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151.
In Cuba, since 1959, the energy, environment and socio-economic development have been given high priority in national development plans.Fifty years later, the Cuban people have achieved a society with notable advances in literacy and education, health, culture, sports, social security and per capita of the gross domestic product (GDP), which has permitted the attainment of a sustainable development, according to the World Wildlife Fund (WWF) report in 2006.The energy sector has been evolving constantly in the last 50 years, achieving as the main result the distribution of electricity to 97% of the country, the growth of domestic oil production to achieve about 47% of the total consumption, the introduction of the distributed generation of electricity reaching 40% on the generation, rehabilitation of the electric appliances, systematization of the Cuban Electricity Conservation Program (PAEC), the Energy Conservation Program of the Ministry of Education (PAEME) and rapid introduction of renewable energy technologies, with good results in demand side management, energy efficiency and energy education.Actually, soil degradation, deforestation, pollution, loss of biological diversity and lack of water have been identified as the main environmental problems; several plans and projects have been applied, in order to reduce their impact, following the policy expressed in the National Environmental Strategy.However, challenges exist for future development in Cuba in coming years, from an economical point of view will be necessary the enhancement of the economic relations with the American and European countries, to solve internal problems such as insufficient productivity, correspondence between the level of activities with the financial, material and human resources, to promote growth in the levels of exports and to achieve the substitution of imported basic food; the energy sector need to achieve growth in the levels of prospection and exploitation of domestic oil, to diversify fossil energy and energetic technologies suppliers, energy efficiency and the use of renewable energy; the protection of the environment will demand to develop research about adaptation and mitigation of climate change, conservation and rational use of natural resources, in particular, the lands, water and forests.  相似文献   
152.
    
NMR methods for the characterization of local protein motions have attained a high level of sophistication. Measurement of the synchronization between those motions, however, poses a serious challenge. Such correlated motions are one of the underlying mechanisms for the propagation of local changes to remote sites and as such for information transfer. Here, we demonstrate the experimental detection of the synchronization of motion over an intermediate range. To that purpose, we designed pulse sequences for the measurement of cross-correlated relaxation between the backbone HN−N and side-chain Hβ−Cβ dipoles in Ile, Thr, and Val in the protein GB3. These bonds are related through two and three intervening dihedral angles. We show that the correlated motions inherent in a structural ensemble obtained from a large and diverse array of NMR probes are in excellent agreement with our measurements.  相似文献   
153.
    
The bacterial second messenger cyclic diguanosine monophosphate (c-di-GMP) is a key regulator of cellular motility, the cell cycle, and biofilm formation with its resultant antibiotic tolerance, which can make chronic infections difficult to treat. Therefore, diguanylate cyclases, which regulate the spatiotemporal production of c-di-GMP, might be attractive drug targets for control of biofilm formation that is part of chronic infections. We present a FRET-based biochemical high-throughput screening approach coupled with detailed structure–activity studies to identify synthetic small-molecule modulators of the diguanylate cyclase DgcA from Caulobacter crescentus. We identified a set of seven small molecules that regulate DgcA enzymatic activity in the low-micromolar range. Subsequent structure–activity studies on selected scaffolds revealed a remarkable diversity of modulatory behavior, including slight chemical substitutions that reverse the effects from allosteric enzyme inhibition to activation. The compounds identified represent new chemotypes and are potentially developable into chemical genetic tools for the dissection of c-di-GMP signaling networks and alteration of c-di-GMP-associated phenotypes. In sum, our studies underline the importance of detailed mechanism-of-action studies for inhibitors of c-di-GMP signaling and demonstrate the complex interplay between synthetic small molecules and the regulatory mechanisms that control the activity of diguanylate cyclases.  相似文献   
154.
    
Fluorescence‐based organic light‐emitting diodes (OLEDs) using thermally activated delayed fluorescence (TADF) have increasingly attracted attention in research and industry. One method to implement TADF is based on an emitter layer composed of an exciplex host and a fluorescent dopant. Even though the experimental realization of this concept has demonstrated promising external quantum efficiencies, the full potential of this approach has not yet been assessed. To this end, a comprehensive electro‐optical device model accounting for the full exciton dynamics including triplet harvesting and exciton quenching is presented. The model parameters are fitted to multiple output characteristics of an OLED comprising a TADF exciplex host with a fluorescent emitter, showing an external quantum efficiency of >10%. With the model at hand, an emission zone analysis and a parameter study are performed, and possible routes for further efficiency enhancement are presented.  相似文献   
155.
    
Protein–nucleic acid interactions play important roles not only in energy-providing reactions, such as ATP hydrolysis, but also in reading, extending, packaging, or repairing genomes. Although they can often be analyzed in detail with X-ray crystallography, complementary methods are needed to visualize them in complexes, which are not crystalline. Here, we show how solid-state NMR spectroscopy can detect and classify protein–nucleic interactions through site-specific 1H- and 31P-detected spectroscopic methods. The sensitivity of 1H chemical-shift values on noncovalent interactions involved in these molecular recognition processes is exploited allowing us to probe directly the chemical bonding state, an information, which is not directly accessible from an X-ray structure. We show that these methods can characterize interactions in easy-to-prepare sediments of the 708 kDa dodecameric DnaB helicase in complex with ADP:AlF4:DNA, and this despite the very challenging size of the complex.  相似文献   
156.
    
Intrinsic photogeneration of charge carriers in organic semiconductors is generally attributed to high energy ionization or exciton dissociation by a strong electric field. Here, high bulk photoconductivity is reported in pristine pentamethine cyanine films with photocurrent onset at the band‐edge of the organic semiconductor. Single‐layer cyanine diodes with selective hole and electron contacts show linear dependence of photocurrent with reverse voltage and light intensity. Numerical drift‐diffusion simulations reveal that the linear resistor behavior stems from low and unbalanced carrier mobilities giving rise to negative space charge. Slow bimolecular recombination kinetics of photoinduced charges obtained by time delayed charge extraction measurements show strongly reduced Langevin recombination with long carrier lifetime of the order of a millisecond. Such reduced charge carrier recombination puts forward a materials concept to be exploited in photodiodes and more generally in optoelectronic devices.  相似文献   
157.
    
Bisphenol F (BPF) was found in mustard up to a concentration of around 8 mg kg?1. Contamination of the raw products or caused by the packaging could be ruled out. Also, the fact that only the 4,4?-isomer of BPF was detected spoke against contamination from epoxy resin or other sources where technical BPF is used. Only mild mustard made of the seeds of Sinapis alba contained BPF. In all probability BPF is a reaction product from the breakdown of the glucosinolate glucosinalbin with 4-hydroxybenzyl alcohol as an important intermediate. Hot mustard made only from brown mustard seeds (Brassica juncea) or black mustard seeds (Brassica nigra) contained no BPF. BPF is structurally very similar to bisphenol A and has a similar weak estrogenic activity. The consumption of a portion of 20 g of mustard can lead to an intake of 100–200 µg of BPF. According to a preliminary risk assessment, the risk of BPF in mustard for the health of consumers is considered to be low, but available toxicological data are insufficient for a conclusive evaluation. It is a new and surprising finding that BPF is a natural food ingredient and that this is the main uptake route. This insight sheds new light on the risk linked to the family of bisphenols.  相似文献   
158.
159.
Context-aware platform for mobile data management   总被引:1,自引:0,他引:1  
Interaction design is a major issue for mobile information systems in terms of not only the choice of input/output channels and presentation of information, but also the application of context-awareness. To support experimentation with these factors, we have developed platforms to support the rapid prototyping of multi-channel, multi-modal, context-aware applications. The Java-based platform presented here is based on an integration of a cross-media link server and an object-oriented framework for advanced content publishing, along with a Client Controller and Context Engine. We also describe how this platform was used to develop a mobile tourist information system for an international arts festival where interaction was based on a combination of interactive paper and speech output. Moira C. Norrie is a Professor at ETH Zurich where she is head of the Institute for Information Systems and leads the Global Information Systems research group. Her research interests include object-oriented models and systems for data management, web engineering, mobile and personal information systems and interactive paper as a medium for integrating printed and digital information. Beat Signer is a Post-Doctoral researcher in the Global Information Systems research group at ETH Zurich. He received a Ph.D. from ETH Zurich in 2005 for his work investigating fundamental concepts for interactive paper and cross-media information management. His research interests include interactive paper, cross-media information management, object-oriented technologies and software engineering. Michael Grossniklaus is a research assistant in the Global Information Systems research group at ETH Zurich. He received a Diploma (M.Sc.) in Computer Science from ETH Zurich in 2001 and is currently completing his Ph.D. His main research interest is empowering information systems for context-aware data management and delivery in the domain of web engineering and mobile computing. Rudi Belotti was a research assistant in the Global Information Systems research group at ETH Zurich from 2004–2006. He received a Diploma (M.Sc.) in Computer Science from ETH Zurich in 2004. In his research, he developed a general model and engine for the management of context information in mobile information systems. He is currently working for an e-business services company in Ticino, Switzerland. Corsin Decurtins is a research assistant in the Global Information Systems research group at ETH Zurich. He received a Diploma (M.Sc.) in Computer Science from ETH Zurich in 2002. His research focusses on model-based approaches and infrastructure for ubiquitous and mobile information environments. In addition to his Ph.D. Corsin also works part-time as a senior software engineer at the software company Netcetera. Nadir Weibel is a research assistant in the Global Information Systems research group at ETH Zurich. He received a Diploma (M.Sc.) in Computer Science from ETH Zurich in 2003 and is currently working on his Ph.D. His research is in the area of interactive paper, particularly on the authoring and publishing infrastructure for interactive documents as well as issues of human computer interaction and mobile environments.  相似文献   
160.
Distinct layers with accumulated iron and manganese oxyhydroxides are found in the recent sediments of Lake Baikal (Siberia). In the South and Central Basins, these concretions accumulate close to the sediment-water interface. In northern Lake Baikal and the area of Academician Ridge, however, massive Fe/Mn crusts are formed within several thousand years at redox fronts 10 to 15 cm below the sediment surface. In some places, precipitated iron and manganese oxyhydroxides are spatially separated. The patterns are a result of secondary iron and manganese oxide precipitation. This natural long-term experiment allows the analysis of competitive adsorption and coprecipitation of trace elements with iron and manganese oxides in sediments. Background concentrations in the sediment of oxoanions (P, As, Sb, Mo); of trace metals (Cr, V, Cu, Zn, Cd, Pb); and of Mg, Ca, Sr, La, Ce, Pr, Nd, and Sm were analyzed by inductively coupled plasma mass spectrometry. Despite the differences in catchment geology of the many tributaries, they are remarkably uniform in sediment cores from different basins of Lake Baikal. Enrichment factors of P and As within Fe crusts revealed concentrations up to 14 and 58 times higher than the background, respectively. No enrichment of P and As was found in the Mn layers. By contrast, Mo accumulated exclusively in the Mn layer with up to 35-fold enrichment. Sb was only slightly enriched in both the Fe and the Mn layers. Among the trace metals studied, only Cd was found at elevated concentrations with a preference for the Mn layer. Ca and Sr were correlated with both Fe and Mn accumulations. The study quantifies the well-known specific adsorption and coprecipitation of P and As at authigenic iron oxides and of Mo on manganese oxides. In addition, the enrichment of Cd at manganese oxides in contrast to the conservative behavior of Zn and Pb reveals highly selective accumulation processes.  相似文献   
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