Novel NGR anchored pullulan micelles for controlled and targeted delivery of doxorubicin to HeLa cancerous cells

Doxorubicin (DOX) is used to treat different kinds of cancers, including cervix carcinoma. However, it has various side effects such as cardiotoxicity. Nano-sized controlled releasing carriers such as polymeric micelles are of interesting approaches to overcome these side effects of doxorubicin in cancer chemotherapy. Regarding the up-regulation of CD13/APN receptors on the cervix carcinoma cells, which can bind to peptide sequences specially NGR (asparagine–glycine–arginine) with high affinity, peptide sequence (NGR) targeted micelles would lead to effective treatment of this carcinoma. In this study, the NGR peptide sequence was synthesized using the solution-phase strategy from asparagine, glycine, and arginine residues. The pullulan–retinoic acid conjugate and pullulan–retinoic acid–NGR conjugate were prepared by the amide and ester bond formation between the hydroxyl groups of pullulan and carboxylic acid groups of retinoic acid and peptide sequence. Pullulan–retinoic acid–NGR micelles were prepared by the direct dissolution method. The optimized micelles, according to their particle size (124.5 nm), zeta potential (? 3.65 mV), entrapment efficiency (85%), and release of DOX (70%, within 72 h) were assessed for their cytotoxicity on HeLa cells using MTT assay. NGR-targeted pullulan/retinoic acid micelles had higher cytotoxicity than the free DOX in cell culture studies on the HeLa cell line, and this can be a promising result in the treatment of cervix carcinoma.     

Potency of Different Carbon Sources in Reduction of Microsilica to Synthesize SiC from Mechanically Activated Powder Mixtures

In the present study, the influences of three different types of carbon (carbon black, graphite, and petroleum coke) on SiC synthesis via mechanical activation and sintering were evaluated. In this regard, the phase components, morphology, and the formation mechanism were investigated. SiC nanoparticles were detected to be formed after 4 h of milling and sintering at 1450°C, regardless of the sources of carbon. The carbon types exert their effects on the morphology of the as‐synthesized particles, where carbon black leads to form rod‐like SiC particles and the other two carbon types result in semi‐spherical SiC particles. This is due to the dominant mechanism in the mentioned process. The rod‐like particles obtained from the carbon black‐containing powder were synthesized via the VSL mechanism, whereas the solid‐state reactions occurred to form the SiC particles in the graphite‐ or petroleum coke‐containing samples. In the VSL mechanism, any increase in the milling time leads to facilitate the SiC formation due to entrance of Fe debris, whereas in the other samples (graphite or petroleum coke) the procedure is reversed.     

Thermosensitive folic acid-targeted poly (ethylene-<Emphasis Type="Italic">co</Emphasis>-vinyl alcohol) hemisuccinate polymeric nanoparticles for delivery of epirubicin to breast cancer cells

A novel thermosensitive folic acid (FA)-targeted succinylated poly (ethylene-co-vinyl alcohol) (EVOH) (EVOHS-FA) nanocarrier was synthesized for the specific delivery of epirubicin (EPI) to MCF-7 breast cancer cell line. Three different ratios of synthesized EVOH-Suc were reacted with FA. The structure of the desired products (EVOHS₄₀-FA, EVOHS₆₀-FA and EVOHS₈₀-FA) was confirmed by ¹H NMR and FTIR techniques. Nanoparticles were obtained by nano-precipitation procedure using DMSO/H₂O as solvent/anti-solvent. The particle size, zeta potential, entrapment efficacy and in vitro release profile of the final formulations in different temperatures were measured. The optimized nanoparticles had the particle size of 214 ± 8.5 nm, zeta potential of ?29.6 mV, PDI of 0.198 ± 0.04, and a high encapsulation efficiency that released the drug efficiently within 450 h at the temperature of 40 °C compared to 37 °C. The morphology of nanoparticles was studied by scanning electron microscopy. The in vitro cytotoxicity was evaluated using the MTT assay on MCF-7 cell lines in response to temperatures of 37 and 40 °C. The MTT assay indicated that the targeted nanoparticles carrying EPI were significantly more cytotoxic than the non-targeted nanoparticles and the free drug at 40 °C.     

Effects of cooling channel blockage on fuel plate temperature in Tehran Research Reactor

In this study, the variation of the temperature distribution of the fuel plate in Tehran Research Reactor core was studied in case of coolant channels blockage. While the experimental method is not possible, both the analytical and simulation methods were used to obtain the more reliable data. The results show that one channel blockage will increase the fuel temperature to about 100%, but it does not lead to clad melt down still. With further calculation and simulation it is understood that if the coolant velocity drops to 90% of its nominal value, it may causes the clad melt-ing down. At least two channels with complete blockage even at the positions far from the core center can also melt down the clad.     

Application of the differential transformation method and variational iteration method to large deformation of cantilever beams under point load

Large deflection of a cantilever beam subjected to a tip-concentrated load is governed by a non-linear differential equation. Since it is hard to find exact or closed-form solutions for this non-linear problem, this paper investigates the aforementioned problem via the differential transformation method (DTM) and the variational iteration method (VIM), which are well-known approximate analytical solutions. The mathematical formulation is yielded to a non-linear two-point boundary value problem. In this study, we compare the DTM and VIM results, with those of Adomian decomposition method (ADM) and the established numerical solution obtained by the Richardson extrapolation in order to verify the accuracy of the proposed methods. As an important result, it is depicted from tabulated data that the DTM results are more accurate in comparison with those obtained by the VIM and ADM, which is one of the objectives of this article. Moreover, the effects of dimensionless end point load, ??, on the slope of any point along the arc length and the dimensionless vertical and horizontal displacements are illustrated and explained. The results reveal that these methods are very effective and convenient in predicting the solution of such problems, and it is predicted that the DTM and VIM can find a wide application in new engineering problems.     

Novel solution for acceleration motion of a vertically falling spherical particle by HPM–Padé approximant

In this paper the acceleration motion of a vertically falling spherical particle in incompressible Newtonian media is investigated. The velocity is evaluated by using homotopy perturbation method (HPM) and Padé approximant which is an analytical solution technique. The current results are then compared with those derived from HPM and the established fourth order Runge–Kutta method in order to verify the accuracy of the proposed method. It is found that this method can achieve more suitable results in comparison to HPM.     

A new compression based approach for reconfiguration overhead reduction in virtex based RTR systems

The advantage of RTR systems usually comes with some costs. The required time to map some areas of a program to an FPGA is considerable and affects the performance of RTR systems. Several methods have been developed to speed up the configuration process in these systems. Configuration compression can reduce the total number of write operations to load a configuration and it has been proven to be an efficient technique for dealing with the configuration overhead. In this paper, we have developed a new approach for reconfiguration overhead reduction in Virtex Based RTR Systems by using a compression technique based on Lempel-Ziv (LZ) algorithm. Since the order of the sequence of configuration frames affects the compression rate, we have proposed an algorithm based on Genetic Algorithm for finding the optimal configuration sequence of frames. The proposed algorithm will be applied to the input configuration file in a batch (offline) manner, and its time complexity is tolerable considering the overhead reduction obtained by having the optimal sequence of frames in run-time configuration decompression. Also, corresponding to our approach, a hardware model has been designed for configuration decompression.     

Upper Limb Robot Mediated Stroke Therapy—GENTLE/s Approach

Stroke is a leading cause of disability in particular affecting older people. Although the causes of stroke are well known and it is possible to reduce these risks, there is still a need to improve rehabilitation techniques. Early studies in the literature suggest that early intensive therapies can enhance a patient's recovery. According to physiotherapy literature, attention and motivation are key factors for motor relearning following stroke. Machine mediated therapy offers the potential to improve the outcome of stroke patients engaged on rehabilitation for upper limb motor impairment. Haptic interfaces are a particular group of robots that are attractive due to their ability to safely interact with humans. They can enhance traditional therapy tools, provide therapy on demand and can present accurate objective measurements of a patient's progression. Our recent studies suggest the use of tele-presence and VR-based systems can potentially motivate patients to exercise for longer periods of time. The creation of human-like trajectories is essential for retraining upper limb movements of people that have lost manipulation functions following stroke. By coupling models for human arm movement with haptic interfaces and VR technology it is possible to create a new class of robot mediated neuro rehabilitation tools. This paper provides an overview on different approaches to robot mediated therapy and describes a system based on haptics and virtual reality visualisation techniques, where particular emphasis is given to different control strategies for interaction derived from minimum jerk theory and the aid of virtual and mixed reality based exercises.     

Brittle fracture assessment of engineering components in the presence of notches: a review

Brittle fracture of notched components has been widely investigated in recent decades both experimentally and theoretically. This is because of designers' concern about catastrophic failure in notched engineering components made of brittle or quasi‐brittle materials. Up to now, extensive studies have been performed on brittle fracture analysis of engineering components weakened by notches of various features under mode I, mode II, mode III and mixed mode loading conditions. In the present paper, the attempt is made to review the research articles published in the open literature on brittle fracture assessment of notched components by means of notch fracture mechanics concepts. The main focus of this paper is on the stress‐based fracture criteria, which are the basis of authors' experience in recent years.