Chest wall deformities and thoracic scoliosis after costal cartilage graft harvesting

Donor-site complications, specifically chest wall deformities and thoracic scoliosis, occurring after harvest of costal cartilage grafts are presented and discussed. The cases of 18 patients (12 male and 6 female), who underwent costal cartilage grafts for microtia reconstruction from 1975 to 1993, were reviewed for donor-site complications using radiography and physical examination. Ribs from which costal cartilage had been harvested showed increased inward bowing on radiographs in 16 of 32 donor sites. The frequency of rib deformity in donor sites was 20.0 percent when cartilages were harvested from patients older than 10 years of age, whereas it was 63.6 percent in patients younger than 10 years old. This difference was statistically significant (p = 0.027, Fisher's exact test), although only 32 grafts were performed in 18 cases. The upper ribs demonstrate a higher incidence of deformity than lower ribs. Thoracic scoliosis was found in 4 of 16 cases. The biomechanical impact of these deformities was considered because of respiratory movement of the thorax and injury to the germinal growth center of the ribs. We recommend delaying costal cartilage grafts for as long as possible, leaving the costochondral junction intact to minimize chest wall deformity and thoracic scoliosis.     

Anticipation and imprinting in schizophrenia

Anticipation, i.e., a decrease in the age of onset and/or an increase in the severity of a disease in subsequent generations, and imprinting, i.e., different modes of parental transmission, have been suggested in trinucleotide repeat amplification diseases. The purpose of this study was to determine if anticipation and imprinting are associated with familial schizophrenia. Two generations of 49 schizophrenics from 24 families were studied. Ages of onset, numbers hospitalized, diagnostic subclasses of schizophrenia, amounts of antipsychotics, positive symptoms, negative symptoms, treatment resistance, and clinical course ratings, were compared between the two generations. The age of onset was significantly lower in the offspring generation, although there was no difference in the severity between the two generations. The negative symptom scores and clinical course scores in the offspring generation for paternal transmission were significantly higher than those for maternal transmission. Our results suggest the presence of imprinting and anticipation in schizophrenia.     

Kinetic analysis of benzyl alcohol oxidation by alcohol oxidase in aqueous—organic two-phase systems

Alcohol oxidase-catalyzed oxidation of benzyl alcohol to benzaldehyde was studied in three two-liquid phase systems involving butyl acetate, xylene and n-decane. A more highly hydrophobic solvent resulted in more favorable partitioning of the substrate into the aqueous phase and less deleterious effect on the enzyme activity. As a result, the overall production of aldehyde was highest with the n-decane system and lowest with the butyl acetate system. In addition, the organic solvent served as an extractant for the product, which acted both as a competitive inhibitor and as a denaturant of the enzyme. The time courses of benzaldehyde production were simulated by a simple mathematical model assuming Michaelis–Menten kinetics in the aqueous phase, enzyme deactivation by organic solvents and the equilibrium partitioning of the reagents between the two phases.     

Barium Titanate Ceramics with High Piezoelectricity Fabricated from Fibrous Particles

Barium titanate ceramics with high degrees of c-axis orientation were fabricated from fibrous particles. The maximum piezoelectric constant, d₃₃, obtained was ≅270 pC/N, which is much higher than values reported previously.     

Kidney transplantation and liaison psychiatry, part I: anxiety before, and the prevalence rate of psychiatric disorders before and after, transplantation

We examined psychiatric problems before and after kidney transplantation in a sample of 36 patients with end-stage renal failure. The prevalence rate of psychiatric disorders was 11.1% (4 of 36 cases) before the transplantation and 36.1% (13 of 36 cases) within 2 months after the transplantation. Except for a patient with schizophrenic disorder, no patients were found to have a psychiatric disorder from 2 to 6 months after the transplantation. In this study, we also examined anxieties and/or conflicts related to the transplantation using the synthetic house-tree-person (HTP) drawing test, a measure of mood states by means of a non-verbal expression method. The upper part of the tree trunk was not drawn in 25% of this sample (5 of 20 cases). In the HTP drawing tests immediately after the transplantation, however, trees missing the upper part of trunk were not drawn. Based on these findings, we discussed psychiatric problems in kidney transplantation.     

A study of the effects of antidepressants on the GABAA receptor and its complex based on the drug actions on the power-spectral changes of rat hippocampal EEG induced by GABA antagonists and inverse agonists

A randomized clinical trial was conducted to compare the early development of children with d-transposition of the great arteries (N = 171) repaired by an arterial switch operation that used either predominantly total circulatory arrest or predominantly continuous low-flow cardiopulmonary bypass. The children assigned to the circulatory arrest group scored lower than those assigned to low-flow bypass on the Bayley Scales of Infant Development at 1 year of age. Responses to parental questionnaires completed when the children were 2.5 years old indicated that the children in the circulatory arrest group, especially those with a ventricular septal defect, also manifested poorer expressive language and were considered to display more internalizing and externalizing problem behaviors. The use of circulatory arrest to protect vital organs during open heart surgery might place children at increased risk of early developmental difficulties, especially in the domains of motor and language function.     

Changes in riparian vegetation communities below a large dam in a monsoonal region: Futase Dam,Japan

This paper examines the effects of a dam reservoir flood control on riparian plant communities in an Asian monsoon area subjected to large summer floods under natural conditions. We compared riparian plant communities downstream of the Futase Dam located on the Arakawa River, with communities on the Nakatsu River, a tributary of the Arakawa River with no dam. Historical changes in spatial distribution of riparian vegetation communities were analysed using aerial photographs. Stand ages and flood history were also examined to analyse the likelihood of tree erosion in scenarios with and without flood control. Comparison of the two reaches shows that the reduced flood peaks below the Futase Dam led to the expansion of riparian forest area and an increase in species diversity. Reduction in the flood peaks below the dam means that the toppling moment of the trees is no longer exceeded. The result is that the riparian forest below the dam is able to develop into later successional stages. Copyright © 2004 John Wiley & Sons, Ltd.     

The effect of palatability and feeding conditions on digestive functions in rats

OBJECTIVE: Information concerning various post prandial gastrointestinal functions modulated by taste stimulation is rather limited. Thus, effects of different types of palatability and feeding condition on digestive functions were studied. METHODS: Male Sprague-Dawley rats were divided into two different feeding groups, meal feeding and ad libitum. In meal feeding, the daily feeding period was shortened gradually and was restricted eventually only 3 hours. In ad libitum feeding, food was freely available for 24 hours until experiment. After each rat in both feeding groups was fed 8.29 mM saccharin or 1.28 mM quinine flavored diet as a taste stimulant for 15 minutes, changes of gastric and intestinal nitrogen and pH and pancreatic amylase activities were compared with meal feeding vs ad libitum feeding. RESULTS: In meal feeding, no difference was observed between before and after taste stimulation or between saccharin and quinine flavored diets on gastric and intestinal nitrogen contents, pH in the stomach and in the small intestine, and amylase activity. On the other hand, in ad libitum feeding, intestinal nitrogen contents tended to stagnate until 60 minutes after tasting quinine, although there was little difference between saccharin and quinine diets on gastric nitrogen contents. Gastric juice was hardly secreted before tasting (pH 6.2) and then pH in the stomach changed to become more acid following taste stimulation. Although amylase secreted in small intestine was hardly secreted before tasting, activities after tasting saccharin were increased immediately and lasted until 120 minutes. Amylase activity in rats fed the quinine diet, however, showed slower increase and faster recovery than that in rats fed the saccharin diet. CONCLUSIONS: The results confirmed that various postingestive functions after taste stimulation depend on feeding conditions and it suggests that the ad libitum feeding condition is more appropriate than meal feeding on the experiment related to gustatory response.     

Analysis of cell damage and proliferation in Helicobacter pylori-infected human gastric mucosa from patients with gastric adenocarcinoma

Helicobacter pylori (HP) infection, a cause of multifocal atrophic gastritis, is considered an important factor related to the evolution of the human gastric mucosa from normal to intestinal-type adenocarcinoma. We examined cell proliferation and both double and single strand DNA damage in situ in 35 patients undergoing gastrectomy for adenocarcinoma with HP-infected gastric mucosa by immunolocalization of Ki-67, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling, and in situ nick translation. We also studied the distribution of intraepithelial neutrophils by elastase immunolocalization. HP infection was confirmed in all cases by serum anti-HP antibodies, ureas testing, and histopathological examination. HP-infected gastric mucosa was classified according to the degree of inflammation and intestinal metaplasia. Ki-67, terminal deoxynucleotidyl transferase-mediated labeling, in situ nick translation, and intraepithelial neutrophil indices all increased with the progression of gastritis and were highest in glands with incomplete intestinal metaplasia. All indices were lowest in gastric glands with complete intestinal metaplasia. Significant positive correlations were observed among these markers. Increased proliferative activity in HP-associated chronic gastritis in response to cell damage or injury was clearly demonstrated, suggesting that both HP-associated toxins and intraepithelial neutrophils are important in HP-related gastric epithelial injury. Increased cell turnover associated with incomplete intestinal metaplasia may result in DNA instability and subsequent development of intestinal-type gastric adenocarcinoma in HP-infected mucosa.