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71.
72.
Chen Yanni Liu Liqiang Xu Liguang Song Shanshan Kuang Hua Cui Gang Xu Chuanlai 《Nano Research》2017,10(8):2833-2844
A gold immunochromatographic sensor (GICS) was developed for the rapid detection of 26 sulfonamides in honey samples.The sensor was based on a group-specific monoclonal antibody (mAb) that can recognize all 26 sulfonamides.Three haptens (hapten 1 with a thiazole ring,hapten 2 with a benzene ring,and hapten 3 with a straight carbon chain) were used for antigen preparation.With hybridoma technology,a group-specific mAb was screened with a 50% maximal inhibitory concentration (IC50) against sulfathizole (STZ) and the other 25 analogues ranging from 0.08 to 90.18 ng/mL.Mono-dispersed gold nanoparticles were conjugated with the mAb to develop the lateral immunochromatographic strip.A labeled antibody concentration of 0.1 μg/mL and a coating antigen concentration of 0.2 μg/mL in the test line were chosen for strip preparation.Under optimized conditions,the visual limits of detection (vLOD) for the concentrations of STZ,sulfamethoxazole,sulfamethizole,sulfadiazine,sulfamerazine,sulfadimethoxine,sulfamonomethoxine,sulfameter,sulfamethoxypyridazine,and sulfachloropyridazine were 5,0.25,0.25,10,5,10,25,2.5,5,0.25,and 10 μg/kg,respectively.Scanner analysis in honey samples revealed good performance for detection of the 26 sulfonamides.Commercial honey samples were tested with the sensor and positive results were confirmed with high-performance liquid chromatography.The proposed strip sensor provides a convenient method for the rapid and reliable determination of sulfonamides pollutants in honey samples. 相似文献
73.
Juan Peng Liqiang Liu Liguang Xu Shanshan Song Hua Kuang Gang Cui Chuanlai Xu 《Nano Research》2017,10(1):108-120
For rapid and simultaneous detection of (fluoro)quinolones, a broadly specific monoclonal antibody (mAb) that recognizes 32 (fluoro)quinolone antibiotics was prepared using a mixture of a norfloxacin derivative and a sarfloxacin derivative as the hapten. An immunochromatographic strip based on gold nanoparticles (AuNPs) was then assembled with goat anti-mouse antibody and antigen (sarfloxacin coupled to ovalbumin), used to form the C line and T line, respectively. This antigen competes with the (fluoro)quinolones in a sample incubated with mAbs labeled with AuNPs. The strip can detect 32 (fluoro)quinolones including oxolinic acid, nalidixic acid, miloxacin, pipemidic acid, piromidic acid, rosoxacin, cinoxacin, norfloxacin, pefloxacin, lomfloxacin, enofloxacin, fleroxacin, ciprofloxacin, enrofloxacin, dafloxacin, orbifloxacin, sparfloxacin, gemifloxacin, besifloxacin, balofloxacin, gatifloxacin, moxifloxacin, nadifloxacin, ofloxacin, marbofloxacin, flumequine, pazufloxacin, prulifloxacin, sarafloxacin, difloxacin, trovafloxacin, and tosufloxacin in milk within 10 min with the naked eye. The cut-off values of the strip range from 1 to 100 ng/mL and the limits of detection are 0.1–10 ng/mL. The strip does not cross-react with antibiotics including tetracycline, sulfamethazine, ampicillin, erythromycin, aflatoxin B1, or gentamicin. In short, this immunochromatographic strip is a very useful tool for the primary screening of (fluoro)quinolones in milk. 相似文献
74.
Hao Wang Yuhan Wu Pengcheng Wu Shanshan Chen Xuhong Guo Guihua Meng Banghua Peng Jianning Wu Zhiyong Liu 《材料科学前沿(英文版)》2017,11(2):130-138
A novel Ag/AgCl/chitosan composite photocatalyst was successfully prepared by a simple one-step method. During this progress, environmentally benign chitosan not only served as reductant to reduce Ag+ to Ag0 species, but also acted as supporter for Ag/AgCl nanoparticles. XRD, SEM, EDX, UV-vis DRS and XPS were employed to characterize the as-prepared simples. SEM images of Ag/AgCl/chitosan composites revealed that Ag/AgCl nanoparticles were successfully loaded onto chitosan without obvious aggregation. All Ag/AgCl/chitosan composites exhibited efficient photocatalytic activity for the degradation of rhodamine B (RhB) under visible-light irradiation. The result of photocatalytic degradation experiment indicated that 20% of the mass ratio of AgCl to chitosan was the optimum, and after 40 min photocatalytic reaction, the degradation rate reached about 96%. 相似文献
75.
Guodong Wang Zhanwen Niu Shanshan Lv Liang Qu Zhen He 《Quality and Reliability Engineering International》2016,32(5):1945-1953
Because of cost and time limitations, reliability experiments frequently contain subsampling, which is a restriction on randomization. A two‐stage approach can analyze right censored Weibull distributed reliability data with subsampling. However, in implementing such a method, we found that it did not address the problems of how to perform confidence intervals of low percentiles and reduce the bias of estimates. In this paper, we present a two‐stage bootstrapping approach and an unbiasing factor approach to solve the aforementioned problems. An example is provided to illustrate the proposed method. In addition, the proposed method is compared with existing methods through simulation. The resulting simulations show that the proposed method performs well in low percentiles. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
76.
77.
建立高度卡尺示值误差测量不确定度的测量模型,对高度卡尺测量过程中引入的不确定度进行分类和量化,较为全面地评定了各个不确定度分量,并得出测量结果的扩展不确定度。 相似文献
78.
Qi Jia Xin Ou Manuel Langer Benjamin Schreiber Jörg Grenzer Pablo F. Siles Raul D. Rodriguez Kai Huang Ye Yuan Alireza Heidarian René Hübner Tiangui You Wenjie Yu Kilian Lenz Jürgen Lindner Xi Wang Stefan Facsko 《Nano Research》2018,11(7):3519-3528
A nanofabrication method for the production of ultra-dense planar metallic nanowire arrays scalable to wafer-size is presented. The method is based on an efficient template deposition process to grow diverse metallic nanowire arrays with extreme regularity in only two steps. First, III–V semiconductor substrates are irradiated by a low-energy ion beam at an elevated temperature, forming a highly ordered nanogroove pattern by a “reverse epitaxy” process due to self-assembly of surface vacancies. Second, diverse metallic nanowire arrays (Au, Fe, Ni, Co, FeAl alloy) are fabricated on these III–V templates by deposition at a glancing incidence angle. This method allows for the fabrication of metallic nanowire arrays with periodicities down to 45 nm scaled up to wafer-size fabrication. As typical noble and magnetic metals, the Au and Fe nanowire arrays produced here exhibited large anisotropic optical and magnetic properties, respectively. The excitation of localized surface plasmon resonances (LSPRs) of the Au nanowire arrays resulted in a high electric field enhancement, which was used to detect phthalocyanine (CoPc) in surface-enhanced Raman scattering (SERS). Furthermore, the Fe nanowire arrays showed a very high in-plane magnetic anisotropy of approximately 412 mT, which may be the largest in-plane magnetic anisotropy field yet reported that is solely induced via shape anisotropy within the plane of a thin film. 相似文献
79.
Yongjie Yang Shanshan Tu Raja Hashim Ali Hisham Alasmary Muhammad Waqas Muhammad Nouman Amjad 《计算机、材料和连续体(英文)》2023,74(1):801-815
With the recent developments in the Internet of Things (IoT), the amount of data collected has expanded tremendously, resulting in a higher demand for data storage, computational capacity, and real-time processing capabilities. Cloud computing has traditionally played an important role in establishing IoT. However, fog computing has recently emerged as a new field complementing cloud computing due to its enhanced mobility, location awareness, heterogeneity, scalability, low latency, and geographic distribution. However, IoT networks are vulnerable to unwanted assaults because of their open and shared nature. As a result, various fog computing-based security models that protect IoT networks have been developed. A distributed architecture based on an intrusion detection system (IDS) ensures that a dynamic, scalable IoT environment with the ability to disperse centralized tasks to local fog nodes and which successfully detects advanced malicious threats is available. In this study, we examined the time-related aspects of network traffic data. We presented an intrusion detection model based on a two-layered bidirectional long short-term memory (Bi-LSTM) with an attention mechanism for traffic data classification verified on the UNSW-NB15 benchmark dataset. We showed that the suggested model outperformed numerous leading-edge Network IDS that used machine learning models in terms of accuracy, precision, recall and F1 score. 相似文献
80.
L Tonella BJ Walsh JC Sanchez K Ou MR Wilkins M Tyler S Frutiger AA Gooley I Pescaru RD Appel JX Yan A Bairoch C Hoogland FS Morch GJ Hughes KL Williams DF Hochstrasser 《Canadian Metallurgical Quarterly》1998,19(11):1960-1971
The combination of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), computer image analysis and several protein identification techniques allowed the Escherichia coli SWISS-2DPAGE database to be established. This is part of the ExPASy molecular biology server accessible through the WWW at the URL address http://www.expasy.ch/ch2d/ch2d-top.html . Here we report recent progress in the development of the E. coli SWISS-2DPAGE database. Proteins were separated with immobilized pH gradients in the first dimension and sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the second dimension. To increase the resolution of the separation and thus the number of identified proteins, a variety of wide and narrow range immobilized pH gradients were used in the first dimension. Micropreparative gels were electroblotted onto polyvinylidene difluoride membranes and spots were visualized by amido black staining. Protein identification techniques such as amino acid composition analysis, gel comparison and microsequencing were used, as well as a recently described Edman "sequence tag" approach. Some of the above identification techniques were coupled with database searching tools. Currently 231 polypeptides are identified on the E. coli SWISS-2DPAGE map: 64 have been identified by N-terminal microsequencing, 39 by amino acid composition, and 82 by sequence tag. Of 153 proteins putatively identified by gel comparison, 65 have been confirmed. Many proteins have been identified using more than one technique. Faster progress in the E. coli proteome project will now be possible with advances in biochemical methodology and with the completion of the entire E. coli genome. 相似文献