Impact of β‐glucan and other oat flour components on physico‐chemical and sensory properties of extruded oat cereals

Whole oat flour, N979‐5‐2‐4 (N979), IA95111 (IA95), ‘Jim’ and ‘Paul’ with β‐glucan concentration of 8.6%, 7.6%, 5.3%, and 5.9%, respectively, was made into extruded breakfast cereal (EBC). EBC β‐glucan had greater peak molecular weight than corresponding whole oat flour. Both whole oat flour and EBC from N979 and IA95 had greater peak viscosity (PV), as measured using a Rapid ViscoAnalyser, than that from Jim or Paul. Bile acid binding of EBC, correlated with total β‐glucan concentrations and PV, was either similar or greater than that of corresponding whole oat flour. Jim EBC had the least expansion ratio, lowest brown colour and cereal aroma but greatest tooth packing values. Paul and N979 EBC, although different from each other in a few sensory attributes, had similar acceptability as judged from a consumer test, suggesting that oats with elevated β‐glucan concentrations can be successfully incorporated into EBC with minimal processing alterations.     

SIZE EFFECTS ON THE THERMAL CONDUCTIVITY OF THIN METALLIC WIRES: MICROSCALE IMPLICATIONS

This study examines the influence of radial thickness on the thermal conductivity of thin metallic wires. While size effects on the electrical conductivity of thin wires have been discussed in the literature, research into size effects on thermal conductivity still requires investigation. At such small length scales, the assumption that the reduced electrical conductivity can be simply related to the reduced thermal conductivity through a Wiedemann-Franz relation is subject to question. This study uses the Boltzmann transport equation for electrons to determine the thermal conductivity of a thin wire directly. Electrons are treated as the primary heat carriers in a thin wire with a thermal gradient along the axis. A single-crystal, defect-free, metallic thin wire is considered in the derivation. An expression is presented which accounts for the radial size effects on axial thermal conductivity. The derived thermal conductivity is compared to expressions for the reduced electrical conductivity, and the applicability of the Wiedemann-Franz relation is discussed.     

Treatment of milk by gamma irradiation—effect of anoxia on lipid peroxidation and the survival of Pseudomonas aeruginosa

One of the causes of organoleptic changes in milk, lipid peroxidation, has been monitored by a sensitive assay newly applied to foodstuffs. It has been shown that even at medium radiation doses (up to 3600 Gy) negligible lipid peroxidation takes place, providing the milk is thoroughly gassed with nitrogen. Using a test spoilage microorganism, Pseudomonas aeruginosa (Schroeter), it was shown that the D₁₀ was 35 Gy in air and 105 Gy under anaerobic conditions. Dose/survival curves indicate that 5 decades of killing needed 200 Gy in air and 600 Gy under nitrogen. However, even after receiving a dose of 1600 Gy, both aerobically and anaerobically treated milk failed the methylene blue test at 21 days post-irradiation. The data indicate that, although the deleterious lipid peroxidation effects of irradiation can be minimised by the removal of air, this in turn results in a degree of protection being afforded to the bacteria, and that doses in excess of 1600 Gy are needed to effectively sterilise milk.     

Perceptual identification of contextual attributes and infant memory retrieval.

The contribution of specific contextual attributes to recognition of a well-learned cue was examined in 4 experiments with 6-mo-olds. 24 hrs after learning to move a given mobile in a distinctive visual surround by kicking, recognition of the training cue was tested in either the original context or in one in which only a single contextual attribute was altered. Retrieval was completely disrupted by all form changes involving the deletion of angles and by a chromatic figure/ground reversal, but a discriminable change in form color had no effect. Although infants displayed partial retention in a degraded context after 1 day, they displayed no retention when a reminder was administered in the same degraded context after 20 days. These data reveal that infants do not encode contextual information holistically; moreover, they imply a privileged status for highly specific information about the incidental setting in which an event occurs. Unless this same highly specific information is perceptually identified at the time of testing, the memory of that event will not be retrieved. Data are consistent with the hypothesis that the visual context serves as an initial attention gate for memory retrieval. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Social support and prognosis in patients at increased psychosocial risk recovering from myocardial infarction.

Objective: To compare the impact of network support and different types of perceived functional support on all-cause mortality or nonfatal reinfarction for patients with a recent acute myocardial infarction (AMI). Design: Participants were recruited from the Enhancing Recovery in Coronary Heart Disease (ENRICHD) trial; 2,481 AMI patients with depression or low social support were randomized to a cognitive-behavioral intervention or to a usual care control group. Data collection for certain measures of social support was limited: 2,466 participants completed the ENRICHD Social Support Inventory; 2,457 completed the Perceived Social Support Scale; 1,296 completed the Social Network Questionnaire; and 707 completed the Interpersonal Support and Evaluation List, Tangible Support subscale. Patients also completed the Beck Depression Inventory and were followed for up to 4.5 years. Main Outcome Measure: Time to death or nonfatal reinfarction. Results: Over the follow-up period, 599 patients (24%) died or had a nonfatal AMI. Survival models controlling age, sex, race, socioeconomic status, smoking, antidepressant use, and a composite measure of increased risk revealed that higher levels of perceived social support were associated with improved outcome for patients without elevated depression but not for patients with high levels of depression. Neither perceived tangible support nor network support were associated with more frequent adverse events. Conclusion: AMI patients should be assessed for multiple dimensions of perceived functional support and depression to identify those at increased psychosocial risk who may benefit from treatment. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

甲烷市场化合作计划——煤矿煤层气项目开发的机遇

煤矿煤层气的排放是全球甲烷排放的一个重要来源，“甲烷市场化合作计划”的发起是为了识别并开发相关的工程项目，减少煤矿、垃圾填埋场和石油天然气系统等各领域中的甲烷排放。煤矿煤层气项目是“甲烷市场化合作计划”的三个核心领域之一，对推动煤炭行业的甲烷减排发挥重要作用。     

Immune and Anticancer Responses Elicited by Fully Synthetic Aberrantly Glycosylated MUC1 Tripartite Vaccines Modified by a TLR2 or TLR9 Agonist

The mucin MUC1 is overexpressed and aberrantly glycosylated by many epithelial cancer cells manifested by truncated O‐linked saccharides. Although tumor‐associated MUC1 has generated considerable attention because of its potential for the development of a therapeutic cancer vaccine, it has been difficult to design constructs that consistently induce cytotoxic T‐lymphocytes (CTLs) and ADCC‐mediating antibodies specific for the tumor form of MUC1. We have designed, chemically synthesized, and immunologically examined vaccine candidates each composed of a glycopeptide derived from MUC1, a promiscuous T_helper peptide, and a TLR2 (Pam₃CysSK₄) or TLR9 (CpG‐ODN 1826) agonist. It was found that the Pam₃CysSK₄‐containing compound elicits more potent antigenic and cellular immune responses, resulting in a therapeutic effect in a mouse model of mammary cancer. It is thus shown, for the first time, that the nature of an inbuilt adjuvant of a tripartite vaccine can significantly impact the quality of immune responses elicited against a tumor‐associated glycopeptide. The unique adjuvant properties of Pam₃CysSK₄, which can reduce the suppressive function of regulatory T cells and enhance the cytotoxicity of tumor‐specific CTLs, are likely responsible for the superior properties of the vaccine candidate 1 .     

Discovery of 5′′‐Chloro‐N‐[(5,6‐dimethoxypyridin‐2‐yl)methyl]‐2,2′:5′,3′′‐terpyridine‐3′‐carboxamide (MK‐1064): A Selective Orexin 2 Receptor Antagonist (2‐SORA) for the Treatment of Insomnia

The field of small‐molecule orexin antagonist research has evolved rapidly in the last 15 years from the discovery of the orexin peptides to clinical proof‐of‐concept for the treatment of insomnia. Clinical programs have focused on the development of antagonists that reversibly block the action of endogenous peptides at both the orexin 1 and orexin 2 receptors (OX₁R and OX₂R), termed dual orexin receptor antagonists (DORAs), affording late‐stage development candidates including Merck’s suvorexant (new drug application filed 2012). Full characterization of the pharmacology associated with antagonism of either OX₁R or OX₂R alone has been hampered by the dearth of suitable subtype‐selective, orally bioavailable ligands. Herein, we report the development of a selective orexin 2 antagonist (2‐SORA) series to afford a potent, orally bioavailable 2‐SORA ligand. Several challenging medicinal chemistry issues were identified and overcome during the development of these 2,5‐disubstituted nicotinamides, including reversible CYP inhibition, physiochemical properties, P‐glycoprotein efflux and bioactivation. This article highlights structural modifications the team utilized to drive compound design, as well as in vivo characterization of our 2‐SORA clinical candidate, 5′′‐chloro‐N‐[(5,6‐dimethoxypyridin‐2‐yl)methyl]‐2,2′:5′,3′′‐terpyridine‐3′‐carboxamide (MK‐1064), in mouse, rat, dog, and rhesus sleep models.     

PARAFAC modeling of three-way hyperspectral images: Endogenous fluorophores as health biomarkers in aquatic species

Demand for a renewable liquid-phase fuel has reinvigorated interest in the development of novel biofuels from algae. The effects of fluctuating nutrients, temperature, and predation which arise in algal aquaculture necessitate novel analytical methods to assess and optimize the health of these cultures. In this article we describe the use of trilinear modeling to these ends, using imaging data of endogenous fluorescence from the marine diatom, Thalassiosira pseudonana, collected on a hyperspectral fluorescence confocal microscope (HSF-CM). By combining the spatial and spectral data with the additional physical parameter introduced by the varying photobleach rates of endogenous fluorophores, we obtain a means for employing trilinear analysis in this system. In this case, analysis was implemented using the method of parallel factors analysis (PARAFAC) which alleviates the rotational ambiguities involved in multivariate analysis of 2-way data. By imposing a nutrient limitation in our diatom culture, and collecting single cell microscopy data at various time points during the nutritional stress, PARAFAC results reveal evolution of the spectral emission characteristics of six components and a background as a result of incremental photobleaching. Although the underlying mechanisms for these observations are not addressed, the differences observed between nutritively normal and nutritively stressed cells provide signatures for early detection of health deterioration in the algal culture. Finally, the combination of hyperspectral fluorescence microscopy with PARAFAC is generally applicable for the study of complex biological systems with endogenous fluorescence.