The impact of roasting on the ochratoxin A content of coffee

Roasting coffee led to a drop in the ochratoxin A (OTA) concentration, as measured by the reference method, especially for dark type roasts. The way the beverage was prepared also affected the OTA content, which could paradoxically be higher than that of the initial roasted coffee. Assays on the thermal stability of pure OTA showed that it ought to be found in larger quantities in roasted coffee. This suggested that OTA was masked by reactions with the substrate during roasting. The absence of OTA in green coffee is therefore the best guarantee of safety.     

A review on queueing network models with finite capacity queues for software architectures performance prediction

A review is carried out on how queueing network models with blocking have been applied so far into the performance evaluation and prediction of Software Architectures (SA). Queueing network models with finite capacity queues and blocking have recently been introduced and applied as more realistic models of systems with finite capacity resources and population constraints. Queueing network models have been often adopted as models for the evaluation of software performance. Starting from our own experience, we observe the need of a more accurate definition of the performance models of SA to capture some features of the communication systems. We consider queueing networks with finite capacity and blocking after service (BAS) to represent some synchronization constraints that cannot be easily modeled with queueing network models with infinite capacity queues. We investigate the use of queueing networks with blocking as performance models of SA with concurrent components and synchronous communication. Queueing theoretic analysis is used to solve the queueing network model and study the synchronous communication and performance of concurrent software components. Our experience is supported by other approaches that also propose the use of queueing networks with blocking. Directions for future research work in the field are included.     

Cooling of cylindrical vertical tanks submitted to natural internal convection

A numerical and an experimental analysis of velocity and temperature fields inside a storage tank submitted to natural convection is presented. The analysis was performed in two stages. In the first stage, the temperature profile along the vertical axis of the storage tank was obtained experimentally and numerically, for cooling time ranging from 45 to 60 h. The numerical analysis was carried out using a transient bi-dimensional model in cylindrical co-ordinates. In the second stage, after the numerical code validation, 40 cases of cooling with four aspect ratios, five insulation thicknesses, and two different volumes were simulated. In all simulations, thermal losses for the environment in all tank walls (side, top and bottom) were considered. Two correlations for the Nusselt number, encompassing all the forty cases, were obtained with these results.     

Stability studies on methyl and ethyl fatty acid esters of sunflowerseed oil

Fatty acid esters, high in linoleic acid, were prepared and stored for long-term engine tests. Storage tests with these esters were undertaken to obtain more information on optimal storage requirements and general stability characteristics. Samples were kept at three temperature levels (20 C, 30 C and fluctuating around 50 C) for a 90-day period and were removed at regular intervals for chemical and physical analysis. The influence of air, temperature, light, TBHQ and contact with mild steel was evaluated by comparing the free fatty acid, peroxide, anisidine, ultraviolet absorption, viscosity and induction periods. A statistical model was used to evaluate the data and to reduce the large number of data points to comparable curves. Storage of esters in contact with air, especially at a temperature above 30 C, resulted in significant increases in peroxide, ultraviolet absorption, free fatty acid, viscosity and anisidine values. Exclusion of air retarded oxidation at all temperature levels. A direct relationship between viscosity increases and oxidation parameters was evident. Exposure to light caused a small increase in the oxidation parameters of esters stored at the highest temperature level. Addition of TBHQ prevented oxidation of samples stored under moderate conditions. Under unfavorable storage conditions the anti-oxidant was no longer effective. Mild steel had very little effect on the oxidation parameters. Only the anisidine values of samples stored at the highest temperature level were slightly increased. Methyl esters performed slightly better than ethyl esters during the storage test. The following practical guidelines for storage of fatty acid ester fuels are: (i) airtight containers should be used; (ii) the storage temperature should be <30 C; (iii) mild steel (rust free) containers may be used, and (iv) TBHQ has a beneficial effect on oxidation stability.     

Optimised electromagnetic 3d field solver for frequencies below the first resonance

An optimised low-frequency solution algorithm is proposed that reduces the electromagnetic wave equation to a small number of scalar potential problems. The latter can be solved using black-box algebraic multigrid solvers. Within the framework of the finite integration technique, the reduction of the wave equation is accomplished by tree/cotree decompositions of primary and dual grids, which allow for highly efficient solutions of Ampere's and Faraday's equations. The example of a ferrite-loaded accelerator component demonstrates that the scheme is much more efficient than the direct approach using Krylov subspace solvers     

Extension of a combined analytical/numerical initial value problem solver for turbulent mixing with combustion

Here, we describe the development of a reacting flow multi-species/combustion methodology, implemented as an extension to the differential reduced ejector analysis (DREA) computer program [Mathematical and computer modeling, vol. 31, 2000, p. 21; Appl. Math. Model. 25 (2001) 427; Comput. Math. Appl. 43(10–11); NASA Contractor Report, 1998]. Use of the single fluid IVP solver framework that was developed for the original DREA model has been directly coupled into the combustion formulation. With these modifications, the analysis has an elementary single step reaction Fuel+Oxidizer→Product combustion capability. Though approximate in nature, the simplicity and efficiency of the DREA formulation make it suitable for its original niche, namely design and preliminary design environments where more complex and expensive models may be inappropriate.     

A result on the hyperstability of a class of hybrid dynamic systems

This paper presents a hyperstability theorem for a class of hybrid dynamic systems composed of coupled differential and difference equations subject to (possibly) time-varying nonlinearities satisfying a Popov-type inequality. The nonlinear controller generates the plant input at all times from its sampled values by defining an extended discrete system. The hyperstability results are obtained from this discrete system of special type whose state consists of the sampled continuous substate and the digital substate of the given hybrid system. Some corollaries and related physical interpretations are also given     

Acetohydroxy acid synthase and threonine deaminase activities, and the biosynthesis of isoleucine-leucine-valine in Streptococcus bovis

Acetohydroxy acid synthase (AHAS) and threonine deaminase (TD) activities were found in Streptococcus bovis and shown to be involved in the biosynthesis of the branched chain amino acids isoleucine, leucine and valine. Apparent lack of repression of AHAS synthesis by the end-products and reduced sensitivity of S. bovis growth to analogues of the branched chain amino acids suggested that secretion of isoleucine, leucine and valine in the growth medium may be a consequence of the regulatory features of AHAS. A glycyl-leucine-resistant mutant with reduced TD activity secreted a reduced amount of isoleucine and an increased amount of valine, which might be a result of the reduced rate of synthesis of the isoleucine precursor alpha-ketobutyrate and of a consequent preferential carbon flow through the valine branch of the pathway.     

De novo epileptic confusional status in a patient with cobalamin deficiency

A 80-year-old man with cobalamin deficiency and no history of epilepsy developed a partial complex epileptic confusional status (ECS) unresponsive to acute i.v. diazepam. Brain CT scan and MRI investigation ruled out a focal cerebral lesion. Therapy with high doses (10,000 micrograms i.m. daily) of cobalamin alone was started, and the patient fully recovered in the following 72-hour. Control EEGs repeatedly performed days and weeks later showed progressive disappearance of the frontal interictal spiking, while the patient was on monotherapy with cobalamin (5,000 micrograms i.m. weekly). A month later the patient unfortunately discontinued replacement therapy and 13 weeks later he developed a fatal convulsive epileptic status. To our knowledge the association of ECS and cobalamin deficiency has not been previously reported.