Impact of commercial preharvest transportation and holding on the prevalence of Salmonella enterica in cull sows

The objective of this study was to examine the prevalence of Salmonella enterica in cull sows at various stages from the farm to the abattoir. Cull sows (n=181) were sampled over 10 weeks. Fecal samples (10 g each) were collected on the farm ca. 24 h before loading and at the live-hog market ca. 3 h before loading. Samples (ileocecal lymph nodes, cecal contents, feces from the transverse colon, ventral thoracic lymph nodes, subiliac lymph nodes, sponge swabs of the left and right carcass sections, and chopped meat) were collected at the abattoir. The percentages of positive fecal samples on the farm and at the live-hog market were 3% (5 of 181 samples) and 2% (3 of 181 samples), respectively. After transport from the live-hog market (10 h) and holding at the abattoir (6 h), 41% (74 of 180) of cull sows yielded S. enterica in one or more sampled tissues. The isolation rate for total cecal contents (33%; 60 of 180 samples) was significantly (P<0.05) higher than those for ileocecal lymph nodes (7%; 12 of 181 samples), feces (11%; 20 of 181 samples), and ventral thoracic and subiliac lymph nodes (2%; 4 of 181 samples). Before a 2% lactic acid carcass wash (lasting 8 to 9 s), 14% (25 of 180) of carcasses were positive, compared with 7% (12 of 179) after the wash (P<0.05). Two S. enterica serotypes, Derby and Infantis, were found on the farm and at the live-hog market. At the abattoir, 12 serotypes that had not previously been found on the farm or at the live-hog market were recovered. The results of this study demonstrate that transport and holding practices may contribute to an increase in S. enterica infection prior to slaughter to levels much higher than those found on the farm.     

Analysing transformation products of herbicide residues in environmental samples

High-performance liquid chromatography (HPLC) methods were developed for the optimised determination of five herbicide residues (dichlorprop, isoproturon, mecoprop, metsulfuron-methyl and 2,4,5-T) and major metabolites. These compounds represent important groups of herbicides and several residues have been found as contaminants in groundwater. The methods make it possible to study these herbicides and several transformation products through simultaneous detection and quantification. Culture media as well as cleaned up extracts from sediment and groundwater can be analysed. Using HPLC with UV detection the general limit of quantification was 1.8 ng injected corresponding to a detection limit of 1-2 micrograms/l when analysing a cleaned up extract from a 20 ml water sample. The method was verified by analysing herbicide residues in groundwater collected from a wetland area. Cleaning up 20 ml groundwater with a residue level of 25 micrograms/l the general recovery was within 58-82%.     

Single tree species classification with a hypothetical multi-spectral satellite

The requirements for high resolution multi-spectral satellite images to be used in single tree species classification for forest inventories are investigated, especially with respect to spatial resolution, sensor noise and geo-registration. In the hypothetical setup, a 3D tree crown map is first obtained from very high resolution panchromatic aerial imagery and subsequently each crown is classified into one of a set of known tree species such that the difference between a model multi-spectral image generated from the 3D crown map and an acquired multi-spectral satellite image of the forested area is minimized. The investigation is conducted partly by generating synthetic data from a 3D crown map from a real mixed forest stand and partly on hypothetical high resolution multi-spectral satellite images obtained from very high resolution colour infrared aerial photographs, allowing different hypothetical spatial resolutions. Conclusions are that until a new generation of even higher resolution satellites becomes available, the most feasible source of remote sensing data for single tree classification will be aerial platforms.     

A Transmitter Architecture Based on Delta–Sigma Modulation and Switch-Mode Power Amplification

This brief presents a method of deploying RF switch-mode power amplification for varying envelope signals. Thereby the power amplifier can be operated as a switch with a high power efficiency as the result. The key idea is to transmit either a full RF period or none at all in such a way that the correct modulated RF signal is obtained after filtering. This is accomplished in a novel configuration of a low-pass DeltaSigma modulator using a phase modulated clock combined with a simple AND-gate. The designed modulator is easy to implement, displays very good linearity and offers time domain signals that promote the power efficiency of the power amplifier. The working principle is described through theory and simulations, and validation is done via measurements on a prototype of the modulator. Measurements on the prototype show that the presented modulator modulates a UMTS signal with more than 10-dB margin to the spectrum mask and EVM below 0.85% RMS (req<17.5%). Delta-sigma, power amplifier (PA), RF, switch mode, transmitter architecture, varying envelope.     

Online multi-coloring on the path revisited

Multi-coloring on the path is a model for frequency assignment in linear cellular networks. Two models have been studied in previous papers: calls may either have finite or infinite duration. For hexagonal networks, a variation of the models where limited frequency reassignment is allowed has also been studied. We add the concept of frequency reassignment to the models of linear cellular networks and close these problems by giving matching upper and lower bounds in all cases. We prove that no randomized algorithm can have a better competitive ratio than the best deterministic algorithms. In addition, we give an exact characterization of the natural greedy algorithms for these problems. All of the above results are with regard to competitive analysis. Taking steps towards a more fine-grained analysis, we consider the case of finite calls and no frequency reassignment and prove that, even though randomization cannot bring the competitive ratio down to one, it seems that the greedy algorithm is expected optimal on uniform random request sequences. We prove this for small paths and indicate it experimentally for larger graphs.     

The environmental paradox in generation: How South America is gradually becoming more dependent on thermal generation

There has been an increasing focus on global warming, emission of green house gases (GHG), and the problems this might create. In this article, we review the trend in sustainable and renewable electricity generation in South America, where the generation portfolio increasingly depends on thermal generation, in particular gas. South America is a region that has relatively low emissions, but the current development is not desirable in environmental terms. We analyze the underlying reasons for this development, which is related to security of supply, deregulation, and the cost of renewable energy. We review and discuss the policies to promote renewables in the region. We analyze the potential advantages and drawbacks of different types of market interventions, such as direct subsidies that create potentially strong market distortions, more sophisticated market interventions that might be less intrusive but not necessarily as effective as, e.g. firm energy markets. We also review market-based solutions such as the Clean Market Mechanism and its potential, and the use of renewable electricity in non-interconnected zones, which might be one of the most economically attractive applications of renewables. However, without a stronger and more aggressive intervention from the governments in the region it is unlikely that the increase in thermal generation can be stopped.     

Global spatial sampling with isotropic virtual planes: estimators of length density and total length in thick, arbitrarily orientated sections

Existing design-based direct length estimators require random rotation around at least one axis of the tissue specimen prior to sectioning to ensure isotropy of test probes. In some tissue it is, however, difficult or even impossible to define the region of interest, unless the tissue is sectioned in a specific, nonrandom orientation. Spatial uniform sampling with isotropic virtual planes circumvents the use of physically isotropic or vertical sections. The structure that is contained in a thick physical section is investigated with software-randomized isotropic virtual planes in volume probes in systematically sampled microscope fields using computer-assisted stereological analysis. A fixed volume of 3D space in each uniformly sampled field is probed with systematic random, isotropic virtual planes by a line that moves across the computer screen showing live video images of the microscope field when the test volume is scanned with a focal plane. The intersections between the linear structure and the virtual probes are counted with columns of two dimensional disectors.
Global spatial sampling with sets of isotropic uniform random virtual planes provides a basis for length density estimates from a set of parallel physical sections of any orientation preferred by the investigator, i.e. the simplest sampling scheme in stereology. Additional virtues include optimal conditions for reducing the estimator variance, the possibility to estimate total length directly using a fractionator design and the potential to estimate efficiently the distribution of directions from a set of parallel physical sections with arbitrary orientation.
Other implementations of the basic idea, systematic uniform sampling using probes that have total 3D × 4π freedom inside the section, and therefore independent of the position and the orientation of the physical section, are briefly discussed.