Effect of green manure and supplemental fertility amendments on selected soil quality parameters in an organic potato rotation in Eastern Canada

The effects of green manure, crop sequence and off-farm composts on selected soil quality parameters were assessed in a three-year organic potato (Solanum tuberosum L.) rotation in Eastern Canada. Three crop sequences varying in preceding green manure [red clover (RCl) + RCl, and beans/buckwheat or carrots + oats/peas/vetch mixture (OPV)] as main plots and four fertility treatments applied in the potato phase only [control; inorganic fertilizer; municipal solid waste compost (MSW); composted paper mill biosolid (PMB)] as subplots were compared. In 2008 and 2010, changes in selected soil quality parameters (0–15 cm) were assessed prior to planting of potatoes and at potato tuber initiation stage. Potentially mineralizable nitrogen (N) and the acid phosphatase enzyme activity average values across years were greater following RCl (1.51 abs and 622 kg ha^?1) compared with OPV (1.32 abs and 414 kg ha^?1) at potato planting. Soil NO₃–N average value was greater following RCl compared with OPV (63 vs. 52 kg ha^?1) at tuber initiation. For the other measured parameters, OPV and RCl were similar. The soil organic carbon (C) and particulate organic matter-C were greater under PMB and MSW (31.1 and 7.57 kg ha^?1) compared with fertilizer treatment (27.9 and 6.05 kg ha^?1). The microbial biomass C and microbial biomass quotient were greater under MSW (216 kg ha^?1 and 0.73 %) than PMB and fertilizer (147 kg ha^?1 and 0.50 %) across crop rotations. Annual legume green manures and off-farm composts can be used to satisfy potato N requirement and maintains soil quality in organic potato rotations.     

Test of a micromegas detector with helium-based gas mixtures for active target time projection chambers utilizing radioactive isotope beams

A Micromegas detector was tested in a helium-carbon dioxide mixture at atmospheric pressure. A stable operation of the detector was confirmed at a gain of over 10³ for a standard alpha source of ²⁴¹Am. In order to realize a position-dependent gain, an anode strip was biased while keeping the other strips at the ground potential. The gain of the biased strip was reduced by one order of magnitude without affecting the gains of the neighboring strips. The energy spectra at 3.7 MeV, the energy deposited by Am alpha particles in the sensitive region of the Micromegas, were obtained from the total charge and the charge deposit profile along the track. The energy resolutions for helium:carbon dioxide 90:10 mixture at a gas gain of about 100 were 11% FWHM for the former and 4% FWHM for the latter. The present results highlight the Micromegas as a promising electron amplifier of the forthcoming active target time projection chambers that will be dedicated to nuclear reactions with radioactive isotope beams.     

Glitter and glints on water

We present new observations of glitter and glints using short and long time exposure photographs and high frame rate videos. Using the sun and moon as light sources to illuminate the ocean and laboratory water basins, we found that (1) most glitter takes place on capillary waves rather than on gravity waves, (2) certain aspects of glitter morphology depend on the presence or absence of thin clouds between the light source and the water, and (3) bent glitter paths are caused by asymmetric wave slope distributions We present computer simulations that are able to reproduce the observations and make predictions about the brightness, polarization, and morphology of glitter and glints. We demonstrate that the optical catastrophe represented by creation and annihilation of a glint can be understood using both ray optics and diffraction theory.     

Stochastic estimation of arm mechanical impedance during robotic stroke rehabilitation.

This paper presents a stochastic method to estimate the multijoint mechanical impedance of the human arm suitable for use in a clinical setting, e.g., with persons with stroke undergoing robotic rehabilitation for a paralyzed arm. In this context, special circumstances such as hypertonicity and tissue atrophy due to disuse of the hemiplegic limb must be considered. A low-impedance robot was used to bring the upper limb of a stroke patient to a test location, generate force perturbations, and measure the resulting motion. Methods were developed to compensate for input signal coupling at low frequencies apparently due to human-machine interaction dynamics. Data was analyzed by spectral procedures that make no assumption about model structure. The method was validated by measuring simple mechanical hardware and results from a patient's hemiplegic arm are presented.     

The impact of peptic ulcer disease and infection with Helicobacter pylori on life expectancy

OBJECTIVE: Knowledge about the influence of H. pylori-related disease on life expectancy might affect physician behavior in dealing with such disease. The aim of this study was to assess how life expectancy is influenced by H. pylori infection and peptic ulcer disease. METHODS: The declining exponential approximation of life expectancy was used to model the effects of H. pylori and various peptic ulcer disease conditions on life expectancy. Deaths from peptic ulcer and gastric cancer were determined from the Vital Statistics of the United States. H. pylori prevalence rates were derived from the existing literature. RESULTS: Cure of active peptic ulcer increases life expectancy by 2.3 yr in persons aged 40-44 yr and 121 days in persons aged 70-74 yr. More substantial impact occurs in complicated ulcer, with increases in life expectancy ranging between 26.1 and 6.3 yr. Primary prevention of H. pylori could increase life expectancy by 190 days in those aged 40-44 yr and 26 days in 70-74-yr-old subjects. CONCLUSION: The benefit of ulcer cure or H. pylori prevention diminishes as age advances. Cure of ulcers in young patients or in those who have sustained complications results in an appreciable increase in life expectancy. Successful primary prevention of H. pylori in selected populations could substantially increase life expectancy.     

Response of two populations of holm oak (Quercus rotundifolia Lam.) to sulfur dioxide

Experiments were carried out with seedlings of Quercus rotundifolia Lam., an evergreen schlerophyllous tree typical of the Spanish Mediterranean climate environments. Fruits were collected in two distant (800 km) populations located in the center (southern Spain) and northern border (northern Spain) of the area of distribution of the species. One-month-old potted plants were grown for 130 days in an enriched atmosphere of SO2 (0.23 ppm, 14 h/day) in controlled (growth chamber) conditions. Both northern and southern plants underwent a significant decrease in growth rate as a consequence of the treatment. Even so, plants appear to be quite resistant to SO2 compared with either more temperate or more productive species. The southern population was more sensitive to the treatment, as reflected by the bigger decrease in both growth and photosynthetic rates. Differences in resistance appear to be related to the biogeographic origin of the populations studied, which underlines the importance of biogeographic aspects in studies of resistance to air pollutants.     

Crystal structure at 1.95 A resolution of the breast tumour-specific antibody SM3 complexed with its peptide epitope reveals novel hypervariable loop recognition

The anti-breast tumour antibody SM3 has a high selectivity in reacting specifically with carcinoma-associated mucin. SM3 recognises the core repeating motif (Pro-Asp-Thr-Arg-Pro) of aberrantly glycosylated epithelial mucin MUC1, and has potential as a therapeutic and diagnostic tool. Here we report the crystal structure of the Fab fragment of SM3 in complex with a 13-residue MUC1 peptide antigen (Thr1P-Ser2P-Ala3P-Pro4P-Asp5P-Thr6P -Arg7P-Pro8P-Ala9P-Pro10P-Gly11P- Ser12P-Thr13P). The SM3-MUC1 peptide structure was solved by molecular replacement, and the current model is refined at 1.95 A resolution with an R-factor of 21.3% and R-free 28.3%. The MUC1 peptide is bound both by non-polar interactions and hydrogen bonds in an elongated groove in the antibody-combining site through interactions with Complimentarity Determining Regions (CDRs), three of the light chain (L1, L2, L3) and two of the heavy chain (H1 and H3). The conformation of the peptide is mainly extended with no discernable standard secondary structure. There is a single non-proline cis-peptide bond in H3 (Val95H-Gly96H-Gln97H-Phe98H-Ala101H-Ty r102H) between Gly96H and Gln97H, which appears to play a role in SM3-peptide antigen interactions, and represents the first such example within an antibody hypervariable loop. The SM3-MUC1 peptide structure has implications for rational therapeutic and diagnostic antibody engineering.