Matrilin-3-Primed Adipose-Derived Mesenchymal Stromal Cell Spheroids Prevent Mesenchymal Stromal-Cell-Derived Chondrocyte Hypertrophy

Adipose-derived mesenchymal stromal cells (Ad-MSCs) are a promising tool for articular cartilage repair and regeneration. However, the terminal hypertrophic differentiation of Ad-MSC-derived cartilage is a critical barrier during hyaline cartilage regeneration. In this study, we investigated the role of matrilin-3 in preventing Ad-MSC-derived chondrocyte hypertrophy in vitro and in an osteoarthritis (OA) destabilization of the medial meniscus (DMM) model. Methacrylated hyaluron (MAHA) (1%) was used to encapsulate and make scaffolds containing Ad-MSCs and matrilin-3. Subsequently, the encapsulated cells in the scaffolds were differentiated in chondrogenic medium (TGF-β, 1–14 days) and thyroid hormone hypertrophic medium (T3, 15–28 days). The presence of matrilin-3 with Ad-MSCs in the MAHA scaffold significantly increased the chondrogenic marker and decreased the hypertrophy marker mRNA and protein expression. Furthermore, matrilin-3 significantly modified the expression of TGF-β2, BMP-2, and BMP-4. Next, we prepared the OA model and transplanted Ad-MSCs primed with matrilin-3, either as a single-cell suspension or in spheroid form. Safranin-O staining and the OA score suggested that the regenerated cartilage morphology in the matrilin-3-primed Ad-MSC spheroids was similar to the positive control. Furthermore, matrilin-3-primed Ad-MSC spheroids prevented subchondral bone sclerosis in the mouse model. Here, we show that matrilin-3 plays a major role in modulating Ad-MSCs’ therapeutic effect on cartilage regeneration and hypertrophy suppression.     

回字形环山地建筑结构设计

中央美院青岛创业中心项目为角部重叠的回字形山地建筑,采用了框架-剪力墙结构体系.主体建筑结构总高度为54m,坐落在三个台地上,每两个相邻台地高差10m左右.掉层处采用"掉层脱开式且有拉梁"的方式进行结构布置.结合山地建筑结构的基本概念和受力特点,介绍了本项目的结构处理方式和设计难点,采用PKPM,MIDAS Building,SAUSAGE软件对结构进行了弹性反应谱法分析、弹性时程分析、动力弹塑性时程分析和抗震性能设计,同时针对各种不利因素采取了相应的加强措施,确保了结构安全.     

Preparation of CaO granules using the granulation method

ABSTRACT

Improving the hydration resistance of CaO particle in manufacturing and application of free CaO-containing materials has practical significance. In this study, CaO granules was made from Ca(OH)₂ particles, which were fabricated by the granulation method. The results showed that the hydration resistance of the CaO granules which was prepared under 1700?r?min^?1 was the best, the CaO granules was sintered well in calcination process, the shell of CaO granules was relatively dense, which improves the hydration resistance of CaO granules, and the rate of hydration weight increment was 0.58% after placed in the air for 20 days under a temperature of 10–14°C and a relative humidity of 57–81%.     

Microwave dielectric properties of SrLa[Ga1−x(Mg0.5Ti0.5)x]O4 and SrLa[Ga1−x(Zn0.5Ti0.5)x]O4 (x = 0.2-0.8) ceramics

SrLa[Ga_1−x(R_0.5Ti_0.5)_x]O₄ (R = Mg, Zn) ceramics were prepared by a standard solid state sintering method. The single-phase ceramics with K₂NiF₄-type layered perovskite structure and I4/mmm space group were obtained, indicating that SrLa(R_0.5Ti_0.5) and SrLaGaO₄ can form the unlimited solid solutions. With increasing x for R = Mg and Zn, ε_r increases monotonously, the Qf value first increases and then decreases, while τ_f increases from a negative to a positive value. The optimized microwave dielectric properties were obtained as following: ε_r = 23.3, Qf = 89 400 GHz, τ_f = −0.8 ppm/°C for SrLa[Ga_0.6(Mg_0.5Ti_0.5)_0.4]O₄ and ε_r = 23.3, Qf = 76 200 GHz, τ_f = 0.2 ppm/°C for SrLa[Ga_0.7(Zn_0.5Ti_0.5)_0.3]O₄, indicating that the present solid solution ceramics are the promising candidates as microwave resonator materials for the telecommunication applications.     

太赫兹超表面计算全息

全息术是一种三维成像技术,它已经被应用于多种实际场景。随着计算机科学与技术的迅猛发展,计算全息由于其方便和灵活的特性,已经成为一种广泛应用的全息成像方法。本文回顾了我们近期基于超表面的太赫兹计算全息研究进展。其中,作为全息板的超表面展示出了超越传统光学器件的独特性能。首先,利用超表面实现了对于全息板每个像素的相位振幅同时且独立的调控,进而实现了高质量全息成像。这种新的电磁波操控能力也带来了新的全息成像效果,如利用介质超表面实现了全息像沿传播方向上的连续变化。其次,对超表面在不同偏振态下的响应进行设计,分别实现了线偏振态与频率复用、圆偏振态复用、以及基于表面波的偏振复用超表面全息术。此外,本文提出了依赖于温度变化而主动可控的超表面全息术,为今后计算全息术的设计与实现提供了新的方案,也推动了超表面在实际应用方面的发展。     

2019年12月重点联系玻璃企业产、销、存、价分析

2019年12月汇总平板玻璃企业生产平板玻璃2558.01万重量箱,同比上涨11.63%,累计生产平板玻璃29927.38万重量箱,同比增长6.17%;本月销售平板玻璃2595.55万重量箱,同比上涨12.10%,累计销售平板玻璃30218.99万重量箱,同比上涨7.59%;本月平板玻璃销售率为101.47%,比上年同期下降3.19个百分点;月末平板玻璃库存1798.73万重量箱,同比下降21.09%.     

Mixed-Metal MOF-74 Templated Catalysts for Efficient Carbon Dioxide Capture and Methanation

The vast chemical and structural tunability of metal–organic frameworks (MOFs) are beginning to be harnessed as functional supports for catalytic nanoparticles spanning a range of applications. However, a lack of straightforward methods for producing nanoparticle-encapsulated MOFs as efficient heterogeneous catalysts limits their usage. Herein, a mixed-metal MOF, NiMg-MOF-74, is utilized as a template to disperse small Ni nanoclusters throughout the parent MOF. By exploiting the difference in Ni O and Mg O coordination bond strength, Ni²⁺ is selectively reduced to form highly dispersed Ni nanoclusters constrained by the parent MOF pore diameter, while Mg²⁺ remains coordinated in the framework. By varying the ratio of Ni to Mg in the parent MOF, accessible surface area and crystallinity can be tuned upon thermal treatment, influencing CO₂ adsorption capacity and hydrogenation selectivity. The resulting Ni nanoclusters prove to be an active catalyst for CO₂ methanation and are examined using extended X-ray absorption fine structure and X-ray photoelectron spectroscopy. By preserving a segment of the Mg²⁺-containing MOF framework, the composite system retains a portion of its CO₂ adsorption capacity while continuing to deliver catalytic activity. The approach is thus critical for designing materials that can bridge the gap between carbon capture and CO₂ utilization.