We have identified and cloned the cDNAs encoding two odorant-binding proteins (OBPs) from the American palm weevil (APW) Rhynchophorus palmarum (Coleoptera, Curculionidae). Degenerate primers were designed from the N-terminal sequences and were used in polymerase chain reaction (PCR) in order to obtain full-length sequences in both males and females. In both sexes, two different cDNAs were obtained, encoding 123 and 115 amino acid-deduced sequences. Each sequence showed few amino acid differences between the sexes. The proteins were named RpalOBP2 and RpalOBP4 for male, RpalOBP2' and RpalOBP4' for female, with the types 2 and 4 presenting only 34% identities. These proteins shared high identity with previously described coleopteran OBPs. In native gels, RpalOBP2 clearly separated into two bands and RpalOBP4 into three bands, suggesting the presence of several conformational isomers. Thus, OBP diversity in this species may rely on both the presence of OBPs from different classes and the occurrence of isoforms for each OBP. 相似文献
Results from 2 experimental studies suggest that self-protection and mate-search goals lead to the perception of functionally relevant emotional expressions in goal-relevant social targets. Activating a self-protection goal led participants to perceive greater anger in Black male faces (Study 1) and Arab faces (Study 2), both out-groups heuristically associated with physical threat. In Study 2, participants' level of implicit Arab-threat associations moderated this bias. Activating a mate-search goal led male, but not female, participants to perceive more sexual arousal in attractive opposite-sex targets (Study 1). Activating these goals did not influence perceptions of goal-irrelevant targets. Additionally, participants with chronic self-protective and mate-search goals exhibited similar biases. Findings are consistent with a functionalist, motivation-based account of interpersonal perception. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
The advantage of thermoplastic molding as a convenient method for fabricating large quantities of microstructures is restricted
by the possibilities and the necessary efforts for structuring the required molding tools. In order to increase the complexity
of mold inserts without significantly increasing the fabrication expenditure a new process combining LIGA techniques and precision
mechanics had been suggested by Research Center Karlsruhe. Recent work on the optimization of this process made it possible
to manufacture multi-leveled mold inserts with which different three-dimensional microcomponents have successfully been molded.
The two- and three-level structures feature among other details integrated alignment aids which worked very well during the
assembly of the valve system the components were designed for.
This paper deals with the process optimization, the manufacturing of the mold inserts, the fabrication of the three-dimensional
microstructures by hot embossing in PMMA as well as in polymers with high thermal resistance and the application in a microvalve
system.
Received: 25 August 1997/Accepted: 22 September 1997 相似文献
The emissions of volatile organic compounds (VOCs) from building materials may significantly contribute to indoor air pollution, and VOCs have been associated with odor annoyance and adverse health effects. Wood materials together with coatings are commonly used indoors for furniture and large surfaces such as walls, floors, and ceilings. This leads to high surface-to-volume ratios, and therefore, these materials may participate remarkably to the VOC levels of indoor environment. We studied emissions of VOCs and carbonyl compounds from pinewood (Pinus sylvestris) boards of 10% and 16% moisture contents (MC) with three paints using small-scale test chambers (27 L). The emissions from uncoated pinewood and paints (on a glass substrate) were tested as references. The 28-day experiment showed that the VOC emissions from uncoated pinewood were lower from sample with 16% MC. Painted pinewood samples showed lower emissions compared to paints on glass substrate. Additionally, paints on 16% MC pinewood exhibited lower emissions than on drier 10% MC wood. The emissions from painted pinewood samples were dominated by paint-based compounds, but the share of wood-based compounds increased over time. However, we noticed differences between the paints, and wood-based emissions were clearly higher with the most permeable paint. 相似文献
This paper proposes new procedures for scheduling a flow-line manufacturing cell and compares the results obtained by using these new procedures to results obtained using procedures developed by Wemmerlov and Vakharia, Skorin-Kapov and Vakharia, and Sridhar and Rajendran. The procedures are evaluated on the basis of the makespans generated by solutions for test problems. The results show that the new procedures can efficiently generate good makespans and that results obtained by using the tabu search procedure developed by Skorin-Kapov and Vakharia can be improved if a starting set of job and family sequences is generated by one of the new procedures. 相似文献
Vibration analysis of square and circular piezoelectric micro ultrasonic transducers (pMUTs)
in the 100 kHz range as a function of experimental tools are reported. Analytical and 3D finite element method analysis using Comsol software has been performed to model static, modal and vibration behavior of these membranes. Comparison with standard impedancemeter measurement is shown to assess the performance of Laser Doppler Vibrometry system.
Mechanical and electrical characterization and comparison with a model results are presented and discussed. The measured resonances frequencies of membrane can be weak and superimposed on important parasitic signal,
which may mask the desired mechanical resonance signal. Our results revealed the real roles of the simulations and the combination of the experimental tools to get measurement accuracy.
Subsequently, this piezoelectric micro-transducer was successfully tested as a sounder in air. These investigations offer guidance for the pMUTs design and associated electronic circuit but might at the same time be instructive and beneficial to further sensor applications.