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排序方式: 共有381条查询结果,搜索用时 31 毫秒
71.
Dr. Yan Shan Ang Dr. Toshikazu Bando Prof. Hiroshi Sugiyama Dr. Lin-Yue Lanry Yung 《Chembiochem : a European journal of chemical biology》2020,21(20):2912-2915
We used N-methylpyrrole (Py)-N-methylimidazole-(Im) polyamide as an exogenous agent to modulate the formation of DNA assemblies at specific double-stranded sequences. The concept was demonstrated on the hybridization chain reaction that forms linear DNA. Through a series of melting curve analyses, we demonstrated that the binding of Py−Im polyamide positively influenced both the HCR initiation and elongation steps. In particular, Py−Im polyamide was found to drastically stabilize the DNA duplex such that its thermal stability approached that of an equivalent hairpin structure. Also, the polyamide served as an anchor between hairpin pairs in the HCR assembly, thus improving the originally weak interstrand stability. We hope that these proof-of-concept results can inspire future use of Py−Im polyamide as a molecular tool to modulate the formation of DNA assemblies. 相似文献
72.
73.
Hiroya Maeda Takehiro Kashiyama Yoshihide Sekimoto Toshikazu Seto Hiroshi Omata 《Computer-Aided Civil and Infrastructure Engineering》2021,36(1):47-60
Machine learning can produce promising results when sufficient training data are available; however, infrastructure inspections typically do not provide sufficient training data for road damage. Given the differences in the environment, the type of road damage and the degree of its progress can vary from structure to structure. The use of generative models, such as a generative adversarial network (GAN) or a variational autoencoder, makes it possible to generate a pseudoimage that cannot be distinguished from a real one. Combining a progressive growing GAN along with Poisson blending artificially generates road damage images that can be used as new training data to improve the accuracy of road damage detection. The addition of a synthesized road damage image to the training data improves the F‐measure by 5% and 2% when the number of original images is small and relatively large, respectively. All of the results and the new Road Damage Dataset 2019 are publicly available ( https://github.com/sekilab/RoadDamageDetector ). 相似文献
74.
75.
Tanzo Nitta Yasuyuki Shirai Toshikazu Kawauchi Takao Okada Yoshihiro Ogawa 《Electrical Engineering in Japan》1995,115(6):62-70
Experimental studies using20-kVA superconducting generators (SCG) have been performed in an attempt to understand some of the characteristics of SCG in power systems. It is necessary to investigate the characteristics for the parallel operations of both an SCG and a conventional generator (CG) when an SCG is installed in a present power station. Some of the results of the authors' studies on parallel running of the 20-kVA SCG and the 20-kVA CG are described in this paper. In the experimental system, the SCG is connected to a regional power system (infinite bus) through reactors (artificial transmission lines) and the CG is connected to the terminal of the SCG. Using experiments and computer simulations, the transient characteristics at three-phase short-circuit faults are investigated: 1) when the CG is not disconnected; and 2) when the CG is disconnected after reclosing the line with the fault cleared. Two kinds of stable limit curves are obtained for output power at three-phase short-circuit fault for the aforementioned two cases. 相似文献
76.
Leak flow rate and jet impingement load are experimentally evaluated using very narrow and short artificial slits which simulate a through-wall fatigue crack. The following items are clarified in the experiments under BWR fluid conditions: (1) the leak flow rate from the rectangular slit is experimentally clarified and it agrees well with the analytical result when the estimated thermodynamic nonequilibrium parameter N in the present experiment is introduced; (2) the orifice effects are experimentally evaluated concerning the through-wall fatigue crack, in which the discharge coefficients CD between 0.75 and 0.25 are obtained as a function of the exit to the stagnation area ratio Ae/Ao; (3) when very narrow and short slits are used in the jet impingement experiments, the maximum pressure on the target is higher than that of a large diameter pipe and it increases with the decrease of the flow path length to hydraulic diameter ratio L/D of the slit because of the thermodynamic nonequilibrium effect. 相似文献
77.
Elastic properties of real elastomers were examined by using partial derivatives of the strain energy density function (W) with respect to the invariant (Ii, i=1,2,3) of deformation tensor. The values of the derivatives at zero strain limit for styrene-butadiene rubber (SBR), nitrile-butadiene rubber (NBR) and butadiene rubber (BR) were compared with those derived from the theory shown in the previous paper, where compressibility of the materials was taken into account. The asymptotic behavior of the derivatives for isoprene rubber (IR) and segmented polyurethaneurea (SPU) was also compared with those of the three types of rubbers. It was shown that the theoretical predictions for the limiting value agreed fairly well with the experimental values of the elastomers. 相似文献
78.
79.
The permeabilities of He, H2, N2, O2, CO2, CH4, C2H6, C3H8, and n-C4H10 in poly[1-phenyl-2-[p-(trimethylsilyl)phenyl]acetylene] (PTMSDPA) and poly[diphenylacetylene] (PDPA) are presented and compared to those of poly(1-trimethylsilyl-1-propyne) (PTMSP), poly(1-phenyl-1-propyne) (PPP), and polysulfone. Like PTMSP, PTMSDPA, a disubstituted glassy acetylene-based polymer, exhibits higher permeabilities to organic vapors than to permanent gases due to its rigid polyacetylene backbone and bulky side groups, which provide a relatively high fractional free volume (FFV) value of 0.26. Desilylation was performed on PTMSDPA. The resulting material, PDPA, is totally insoluble in common organic solvents, so it has much higher chemical resistance than PTMSDPA. Additionally, due to its insolubility in polymerization solvents, desilylation provides the only known route to high molar mass PDPA. The FFV of the resulting membrane (PDPA) is reduced by approximately 12% relative to that of PTMSDPA. This leads to a decrease in gas permeability values and selectivity of organic vapors relative to nitrogen. For example, the oxygen permeability is reduced from 1200 to 500 Barrers upon desilylation. The pure gas selectivities decrease from 9 to 3 for n-C4H10/N2 and from 26 to 9 for C3H8/N2. 相似文献
80.
Masaki Himejima Kenneth R. Hobson Toshikazu Otsuka David L. Wood Isao Kubo 《Journal of chemical ecology》1992,18(10):1809-1818
The oleoresin of the ponderosa pine,Pinus ponderosa (Pinaceae) exhibited broad antimicrobial activity. In order to identify the active compounds, the oleoresin was steam distilled
to give a distillate and residue. The distillate contained mainly monoterpenes and some sesquiterpenes, while the residue
consisted chiefly of four structurally related diterpene acids. An antimicrobial assay with the pure compounds indicated that
the monoterpenes were active primarily against fungi, but there was also some activity against gram-positive bacteria. The
diterpene acids, in contrast, only exhibited activity against gram-positive bacteria. Although not all of the identified sesquiterpenes
could be tested, longifolene showed activity only against gram-positive bacteria. Therefore, it appears that the oleoresin
ofP. ponderosa functions as a biochemical defense against microbial invasion. 相似文献