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排序方式: 共有530条查询结果,搜索用时 15 毫秒
61.
High-performance genetic analysis on microfabricated capillary array electrophoresis plastic chips fabricated by injection molding 总被引:1,自引:0,他引:1
Dang F Tabata O Kurokawa M Ewis AA Zhang L Yamaoka Y Shinohara S Shinohara Y Ishikawa M Baba Y 《Analytical chemistry》2005,77(7):2140-2146
We have developed a novel technique for mass production of microfabricated capillary array electrophoresis (mu-CAE) plastic chips for high-speed, high-throughput genetic analysis. The mu-CAE chips, containing 10 individual separation channels of 50-microm width, 50-microm depth, and a 100-microm lane-to-lane spacing at the detection region and a sacrificial channel network, were fabricated on a poly(methyl methacrylate) substrate by injection molding and then bonded manually using a pressure-sensitive sealing tape within several seconds at room temperature. The conditions for injection molding and bonding were carefully characterized to yield mu-CAE chips with well-defined channel and injection structures. A CCD camera equipped with an image intensifier was used to monitor simultaneously the separation in a 10-channel array with laser-induced fluorescence detection. High-performance electrophoretic separations of phiX174 HaeIII DNA restriction fragments and PCR products related to the human beta-globin gene and SP-B gene (the surfactant protein B) have been demonstrated on mu-CAE plastic chips using a methylcellulose sieving matrix in individual channels. The current work demonstrated greatly simplified the fabrication process as well as a detection scheme for mu-CAE chips and will bring the low-cost mass production and application of mu-CAE plastic chips for genetic analysis. 相似文献
62.
63.
The paper reports an experiment which demonstrates the possibility of error-free transmission over 100 km without repeater, by using a single-mode fiber and a laser diode with distributed feedback emitting a single longitudinal mode at the wavelength 1.5 μm. 相似文献
64.
Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors 下载免费PDF全文
Kensuke Kondo Tatsuro Ishida Tomoyuki Yasuda Hideto Nakajima Kenta Mori Nobuaki Tanaka Takeshige Mori Tomoko Monguchi Masakazu Shinohara Yasuhiro Irino Ryuji Toh Yoshiyuki Rikitake Kazunobu Kiyomizu Yoshiaki Tomiyama Junichiro Yamamoto Ken‐ichi Hirata 《Molecular nutrition & food research》2015,59(4):729-740
65.
The simultaneous differential equations which connected the copolymerization kinetics of the component reaction in the multi-copolymerization system with the changing rate of the concentration of each monomer and radical, the concentration and the molecular weight of the polymer obtained are analysed in a number of ways. The distribution of the components and the degree of polymerization are also simulated. The styrene/butyl methacrylate/methacrylic acid terpolymerization system, using carbon tetrachloride as solvent and benzoyl peroxide as initiator, was chosen. The simulation model which satisfied the experimental values of the concentration of the multiple components in the system, the composition and molecular weight distribution and the number of chlorine atoms introduced into the copolymer ends is established. When the time interval changed, the effects on the distribution of the composition in copolymers and the distribution of the degree of polymerization were also calculated. 相似文献
66.
Warner JH Ito Y Zaka M Ge L Akachi T Okimoto H Porfyrakis K Watt AA Shinohara H Briggs GA 《Nano letters》2008,8(8):2328-2335
The rotation of fullerene chains in SWNT peapods is studied using low-voltage high resolution transmission electron microscopy. Anisotropic fullerene chain structures (i.e., C300) are formed in situ in carbon nanopeapods via electron beam induced coalescence of individual fullerenes (i.e., C60). A low electron accelerating voltage of 80 kV is used to prevent damage to the SWNT. The large asymmetric C300 fullerene structure exhibits translational motion inside the SWNT and unique corkscrew like rotation motion. Another asymmetric fullerene chain containing mixed fullerene species is prepared by fusing smaller C60 fullerenes to a larger Sc@C82 fullerene, and this also exhibits corkscrew rotational motion. Chains of Sc3C2@C80 in SWNT peapods adopt a zigzag packing structure, and the entire zigzag chain rotates inside the SWNT to induce structural modifications to the SWNT diameter and cross-sectional shape of the SWNT. The expansion and contraction of the diameter of the SWNT is measured as 17%, demonstrating nanoactuation behavior in carbon nanopeapods. 相似文献
67.
Suzuki T. Ikeda H. Yoshida H. Shinohara S. 《Industrial Electronics, IEEE Transactions on》1999,46(6):1159-1173
This paper describes MOSFET power inverter systems, each provided to drive a megasonic transducer with an output power of 600 W at a frequency of 1 MHz. Since the megasonic transducer is used as a resonant load with a series resistance of approximately 1 Ω, the impedance characteristic of the megasonic transducer used as an inverter load is analyzed and compared with measured data. A new method is developed to match the inverter output impedance to the load impedance of as low as 1 Ω at the resonant frequency using a high-performance output transformer which can feed RF power to the load at high efficiency. The output transformer having a primary-to-secondary winding ratio of 2 to 1 was used by the inverter to drive the megasonic transducer. Based on the analysis of the impedance characteristics of the load, two types of MOSFET dc-to-RF power inverters, a full-bridge version and a single-ended version, were designed and built. These power inverters were put into practical use in cleaners. The power conversion efficiency was greater than 80% for the full-bridge version at an output of approximately 600 W and 50% for the single-ended version at an output power of approximately 600 W. When the megasonic transducer was operated with an input power of 600 W. the operation was satisfactory 相似文献
68.
Keiji Uchiyama Hideyuki Hara Junji Chida Agriani Dini Pasiana Morikazu Imamura Tsuyoshi Mori Hanae Takatsuki Ryuichiro Atarashi Suehiro Sakaguchi 《International journal of molecular sciences》2021,22(21)
Prion diseases are a group of fatal neurodegenerative disorders caused by accumulation of proteinaceous infectious particles, or prions, which mainly consist of the abnormally folded, amyloidogenic prion protein, designated PrPSc. PrPSc is produced through conformational conversion of the cellular isoform of prion protein, PrPC, in the brain. To date, no effective therapies for prion diseases have been developed. In this study, we incidentally noticed that mouse neuroblastoma N2a cells persistently infected with 22L scrapie prions, termed N2aC24L1-3 cells, reduced PrPSc levels when cultured in advanced Dulbecco’s modified eagle medium (DMEM) but not in classic DMEM. PrPC levels remained unchanged in prion-uninfected parent N2aC24 cells cultured in advanced DMEM. These results suggest that advanced DMEM may contain an anti-prion compound(s). We then successfully identified ethanolamine in advanced DMEM has an anti-prion activity. Ethanolamine reduced PrPSc levels in N2aC24L1-3 cells, but not PrPC levels in N2aC24 cells. Also, oral administration of ethanolamine through drinking water delayed prion disease in mice intracerebrally inoculated with RML scrapie prions. These results suggest that ethanolamine could be a new anti-prion compound. 相似文献
69.
Katsuji Shinohara Takashi Nagano Hiroki Arima W. Zaidi W. Mustafa 《Electrical Engineering in Japan》2001,135(1):56-64
The vector control of induction motors is widely used. This method needs accurate motor parameter but the stator and rotor resistance vary due to motor temperature variation. If the value of resistance in the controller can be set up accurately at first, there must be a difference between the reference and real value of torque because of drift of the resistance. It is necessary to adapt the resistance value. The indirect field‐oriented control of an induction motor requires the value of rotor resistance only, but the direct field‐oriented control method with rotor flux observer requires the value of not only rotor resistance but also stator resistance in the controller. Consequently, it is necessary to adapt both stator resistance and rotor resistance. A parameter adaptation scheme has previously been proposed for the direct field‐oriented control method with rotor flux observer, but this method cannot be used when the motor is in regenerating operation. In this paper, a new stator and rotor resistance adaptation scheme is proposed, which can be applied when the motor is in regenerating operation. The usefulness of the proposed adaptation scheme is confirmed by simulation. © 2001 Scripta Technica, Electr Eng Jpn, 135(1): 56–64, 2001 相似文献
70.
Interaction control between endohedral metallofullerene and metal substrate by introducing alkanethiol self-assembled monolayer 总被引:1,自引:0,他引:1
Yasutake Y Shi Z Okazaki T Shinohara H Majima Y 《Journal of nanoscience and nanotechnology》2006,6(11):3460-3463
The interaction control between endohedral metallofullerenes and a metal substrate has been demonstrated by introducing hexanethiol, octanethiol, and decanethiol self-assembled monolayers (SAMs) as the interlayer. We observe the electric properties of terbium endohedral metallofullerenes (Tb@C82) on alkanethiol SAMs with different chain lengths by scanning tunneling microscopy (STM) and spectroscopy (STS). Based on the comparison of the high-resolution STM images of a Tb@C82 molecule on hexanethiol and octanethiol SAMs, the interaction between Tb@C82 and a hexanethiol SAM is found to be larger than that between Tb@C82 and an octanethiol SAM; this is because at 68 K, the rotational states of Tb@C82 terminate only on the hexanethiol SAM. Furthermore, we find that the tunneling current-voltage characteristics of Tb@C82 on the hexanethiol SAM show the rectifying effects that are also caused by the molecular energy level shifts of Tb@C82 molecules due to the large interaction. 相似文献