Estimation of low bacterial concentration: Listeria monocytogenes in raw milk

A time-series bacteriological analysis has been carried out on milk collected on farms from 1997 to 2001 by a plant producing raw milk soft cheese, with the purpose of assessing the time course of the presence/absence of Listeria monocytogenes. A standard data collection procedure was used, in which farms were tested on a monthly or biweekly basis and 2-3 days after the detection of milk tanker contamination. This procedure yielded low figures for contamination frequencies. The average value and the median of the monthly prevalence of farms detected positive for L. monocytogenes were 2.4 and 0%, respectively. A seasonal effect (with peaks in winter) was observed. Between 1997 and 2001, there was no significant decrease of contamination rates, in spite of the efforts on the contaminated farms. Over the last year of the study (from March 2000 to February 2001), a new data collection procedure was implemented that allowed much better detection of sporadic occurrences. Milk samples were collected from the bulk tank of each participating farm just before pick-up, then stored and subsequently analysed whenever the milk tanker was found contaminated. The average value and the median of the monthly prevalence of positive farms were found equal to 7.7 and 0%, respectively (for a mean prevalence of L. monocytogenes in the milk tanker of 3.2%). These results confirm that farm milk contamination is, most often, a sporadic event In addition to this prevalence study, contamination levels were quantified by enumerating L. monocytogenes using direct plating of small volumes of farm milk previously tested positive. Most often, these levels were extremely low. A simple simulation model shows that, when milk tankers were found positive, contamination levels in the corresponding bulk-tank milk are themselves very low (typically, below 3 L. monocytogenes per millilitre with most probable concentration 0.1 Colony Forming Unit (CFU)/ml and median ranging from 5.10(-2) to 0.1 CFU/ml). Such low levels are very likely to be due to environmental contamination.     

A Novel Stochastic Game Via the Quantitative μ-calculus

The quantitative μ-calculus qMμ extends the applicability of Kozen's standard μ-calculus [D. Kozen, Results on the propositional μ-calculus, Theoretical Computer Science 27 (1983) 333–354] to probabilistic systems. Subsequent to its introduction [C. Morgan, and A. McIver, A probabilistic temporal calculus based on expectations, in: L. Groves and S. Reeves, editors, Proc. Formal Methods Pacific '97 (1997), available at [PSG, Probabilistic Systems Group: Collected reports, http://web.comlab.ox.ac.uk/oucl/research/areas/probs/bibliography.html]; also appears at [A. McIver, and C. Morgan, “Abstraction, Refinement and Proof for Probabilistic Systems,” Technical Monographs in Computer Science, Springer, New York, 2005, Chap. 9], M. Huth, and M. Kwiatkowska, Quantitative analysis and model checking, in: Proceedings of 12th annual IEEE Symposium on Logic in Computer Science, 1997] it has been developed by us [A. McIver, and C. Morgan, Games, probability and the quantitative μ-calculus qMu, in: Proc. LPAR, LNAI 2514 (2002), pp. 292–310, revised and expanded at [A. McIver, and C. Morgan, Results on the quantitative μ-calculus qMμ (2005), to appear in ACM TOCL]; also appears at [A. McIver, and C. Morgan, “Abstraction, Refinement and Proof for Probabilistic Systems,” Technical Monographs in Computer Science, Springer, New York, 2005, Chap. 11], A. McIver, and C. Morgan, “Abstraction, Refinement and Proof for Probabilistic Systems,” Technical Monographs in Computer Science, Springer, New York, 2005, A. McIver, and C. Morgan, Results on the quantitative μ-calculus qMμ (2005), to appear in ACM TOCL] and by others [L. de Alfaro, and R. Majumdar, Quantitative solution of omega-regular games, Journal of Computer and System Sciences 68 (2004) 374–397]. Beyond its natural application to define probabilistic temporal logic [C. Morgan, and A. McIver, An expectation-based model for probabilistic temporal logic, Logic Journal of the IGPL 7 (1999), pp. 779–804, also appears at [A. McIver, and C. Morgan, “Abstraction, Refinement and Proof for Probabilistic Systems,” Technical Monographs in Computer Science, Springer, New York, 2005, Chap.10]], there are a number of other areas that benefit from its use.One application is stochastic two-player games, and the contribution of this paper is to depart from the usual notion of “absolute winning conditions” and to introduce a novel game in which players can “draw”.The extension is motivated by examples based on economic games: we propose an extension to qMμ so that they can be specified; we show that the extension can be expressed via a reduction to the original logic; and, via that reduction, we prove that the players can play optimally in the extended game using memoryless strategies.     

Hyperbranched Rigid Aromatic Phosphorus-Containing Flame Retardants for Epoxy Resins

A rigid aromatic phosphorus-containing hyperbranched flame retardant structure is synthesized from 10-(2,5-dihydroxyphenyl)-10H-9-oxa-10-phosphaphenanthrene-10-oxide (DOPO-HQ), tris(4-hydroxyphenyl)phosphine oxide (THPPO), and 1,4-terephthaloyl chloride (TPC). The resulting poly-(DOPO-HQ/THPPO-terephthalate) (PDTT) is implemented as a flame retardant into an epoxy resin (EP) at a 10 wt% loading. The effects on EP are compared with those of the monomer DOPO-HQ and triphenylphosphine oxide (OPPh₃) as low molar mass flame retardants. The glass transition temperature, thermal decomposition, flammability (reaction to small flame), and burning behavior of the thermosets are investigated using differential scanning calorimetry, thermogravimetric analysis, pyrolysis combustion flow calorimetry, UL 94-burning chamber testing, and cone calorimeter measurements. Although P-contents are low at only 0.6 wt%, the study aims not at attaining V-0, but at presenting a proof of principle: Epoxy resinswith PDTT show promising fire performance, exhibiting a 25% reduction in total heat evolved (THE), a 30% reduction in peak heat release rate (PHRR) due to flame inhibition (21% reduction in effective heat of combustion (EHC)), and an increase in T_g at the same time. This study indicates that rigid aromatic hyperbranched polymeric structures offer a promising route toward multifunctional flame retardancy.     

Food fermentations: microorganisms with technological beneficial use

Microbial food cultures have directly or indirectly come under various regulatory frameworks in the course of the last decades. Several of those regulatory frameworks put emphasis on "the history of use", "traditional food", or "general recognition of safety". Authoritative lists of microorganisms with a documented use in food have therefore come into high demand. One such list was published in 2002 as a result of a joint project between the International Dairy Federation (IDF) and the European Food and Feed Cultures Association (EFFCA). The "2002 IDF inventory" has become a de facto reference for food cultures in practical use. However, as the focus mainly was on commercially available dairy cultures, there was an unmet need for a list with a wider scope. We present an updated inventory of microorganisms used in food fermentations covering a wide range of food matrices (dairy, meat, fish, vegetables, legumes, cereals, beverages, and vinegar). We have also reviewed and updated the taxonomy of the microorganisms used in food fermentations in order to bring the taxonomy in agreement with the current standing in nomenclature.     

Biocontrol of Biofilm Formation: Jamming of Sessile-Associated Rhizobial Communication by Rhodococcal Quorum-Quenching

Biofilms are complex structures formed by a community of microbes adhering to a surface and/or to each other through the secretion of an adhesive and protective matrix. The establishment of these structures requires a coordination of action between microorganisms through powerful communication systems such as quorum-sensing. Therefore, auxiliary bacteria capable of interfering with these means of communication could be used to prevent biofilm formation and development. The phytopathogen Rhizobium rhizogenes, which causes hairy root disease and forms large biofilms in hydroponic crops, and the biocontrol agent Rhodococcus erythropolis R138 were used for this study. Changes in biofilm biovolume and structure, as well as interactions between rhizobia and rhodococci, were monitored by confocal laser scanning microscopy with appropriate fluorescent biosensors. We obtained direct visual evidence of an exchange of signals between rhizobia and the jamming of this communication by Rhodococcus within the biofilm. Signaling molecules were characterized as long chain (C₁₄) N-acyl-homoserine lactones. The role of the Qsd quorum-quenching pathway in biofilm alteration was confirmed with an R. erythropolis mutant unable to produce the QsdA lactonase, and by expression of the qsdA gene in a heterologous host, Escherichia coli. Finally, Rhizobium biofilm formation was similarly inhibited by a purified extract of QsdA enzyme.     

Arbutus species (Ericaceae) as source of valuable bioactive products

Abstract

In addition to nutrients, plant foods contain compounds that may provide additional health benefits improving the quality of life. Species from Arbutus genus (Ericaceae) represent a promising source of healthy phytochemicals. Bioactive compounds including such as anthocyanins, iridoids, phenols, triterpenes, sterols, and fatty acids are reported from Arbutus species. Some Arbutus species revealed promising biological activities including antioxidant, anti-inflammatory, anti-proliferative, anti-diabetic, and antimicrobial activities, and deserve for that reason further consideration for new drug discovery. However, only few species are investigated scientifically for their chemical profile and biological activities. The aim of this article is to summarize the current knowledge of the components and biological properties of Arbutus species common in Mediterranean area, as well as the future prospects on their applications as potentially valuable products.     

Experimental and numerical investigations of an oxygen single-bubble shrinkage in a borosilicate glass-forming liquid doped with cerium oxide

The shrinkage of an oxygen single-bubble is investigated in a cerium-doped borosilicate glass melt at 1150°C. Nine glass samples are synthesized and investigated, utilizing three different amounts of Ce₂O₃ and three different redox ratios (Ce-(III)/Ce_total). Employing in-situ observation, the single-bubble behavior is recorded with a camera. For each glass melt, five experiments are performed with different initial bubble radii. The shrinkage rate () depends strongly on the cerium content as well as the redox ratio. Numerical calculations are also conducted to support the understanding of the bubble shrinkage mechanism in the given cases. The model adequately estimates the experimental data for several cases, and an explanation is proposed for the cases, in which it does not. Moreover, we demonstrate, physically and mathematically, the influence of the initial radius of the bubble on the mass transfer between the rising bubble and the melt. We confirm the utilization of the “modified Péclet number,” which is a dimensionless number that takes into consideration the influence of multivalent elements on mass transfer. Finally, we master the bubble shrinkage behavior by normalizing the experimental data employing a characteristic time for the mass transfer (τ).