or variable practice and with or without visual feedback of the displacement of their actions. Participants then performed a test phase without visual feedback. Performance improved in both practice conditions, but accuracy was higher with visual feedback. During the test phase, movement time was longer for those who had practiced with feedback, suggesting an element of dependency. However, this ‘cost’ of feedback did not extend to movement accuracy. Finally, participants who had received variable training performed better in the test phase, but accuracy was still unsatisfactory. We conclude that continuous visual feedback on the stylus position is necessary if tablets are to be introduced in ATC. 相似文献
67.
This paper addresses the problem of defining a simple End-Effector design for a robotic arm that is able to grasp a given set of planar objects. The OCOG (Objects COmmon Grasp search) algorithm proposed in this paper searches for a common grasp over the set of objects mapping all possible grasps for each object that satisfy force closure and quality criteria by taking into account the external wrenches (forces and torque) applied to the object. The mapped grasps are represented by feature vectors in a high-dimensional space. This feature vector describes the design of the gripper. A database is generated for all possible grasps for each object in the feature vector space. A search algorithm is then used for intersecting all possible grasps over all parts and finding a common grasp suitable for all objects. The search algorithm utilizes the kd-tree index structure for representing the database of the sets of feature vectors. The kd-tree structure enables an efficient and low cost nearest-neighbor search for common vectors between the sets. Each common vector found (feature vector) is the grasp configuration for a group of objects, which implies the future end-effector design. The final step classifies the grasps found to subsets of the objects, according to the common vectors found. Simulations and experiments are presented for four objects to validate the feasibility of the proposed algorithm. The algorithm will be useful for standardization of end-effector design and reducing its engineering time. 相似文献
68.
The synthesis of a series of dyes derived from reaction of 2-methyl-4-(N-ethyl-N-hydroxyethyl)amino, lidicyano-styrene with acid chlorides, chloroformates, isocyanates and isothiocyanates is described. Data relevant to dye characterization by electron-impact induced fragmentation is reported. Dyeing and fastness properties of the dyes on cellulose secondary acetate, cellulose triacetate, nylon 6.6 and polyester fibres are discussed in terms of dye structure. Comparison is made between some related dyes derived from 2-methyl-4-(N-ethyl-N-β-aminoethyljamino-β,βdicyanostyrene. 相似文献
69.
70.
Köhler K Lellouch AC Vollmer S Stoevesandt O Hoff A Peters L Rogl H Malissen B Brock R 《Chembiochem : a European journal of chemical biology》2005,6(1):152-161
Cellular signal transduction proceeds through a complex network of molecular interactions and enzymatic activities. The timing of these molecular events is critical for the propagation of a signal and the generation of a specific cellular response. To define the timing of signalling events, we introduce the combination of high-resolution confocal microscopy with the application of small-molecule inhibitors at various stages of signal transduction in T cells. Inhibitors of Src-family tyrosine kinases and actin dynamics were employed to dissect the role of the lymphocyte-specific tyrosine kinase Lck in the formation and maintenance of T cell receptor/CD3-dependent contacts. Anti-CD3epsilon-coated coverslips served as a highly defined stimulus. The kinetics of the recruitment of the yellow fluorescent protein-tagged signalling protein ZAP-70 were detected by high-resolution confocal microscopy. The analysis revealed that at 5 min after receptor engagement, Lck activity was required for maintenance of contacts. In contrast, after 20 min of receptor engagement, the contacts were Lck-independent. The relevance of the timing of inhibitor application provides a pharmacological concept for the maturation of T cell-substrate contacts. 相似文献