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Stefanie Bußmann Harald Kruggel-Emden Mathis Reichert 《Advanced Powder Technology》2021,32(7):2171-2191
The particle based Discrete Element Method (DEM) can be applied to examine comminution processes. In this study, a DEM framework has been extended to model particle breakage without mass loss. After a breakage event occurs, spherical particles, as often considered in the DEM, are replaced by size reduced spherical fragments. During the following time steps, the fragments grow to their desired sizes, so that the mass loss can be counterbalanced. Previously defined overlaps with adjacent unbroken and broken particles (fragments) as well as walls are allowed. The breakage model has been realized in a parallelized DEM framework because comminution processes are often attributed to large numbers of particles and by parallelization the computational time can be reduced efficiently. An oedometer (one-dimensional compression in axial direction of a confined particle bed) has been modelled to investigate the parallelization efficiency and the influence of the permitted overlaps during the growth process on the growth duration. A simplified roller mill has been considered to examine the applicability of the breakage procedure considering parallelization. The results show that parallelization reduces computational time considerably. The breakage procedure is suitable to model comminution processes involving even densely packed particle systems and is superior to existing approaches. 相似文献
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Anne Hofmann Stefanie Wienkoop Snke Harder Fabian Bartlog Sabine Lüthje 《International journal of molecular sciences》2020,21(22)
Flooding induces low-oxygen environments (hypoxia or anoxia) that lead to energy disruption and an imbalance of reactive oxygen species (ROS) production and scavenging enzymes in plants. The influence of hypoxia on roots of hydroponically grown maize (Zea mays L.) plants was investigated. Gene expression (RNA Seq and RT-qPCR) and proteome (LC–MS/MS and 2D-PAGE) analyses were used to determine the alterations in soluble and membrane-bound class III peroxidases under hypoxia. Gel-free peroxidase analyses of plasma membrane-bound proteins showed an increased abundance of ZmPrx03, ZmPrx24, ZmPrx81, and ZmPr85 in stressed samples. Furthermore, RT-qPCR analyses of the corresponding peroxidase genes revealed an increased expression. These peroxidases could be separated with 2D-PAGE and identified by mass spectrometry. An increased abundance of ZmPrx03 and ZmPrx85 was determined. Further peroxidases were identified in detergent-insoluble membranes. Co-regulation with a respiratory burst oxidase homolog (Rboh) and key enzymes of the phenylpropanoid pathway indicates a function of the peroxidases in membrane protection, aerenchyma formation, and cell wall remodeling under hypoxia. This hypothesis was supported by the following: (i) an elevated level of hydrogen peroxide and aerenchyma formation; (ii) an increased guaiacol peroxidase activity in membrane fractions of stressed samples, whereas a decrease was observed in soluble fractions; and (iii) alterations in lignified cells, cellulose, and suberin in root cross-sections. 相似文献
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Stefanie Lochmann Yannik Bruniger Vincent Gottsmann Lydia Galle Julia Grothe Stefan Kaskel 《Advanced functional materials》2020,30(19)
A novel three‐electrode electrolyte supercapacitor (electric double‐layer capacitor [EDLC]) architecture in which a symmetrical interdigital “working” two‐electrode micro‐supercapacitor array (W‐Cap) is paired with a third “gate” electrode that reversibly depletes/injects electrolyte ions into the system controlling the “working” capacity effectively is described. All three electrodes are based on precursor‐derived nanoporous carbons with well‐defined specific surface area (735 m2 g?1). The interdigitated architecture of the W‐Cap is precisely manufactured using 3D printing. The W‐Cap operating with a proton conducting PVA/H2SO4‐hydrogel electrolyte and high capacitance (6.9 mF cm?2) can be repeatedly switched “on” and “off”. By applying a low DC bias potential (?0.5 V) at the gate electrode, the AC electroadsorption in the coupled interdigital nanoporous carbon electrodes of the W‐Cap is effectively suppressed leading to a stark capacity drop by two orders of magnitude from an “on” to an “off” state. The switchable micro‐supercapacitor is the first of its kind. This general concept is suitable for implementing a broad range of nanoporous materials and advanced electrolytes expanding its functions and applications in future. The integration of intelligent functions into EDLC devices has extensive implications for diverse areas such as capacitive energy management, microelectronics, iontronics, and neuromodulation. 相似文献
89.
Microfluidic Templated Multicompartment Microgels for 3D Encapsulation and Pairing of Single Cells 下载免费PDF全文
Liyuan Zhang Kaiwen Chen Haoyue Zhang Bo Pang Chang‐Hyung Choi Angelo S. Mao Hongbing Liao Stefanie Utech David J. Mooney Huanan Wang David A. Weitz 《Small (Weinheim an der Bergstrasse, Germany)》2018,14(9)
Controlled encapsulation and pairing of single cells within a confined 3D matrix can enable the replication of the highly ordered cellular structure of human tissues. Microgels with independently controlled compartments that can encapsulate cells within separately confined hydrogel matrices would provide precise control over the route of pairing single cells. Here, a one‐step microfluidic method is presented to generate monodisperse multicompartment microgels that can be used as a 3D matrix to pair single cells in a highly biocompatible manner. A method is presented to induce microgels formation on chip, followed by direct extraction of the microgels from oil phase, thereby avoiding prolonged exposure of the microgels to the oil. It is further demonstrated that by entrapping stem cells with niche cells within separate but adjacent compartments of the microgels, it can create complex stem cell niche microenvironments in a controlled manner, which can serve as a useful tool for the study of cell–cell interactions. This microfluidic technique represents a significant step toward high‐throughput single cells encapsulation and pairing for the study of intercellular communications at single cell level, which is of significant importance for cell biology, stem cell therapy, and tissue engineering. 相似文献
90.
Jasmina Ga
anin Jana Hedrich Stefanie Sieste Gunnar Glaßer Ingo Lieberwirth Corinna Schilling Stephan Fischer Holger Barth Bernd Knll Christopher V. Synatschke Tanja Weil 《Advanced materials (Deerfield Beach, Fla.)》2019,31(2)
The synthesis of hybrid hydrogels by pH‐controlled structural transition with exceptional rheological properties as cellular matrix is reported. “Depsi” peptide sequences are grafted onto a polypeptide backbone that undergo a pH‐induced intramolecular O–N–acyl migration at physiological conditions affording peptide nanofibers (PNFs) as supramolecular gelators. The polypeptide–PNF hydrogels are mechanically remarkably robust. They reveal exciting thixotropic behavior with immediate in situ recovery after exposure to various high strains over long periods and self‐repair of defects by instantaneous reassembly. High cytocompatibility, convenient functionalization by coassembly, and controlled enzymatic degradation but stability in 2D and 3D cell culture as demonstrated by the encapsulation of primary human umbilical vein endothelial cells and neuronal cells open many attractive opportunities for 3D tissue engineering and other biomedical applications. 相似文献