Evaluating different fixation protocols for spectral cytopathology, part 1

Spectral cytopathology (SCP) is a novel approach for disease diagnosis that utilizes infrared spectroscopy to interrogate the biochemical components of cellular samples and multivariate statistical methods, such as principal component analysis, to analyze and diagnose spectra. SCP has taken vast strides in its application for disease diagnosis over the past decade; however, fixation-induced changes and sample handling methods are still not systematically understood. Conversely, fixation and staining methods in conventional cytopathology, typically involving protocols to maintain the morphology of cells, have been documented and widely accepted for nearly a century. For SCP, fixation procedures must preserve the biochemical composition of samples so that spectral changes significant to disease diagnosis are not masked. We report efforts to study the effects of fixation protocols commonly used in traditional cytopathology and SCP, including fixed and unfixed methods applied to exfoliated oral (buccal) mucosa cells. Data suggest that the length of time in fixative and duration of sample storage via desiccation contribute to minor spectral changes where spectra are nearly superimposable. These findings illustrate that changes influenced by fixation are negligible in comparison to changes induced by disease.     

Ruthenium complex containing block copolymer for the enhancement of carbon nanotube photoconductivity

We report the synthesis of a multifunctional block copolymer incorporated with pyrene and ruthenium terpyridyl thiocyanato complex moieties by reversible addition-fragmentation chain transfer polymerization. The pyrene block in the copolymer facilitates the dispersion of multiwalled carbon nanotubes in DMF solution because of the strong π-π interaction between the pyrene moieties and nanotube surface. On the other hand, the ruthenium complexes greatly enhance the photosensitivity of the functionalized nanotubes in the visible region. The photocurrent responses of the nanotubes at different wavelength measured by conductive AFM spectrum strongly agree with the absorption spectrum of the ruthenium complex. The results demonstrate a new and versatile approach in enhancing and fine-tuning the photosensitivity or other opto-electronic properties of carbon nanotubes by multifunctional block copolymers.     

A localized algorithm for bi-connectivity of connected mobile robots

Teams of multiple mobile robots may communicate with each-other using a wireless ad-hoc network. Fault-tolerance in communication can be achieved by making the communication network bi-connected. We present the first localized protocol for constructing a fault-tolerant bi-connected robotic network topology from a connected network, in such a way that the total movement of robots is minimized. The proposed distributed algorithm uses p-hop neighbor information to identify critical head robots that can direct two neighbors to move toward each other and bi-connect their neighborhood. Simulation results show that the total distance of movement of robots decreases significantly (e.g. about 2.5 times for networks with density 10) with our localized algorithm when compared to the existing globalized one. Proposed localized algorithm does not guarantee bi-connectivity, may partition the network, and may even stop at connected but not bi-connected stage. However, our algorithm achieved 100% success on all networks with average degrees ≥10, and over 70% success on sparse networks with average degrees ≥5.     

Optimal log-polar image sampling

In order to achieve better image compression simultaneously maintaining the high signal quality, the image sampling has become very important. Also, since the human eye sensitivity has circularly symmetric distribution, in recent years it is usual to apply log-polar image sampling. In this paper, we perform optimization of log-polar image sampling and show the significant improvement in comparison with the product log-polar sampling. Namely, for equal numbers of sensors optimal model gives higher signal-to-noise ratio (SNR) up to 2.5 dB, i.e., it is possible to decrease the number of required sensors by 45% for the same SNR. Furthermore, research shows that in optimal log-polar image sampling, the middle region of image, which is not sampled, can be made to be smaller than in the case of product sampling.     

Distributed Dijkstra sparse placement routing algorithm for translucent optical networks

In this paper we study translucent optical networks as an alternative to fully transparent and fully opaque optical networks. In the former networks, a technique called sparse placement is used to overcome the lightpath blocking caused by the signal quality degradation, using much less regenerators, which must strategically be placed, in contrast to a fully opaque network. In this paper we propose a sparse placement algorithm based on two requirements. The first one is signal regeneration necessary to re-amplify, reshape, and retime the optical signals after some predefined transparent distance in order to successfully receive the signals at the destination node. The other is load balance of the traffic in the network aimed at efficient usage of the network capacity resources. We apply a distributed Dijkstra routing algorithm which dynamically changes weights of links during the process of locating regeneration capable nodes. We compare the performance of the proposed algorithm with commonly used sparse placement algorithms through simulation experiments. The benefits are such that load balancing of the network traffic is fully utilized, and with technological development it will be sufficient to equip up to 30% of nodes in the network with electronic regenerations in order to have the same performance as in an opaque network.

Methylglyoxal in food and living organisms

Methylglyoxal (MG) is a highly reactive alpha-oxoaldehyde formed endogenously in numerous enzymatic and nonenzymatic reactions. It modifies arginine and lysine residues in proteins forming advanced glycation end-products such as N(delta)-(5-methyl-4-imidazolon-2-yl)-L-ornithine (MG-H1), 2-amino-5-(2-amino-5-hydro-5-methyl-4-imidazolon-1-yl)pentanoic acid (MG-H2), 2-amino-5-(2-amino-4-hydro-4-methyl-5-imidazolon-1-yl)pentanoic acid (MG-H3), argpyrimidine, N(delta)-(4-carboxy-4,6-dimethyl-5,6-dihydroxy-1,4,5,6-tetrahydropyrimidine-2-yl)-L-ornithine (THP), N(epsilon)-(1-carboxyethyl)lysine (CEL), MG-derived lysine dimer (MOLD), and 2-ammonio-6-({2-[4-ammonio-5-oxido-5-oxopently)amino]-4-methyl-4,5-dihydro-1H-imidazol-5-ylidene}amino)hexanoate (MODIC), which have been identified in vivo and are associated with complications of diabetes and some neurodegenerative diseases. In foodstuffs and beverages, MG is formed during processing, cooking, and prolonged storage. Fasting and metabolic disorders and/or defects in MG detoxification processes cause accumulation of this reactive dicarbonyl in vivo. In addition, the intake of low doses of MG over a prolonged period of time can cause degenerative changes in different tissues, and can also exert anticancer activity. MG in biological samples can be quantified by HPLC or GC methods with preliminary derivatization into more stable chromophores and/or fluorophores, or derivatives suitable for determination by MS by use of diamino derivatives of benzene and naphthalene, 6-hydroxy-2,4,5-triaminopyrimidine, cysteamine, and o-(2,3,4,5,6-pentafluorobenzyl) hydroxylamine. The methods include three basic steps: deproteinization, incubation with derivatization agent, and chromatographic analysis with or without preliminary extraction of the formed products.     

Multifractal analysis of human retinal vessels

In this paper, it is shown that vascular structures of the human retina represent geometrical multifractals, characterized by a hierarchy of exponents rather then a single fractal dimension. A number of retinal images from the STARE database are analyzed, corresponding to both normal and pathological states of the retina. In all studied cases, a clearly multifractal behavior is observed, where capacity dimension is always found to be larger then the information dimension, which is in turn always larger then the correlation dimension, all the three being significantly lower then the diffusion limited aggregation (DLA) fractal dimension. We also observe a tendency of images corresponding to the pathological states of the retina to have lower generalized dimensions and a shifted spectrum range, in comparison with the normal cases.     

Insulation co-ordination for continuous operating voltages and temporary overvoltages

This paper presents an approach to the insulation co-ordination of electric-power installations in the scope of the continuous operating voltages and temporary overvoltages; this approach opens the possibility to calculate the necessary required withstand voltages of unit insulations for short-duration (1 min) power-frequency test voltage, with respect to continuous operating voltages and power-frequency temporary overvoltages and the necessary reliability of the operation of high-voltage power system installations.     

α- and γ-Hexachlorocyclohexane measurements in the brine fraction of sea ice in the Canadian High Arctic using a sump-hole technique

We used holes augered partially into first-year sea ice (sumps) to determine α- and γ-HCH concentrations in sea-ice brine. The overwintering of the CCGS Amundsen in the Canadian western Arctic, as part of the Circumpolar Flaw Lead (CFL) System Study, provided the circumstances to allow brine to accumulate in sumps sufficiently to test the methodology. We show, for the first time, that as much as 50% of total HCHs in seawater can become entrapped within the ice crystal matrix. On average, in the winter first-year sea ice HCH brine concentrations reached 4.013 ± 0.307 ng/L and 0.423 ± 0.013 ng/L for the α- and γ-isomer, respectively. In the spring, HCHs decreased gradually with time, with increasing brine volume fraction and decreasing brine salinity. These decreasing concentrations could be accounted for by both the dilution with the ice crystal matrix and under-ice seawater. We propose that the former process plays a more significant role considering brine volume fractions calculated in this study were below 20%. Levels of HCHs in the brine exceed under-ice water concentrations by approximately a factor of 3, a circumstance suggesting that the brine ecosystem has been, and continues to be, the most exposed to HCHs.     

Sol–gel processed thin-layer ruthenium oxide/carbon black supercapacitors: A revelation of the energy storage issues

Hydrous ruthenium oxide/carbon black nanocomposites were prepared by impregnation of the carbon blacks by differently aged inorganic RuO₂ sols, i.e. of different particle size. Commercial Black Pearls 2000^® (BP) and Vulcan^® XC-72 R (XC) carbon blacks were used. Capacitive properties of BP/RuO₂ and XC/RuO₂ composites were investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) in H₂SO₄ solution. Capacitance values and capacitance distribution through the composite porous layer were found different if high- (BP) and low- (XC) surface-area carbons are used as supports. The aging time (particle size) of Ru oxide sol as well as the concentration of the oxide solid phase in the impregnating medium influenced the capacitive performance of prepared composites. While the capacitance of BP-supported oxide decreases with the aging time, the capacitive ability of XC-supported oxide is promoted with increasing oxide particle size. The increase in concentration of the oxide solid phase in the impregnating medium caused an improvement of charging/discharging characteristics due to pronounced pseudocapacitance contribution of the increasing amount of inserted oxide. The effects of these variables in the impregnation process on the energy storage capabilities of prepared nanocomposites are envisaged as a result of intrinsic way of population of the pores of carbon material by hydrous Ru oxide particle.